Gut microbiota diversity after autologous fecal microbiota transfer (a-FMT) in acute myeloid leukemia patients
Nature Communications - The combination of chemotherapy and broad-spectrum antibiotics induces gut microbiota (GM) dysbiosis in acute myeloid leukaemia (AML) leading to additional complications....
strong negative correlation between fecal neopterin and alpha diversity, butyrate producing bacteria and Bifidobacterium.
pro-inflammatory bacteria Enterococcus correlates positively with fecal neopterin and ferritin and negatively with the butyrate producing bacteria Roseburia
Akkermansia that has been inversely associated with obesity, diabetes, cardiometabolic diseases and low-grade inflammation21, but also in response to cancer immunotherapy
negatively correlate with inflammatory parameters (fecal neopterin, CD14+ monocytes and neutrophils)
development of allogenic FMT from multiple donors
AFMT may not be appropriate in patients with previous antibiotic therapies or other treatments altering the composition of the gut microbiota
Therefore, the use of allogenic FMT may be more effective
24 h after antibiotics discontinuation
AFMT was planned at the end
laxative was administered before the first procedure
2 inocula of 150 mL one day apart
each containing 30 g of feces
lying position on their left side
in this position for 20 min
afterward turn on their belly, on their back and on their right side, maintaining each position for 15 min
stored frozen at −80 °C until transplantation
Feces were processed within 72 h with a patented cryoprotective diluent
