Background: Skin aging can be caused by long-term ultraviolet (UV) exposure that usually called as photoaging. Administered antioxidant substances can delay the photoaging process. Purple mangosteen is known to have abundant antioxidant properties such as xanthones and anthocyanins. This study aims to determine the effect of purple mangosteen peel extract on the collagen fiber in male Wistar rats after UV-B exposure.Methods: An experimental study was conducted among 21 male Wistar rats at Histology Laboratorium, Udayana University from a period of March-October 2017. The samples were divided into 3 groups as follows: control, placebo, and mangosteen extract with 7 rats in each group. Collagen fiber was assessed by using a light microscope with 400 times magnification. Statistical analysis was carried out using SPSS ver. 21 software using one way ANOVA.Results: The study found that percentage of collagen area within control group was 61.94%, placebo group 72.90%, and mangosteen peel extract 73.63%. The mean between-group analyzed using one way ANOVA found that there was a statistically significant difference in mangosteen peel extract than control group (p=0.0000), but not statistically significant if compared with placebo group (p=0.640) on the collagen fiber in male Wistar rats.Conclusion: there was a statistically significant difference in mangosteen peel extract than control group on the thickness of collagen fiber in male Wistar rats after UV-B exposure.

Excessive Ultraviolet (UV) irradiation induces skin damage. In the present study, the potential protective activity of anthocyanins (cyanidin-3-O-sophoroside and cyanidin-3-O-sambubioside) from black peanut against skin damage induced by UV-B was evaluated in vitro and in vivo. Treatment with anthocyanins si

A multitude of extrinsic environmental factors (referred to in their entirety as the ‘skin exposome’) impact structure and function of skin and its corresponding cellular components. The complex (i.e. additive, antagonistic, or synergistic) ...

Background: UV-B irradiation is the major causative factor in photoaging. Photoaging increases MMP-1 significantly and cause damage to collagen by forming ROS. The monounsaturated free fatty acids and antioxidant compounds in Tamanu oil have been studied to inhibit the emergence of MMP-1 and maintain collagen amount. The study aimed to investigate topical Tamanu oil in inhibiting MMP-1 expressions and collagen decrease in male Wistar rats’ skin exposed to UV-B. Methods: A randomized post-test-only control group design was performed on 36 male Wistar rats, aged 2-3 months old with 180-220 grams body weight. They were assigned to two groups: the placebo group and the Tamanu oil group. Both groups were given the same amount of UV-B exposure for four weeks totalling in 840 mJ/cm2 of dosage radiation. Their dermis was examined for MMP-1 using Immunohistochemistry and collagen amount using Picro Sirius Red staining. Results: A comparative analysis was done to show the difference between groups. The mean expression of MMP-1 in the control group was 34,03±2.97 cell/HPF and the Tamanu oil group was lower at 22.73±2.68 cell/HPF. The mean amount of collagen dermis in the control group was 38.90±11.64 % and the Tamanu oil group was higher at 65.66±8.13 %. A significant difference was shown in the two groups (p Conclusion: Topical Tamanu oil has proven to hinder the increase of MMP-1 expressions and collagen amount in male Wistar rats’ skin exposed to UV-B.

Oil from the nuts of Calophyllum inophyllum, locally called "Tamanu oil" in French Polynesia, was traditionally used for wound healing and to cure various skin problems and ailments. The skin-active effect of "Tamanu oil emulsion" was investigated on human skin cells (keratinocytes and dermal fibroblasts) and showed cell proliferation, glycosaminoglycan and collagen production, and wound healing activity. Transcriptomic analysis of the treated cells revealed gene expression modulation including genes involved in the metabolic process implied in O-glycan biosynthesis, cell adhesion, and cell proliferation. The presence of neoflavonoids as bioactive constituents in Tamanu oil emulsion may contribute to these biological activities. Altogether, consistent data related to targeted histological and cellular functions brought new highlights on the mechanisms involved in these biological processes induced by Tamanu oil effects in skin cells.