First Light Diagnostics Receives FDA Clearance for its Rapid Clostridiodes difficile Test on the MultiPath® Analyzer - KPVI News 6
C Diff Diagnosis
Featured CE: Payer Expert James Kenney, RPh on C. Diff Costs
Calprotectin Stool Test: Uses, Procedure, Results & More - Healthline
The calprotectin stool test is a noninvasive test that can help determine if you have an inflammatory or noninflammatory bowel condition. Learn more about what this test measures and when it's used.
Using a nurse-initiated bedside tool to decrease inappropriate testing for Clostridioides difficile in hospital settings
Overdiagnosis of Clostridioides difficile (C. difficile) is associated with increased hospital length of stay, antibiotic overuse, unnecessary infection prevention efforts and excess costs. This study evaluated a paper-based bedside C. difficile screening tool on the number of …
Reductions in positive Clostridioides difficile events reportable to National Healthcare Safety Network (NHSN) with adoption of reflex enzyme immunoassay (EIA) testing in 13 Atlanta hospitals
Performance of commercial PCR assays to detect toxigenic Clostridioides difficile in the feces of puppies
Changes In Electronic Health Records (EHR) To Guide Clinicians In C. diff. Diagnostic Stewardship – To Pause Testing When Not Clinically Indicated | C Diff Foundation
An intervention that required administrative approval of all Clostridioides difficile testing after hospital day 3 out-performed electronic health record-based support in reducing C. difficile test…
Test restrictions more effective than EHR-based support at reducing C. difficile testing - Healio
An intervention that required administrative approval of all Clostridioides difficile testing after hospital day 3 out-performed electronic health record-based support in reducing C. difficile testing, according to a study.“We performed this work as part of a larger quality improvement initiative with the goal of improving the accuracy of diagnosis of C. difficile infection in order to
Evaluation of loop-mediated isothermal amplification assay for detection of Clostridioides difficile infection: A prospective diagnostic performance study
A novel Loop-mediated isothermal amplification (LAMP) assay, HiberGene's CD was evaluated with 82 unformed stools from patients suspected of C. difficile infection (CDI). Compared to glutamate dehydrogenase (GDH) toxins A/B test (C.diff Quik Chek®), HiberGene's LAMP showed 100% of sensitivity and 95 …
Detection of Clostridioides difficile in hospital environment by using C diff Banana Broth
116 environmental samples from a 504 bed clinical hospital obtained in 2017/19 were inoculated into C diff Banana Broth™. Six C. difficile and 12 C. pefringens strains were isolated. Antibiotic-resistant Clostridium spp. dominated in hospital environment. To determine Clostridium spp. in hospital en …
Optimization of an Assay To Determine Colonization Resistance to Clostridioides difficile in Fecal Samples from Healthy Subjects and Those Treated with Antibiotics | Antimicrobial Agents and Chemotherapy
A healthy, intact gut microbiota is often resistant to colonization by gastrointestinal pathogens. During periods of dysbiosis, however, organisms such as Clostridioides difficile can thrive. We describe an optimized in vitro colonization resistance assay for C. difficile in stool (CRACS) and demonstrate the utility of this assay by assessing changes in colonization resistance following antibiotic exposure. Fecal samples were obtained from healthy volunteers (n = 6) and from healthy subjects receiving 5 days of moxifloxacin (n = 11) or no antibiotics (n = 10). Samples were separated and either not manipulated (raw) or sterilized (autoclaved or filtered) prior to inoculation with C. difficile ribotype 027 spores and anaerobic incubation for 72 h. Different methods of storing fecal samples were also investigated in order to optimize the CRACS. In healthy, raw fecal samples, incubation with spores did not lead to increased C. difficile total viable counts (TVCs) or cytotoxin detection. In contrast, increased C. difficile TVCs and cytotoxin detection occurred in sterilized healthy fecal samples or those from antibiotic-treated individuals. The CRACS was functional with fecal samples stored at either 4°C or −80°C but not with those stored with glycerol (12% or 30% [vol/vol]). Our data show that the CRACS successfully models in vitro the loss of colonization resistance and subsequent C. difficile proliferation and toxin production. The CRACS could be used as a proxy for C. difficile infection in clinical studies or to determine if an individual is at risk of developing C. difficile infection or other potential infections occurring due to a loss of colonization resistance.
Transition From PCR-Ribotyping to Whole Genome Sequencing Based Typing of Clostridioides difficile
Clostridioides difficile causes nosocomial outbreaks which can lead to severe and even life-threatening colitis. Rapid molecular diagnostic tests allow the identification of toxin-producing, potentially hypervirulent strains, which is critical for patient management and infection control. PCR …
A review of the role of Gastrointestinal Multiplex PCR in the management of Diarrheal illness
Acute and chronic diarrheal illness secondary to gastrointestinal infection is a significant cause of morbidity and mortality around the world. A cornerstone of management includes prompt diagnosis and appropriate treatment of culprit pathogens. Timely diagnosis can improve patient care, assist in i …
Is Clostridioides difficile toxins detection necessary when the glutamate dehydrogenase enzyme is detected?
Editorial Article: Syndromic testing: Achieving better clinical outcomes for gastrointestinal pediatric patients - SelectScience
Dr. Samuel Dominguez shares the importance of robust testing methods and diagnostic stewardship
A cost of illness comparison for toxigenic Clostridioides difficile diagnosis algorithms in developing countries - PubMed
The two-step algorithm with simultaneous GDH and toxin A/B rapid immunoassay arbitrated by NAAT seems to be the best strategy for CDI diagnosis in developing countries.
In pursuit of the holy grail: Improving C. difficile testing appropriateness with iterative electronic health record clinical decision support and targeted test restriction
Test restriction was more effective than EHR-based clinical decision support to reduce C. difficile testing in our 3-hospital system.
How Did COVID-19 Affect Clostridioides Difficile Testing and Incidence Rates?
During the COVID-19 surge last year, the federal government stopped the required reporting on healthcare-associated infections (HAI) thus leaving an absence of data and important information on HAI. One study examined the effect the initial pandemic surge had on Clostridioides Difficile infection (CDI) in one hospital.
Molecular Clostridium Difficile Diagnostic Equipment Market Pegged for Robust Expansion by 2027 Covid-19 Analysis – The Courier - The Courier
C. Difficile: A Differential Diagnosis - Pharmacy Times
Important things to understand about diagnosing C. difficile and recommendations for properly differentiating the condition from other diseases when working-up a patient.
Testing for C. Difficile Infections - Pharmacy Times
Types of testing algorithms used in clinical practice to help clarify a diagnosis of C difficile infection.
Screening for C. Difficile - Pharmacy Times
A panel of clinicians recognize the challenges associated with screening patients for the C. difficile infection and highlight factors that should prompt someone for an evaluation.
Missed Diagnoses Common for C. Diff Infections - MD Magazine
Missed diagnosis resulted in delays in initiation of therapy and hospital re-admissions.
White Blood Cell Count Elevation Not Enough for C diff Testing - MD Magazine
“A high or rising white blood cell count in isolation is not a sufficient indication for C diff infection testing,” the study authors said.
Change in Guidelines Double Ability to Predict C. Diff Mortality - Pharmacy Times
The change in guidelines address how to assess damage to the kidneys caused by fluid loss associated with severe C. diff infection.
KBSI researchers develop super bacteria fast test kit - Korea Biomedical Review
The Korea Basic Science Institute (KBSI) said its researchers have developed a fast test kit that detects antibiotic-resistant super bacteria, Clostridioides Difficile (C.Difficile). The researcher...
Trends in Clostridioides difficile diagnosis before and after a change in testing algorithm
Clostridioides difficile (Clostridium difficile) (CD) infection remains a challenging diagnosis in hospitalized patients given the myriad of testing procedures and array of alternative causes for diarrhea. We identified 100 consecutive inpatients with positive CD testing in a single tertiary center …
Trends in Clostridioides difficile diagnosis before and after a change in testing algorithm
Clostridioides difficile (Clostridium difficile) (CD) infection remains a challenging diagnosis in hospitalized patients given the myriad of testing procedures and array of alternative causes for diarrhea. We identified 100 consecutive inpatients with positive CD testing in a single tertiary center …
Diagnostic syndromic multiplex approaches for gastrointestinal infections
Introduction: Gastrointestinal diseases due to infectious pathogens currently represent an important global health concern, especially in children and developing countries. Early and accurate detection of gastrointestinal pathogens is important to initiate the appropriate type of therapy. Mul …
The Role of Diagnostic Stewardship in Clostridioides difficile Testing: Challenges and Opportunities
CDI is a clinical diagnosis supported by laboratory findings. Together, clinical evaluation combined with diagnostic stewardship can optimize the accurate diagnosis of CDI.