One-pot synthesized three-way junction based multiple strand displacement amplification for sensitive assay of H5N1 DNA - PubMed
The rapid and sensitive detection of H5N1, a highly pathogenic avian influenza virus, is crucial for controlling its spread and minimizing its impact on public health. In this study, we developed a novel biosensor based on strand displacement amplification (SDA) coupled with CRISPR/Cas12a for highly …
Recent outbreaks of highly pathogenic avian influenza (also known as bird flu) have created a need for rapid and sensitive detection methods to mitigate its spread. Now, researchers have developed a prototype sensor that detects a type of influenza virus that causes bird flu (H5N1) in air samples. The low-cost handheld sensor detects the virus at levels below an infectious dose and could lead to rapid aerosol testing for airborne avian influenza.
WashU engineers make microwave-size bird flu detector amid outbreak
The researchers say the device could keep farmers from having to cull their flocks when they detect the contagious virus, which has affected more than 5 million birds in Missouri since 2022.
Applying Privacy Enhancing Tech to Help Identify Bird Flu
The adoption of privacy enhancing technologies, including fully homomorphic encryption, can help secure data as it is collected, integrated and shared for detecting
New biosensor can detect airborne bird flu in under five minutes
Researchers in the McKelvey School of Engineering at Washington University in St. Louis have developed a sensor that detects airborne H5N1 avian flu and can be used on poultry and dairy farms.
Capacitive Biosensor for Rapid Detection of Avian (H5N1) Influenza and E. coli in Aerosols - PubMed
Airborne transmission via aerosols is a dominant route for the transmission of respiratory pathogens, including avian H5N1 influenza A virus and iE. coli/i bacteria. Rapid and direct detection of respiratory pathogen aerosols has been a long-standing technical challenge. Herein, we develop a nov …
Air sampling accurately captures circulating zoonotic viral diversity emerging from poultry live-animal markets - PubMed
Environmental surveillance has emerged as a pivotal strategy for early detection of pathogens that pose threats to humans (1) but has not been utilized for zoonotic agents. In Asia, live-bird markets (LBMs) are key human-animal interfaces for zoonotic virus transmission (2). Traditional sampling str …
Air sampling detects 70–90% of poultry viruses and reveals 50 more hidden threats
Researchers found that environmental sampling in live-bird markets detects 70–90% of poultry viruses, often outperforming traditional swab methods, and identified 50 additional viruses, some from non-avian sources.
Capacitive Biosensor for Rapid Detection of Avian (H5N1) Influenza and E. coli in Aerosols
Airborne transmission via aerosols is a dominant route for the transmission of respiratory pathogens, including avian H5N1 influenza A virus and E. coli bacteria. Rapid and direct detection of respiratory pathogen aerosols has been a long-standing technical challenge. Herein, we develop a novel label-free capacitive biosensor using an interlocked Prussian blue (PB)/graphene oxide (GO) network on a screen-printed carbon electrode (SPCE) for direct detection of avian H5N1 and E. coli. A single-step electro-co-deposition process grows GO branches on the SPCE surface, while the PB nanocrystals simultaneously decorate around the GO branches, resulting in an ultrasensitive capacitive response at nanofarad levels. We tested the biosensor for H5N1 concentrations from 2.0 viral RNA copies/mL to 1.6 × 105 viral RNA copies/mL, with a limit of detection (LoD) of 56 viral RNA copies/mL. We tested it on E. coli for concentrations ranging from 2.0 bacterial cells/mL to 1.8 × 104 bacterial cells/mL, with a LoD of 5 bacterial cells/mL. The detection times for both pathogens were under 5 min. When integrated with a custom-built wet cyclone bioaerosol sampler, our biosensor could detect and quasi-quantitatively estimate H5N1 and E. coli concentrations in air with spatial resolutions of 93 viral RNA copies/m3 and 8 bacterial cells/m3, respectively. The quasi-quantification method, based on dilution and binary detection (positive/negative), achieved an overall accuracy of 90% for pathogen-laden aerosol samples. This biosensor is adaptable for multiplexed detection of other respiratory pathogens, making it a versatile tool for real-time airborne pathogen monitoring and risk assessment.
Pretorian Labs to Test Human Patients for H5N1 (Bird Flu)
/PRNewswire/ -- Pretorian Labs, LLC. is one of the first laboratories in the US to test human patients for H5N1 virus (Bird Flu). This test is now available to...
Evaluation of an N1 NA antibody-specific enzyme-linked lectin assay for detection of H5N1 highly pathogenic avian influenza virus infection in vaccinated birds - PubMed
Unprecedented H5N1 highly pathogenic avian influenza (HPAI) outbreaks are occurring around the world and there is growing interest in the use of vaccines in affected regions. Vaccination when properly applied can contribute to HPAI control by significantly reducing virus shedding and breaking the tr …
CDC Urges Hospitals to Speed Influenza A Subtyping for Bird Flu
This Medical News article discusses new guidance for clinicians and laboratories to prevent delays in detecting human cases of avian influenza A(H5N1) infection.
