2016

Studies have been focused on natural products with antibacterial and anti-inflammatory activities, such as fucoidan. Many in vivo studies have evaluat…

Fucoidan, a sulfated polysaccharide extracted from brown seaweed, has been used in traditional Chinese herbal medicine to treat thyroid tumors for many years. Although a number of its cellular effects have been investigated, the role of fucoidan in molecular signaling, particularly in Ca2+ signaling …

Fucoidan, a sulfated polysaccharide extracted from brown algae, exhibits anti-cancer activity. However, the effects and mechanism of fucoidan-induced apoptosis via endoplasmic reticulum (ER) stress is unclear. In this study, we demonstrated that fucoidan prevents tumorigenesis and reduces tumor size in LLC1-xenograft male C57BL/6 mice. Fucoidan induces an ER stress response by activating the PERK-ATF4-CHOP pathway, resulting in apoptotic cell death in vitro and in vivo. Furthermore, ATF4 knockdown abolishes fucoidan-induced CHOP expression and rescues cell viability. Specifically, fucoidan increases intracellular reactive oxygen species (ROS), which increase ATF4 and CHOP in lung cancer cells. Using the ROS scavenger N-acetyl-l-cysteine (NAC), we found that ROS generation is involved in fucoidan-induced ER stress-mediated apoptosis. Moreover, via Toll-like receptor 4 (TLR4) knockdown, we demonstrated that fucoidan-induced ROS and CHOP expression were attenuated. Our study is the first to identify a novel mechanism for the antitumor activity of fucoidan. We showed that fucoidan inhibits tumor viability by activating the TLR4/ROS/ER stress axis and the downstream PERK-ATF4-CHOP pathway, leading to apoptosis and suppression of lung cancer cell progression. Together, these results indicate that fucoidan is a potential preventive and therapeutic agent for lung cancer that acts via activation of ROS-dependent ER stress pathways.

Alzheimer's disease (AD) is the most common age-related neurodegenerative disorder. As the aging population is increasing, AD is becoming one of the leading causes of disability and death among the elderly. However, currently there is no cure for this disease. Fucoidan is a complex sulfated polysaccharide ma

Studies have been focused on natural products with antibacterial and anti-inflammatory activities, such as fucoidan. Many in vivo studies have evaluat…

Multiple myeloma (MM) remains an incurable hematological neoplasms. Our previous studies showed that Fucoidan possessed anti-myeloma effect by inducing apoptosis and inhibiting invasion of myeloma cells. In this study, we evaluated the effect of Fucoidan on angiogenesis induced by human myeloma cells and elucidated its possible mechanisms. Multiple myeloma cells were treated with Fucoidan at different concentrations, then the conditioned medium (CM) was collected. The levels of VEGF in the CM were tested by ELISA. The results showed that Fucoidan significantly decreased VEGF secretion by RPMI-8226 and U266 cells. The tube formation assay and migration test on human umbilical vein endothelial cells (HUVECs) were used to examine the effect of Fucoidan on angiogenesis induced by human myeloma cells. The results showed that Fucoidan decreased HUVECs formed tube structures and inhibited HUVECs migration, and suppressed the angiogenic ability of multiple myeloma RPMI-8226 and U266 cells in a dose-dependent manner. The study also showed that Fucoidan downregulated the expression of several kinds of proteins, which may be correlated with the reduction of angiogenesis induced by myeloma cells. Moreover, results were compared from normoxic and hypoxic conditions, they showed that Fucoidan had anti-angiogenic activity. Furthermore, in a multiple myeloma xenograft mouse model, it indicated that Fucoidan negatively affected tumor growth and angiogenesis in vivo. In conclusion, our results demonstrate that Fucoidan was able to interfere with angiogenesis of multiple myeloma cells both in vitro and in vivo and may have a substantial potential in the treatment of MM.

Gefitinib is a first tyrosine kinase inhibitor (TKI) designed with an EGFR tyrosine kinase for lung cancer targeted therapy. However, some lung cancer…

(2016). Fucoidan Induces Cell Aggregation and Apoptosis in Osteosarcoma MG-63 Cells. Animal Cells and Systems: Vol. 20, No. 4, pp. 186-192.

Background Cancer metastasis is the main cause leading to disease recurrence and high mortality in cancer patients. Therefore, inhibiting metastasis process or killing metastatic cancer cells by inducing apoptosis is of clinical importance in improving cancer patient survival. Previous studies revealed that fucoidan, a fucose-rich polysaccharide isolated from marine brown alga, is a promising natural product with significant anti-cancer activity. However, little is known about the role of endoplasmic reticulum (ER) stress in fucoidan-induced cell apoptosis. Principal Findings We reported that fucoidan treatment inhibits cell growth and induces apoptosis in cancer cells. Fucoidan treatments resulted in down-regulation of the glucose regulated protein 78 (GRP78) in the metastatic MDA-MB-231 breast cancer cells, and of the ER protein 29 (ERp29) in the metastatic HCT116 colon cancer cells. However, fucoidan treatment promoted ER Ca2+-dependent calmodulin-dependent kinase II (CaMKII) phosphorylation, Bcl-associated X protein (Bax) and caspase 12 expression in MDA-MB-231 cells, but not in HCT116 cells. In both types of cancer cells, fucoidan activated the phosphorylation of eukaryotic initiation factor 2 alpha (p-eIF2α)\CCAAT/enhancer binding protein homologous protein (CHOP) pro-apoptotic cascade and inhibited the phosphorylation of inositol-requiring kinase 1 (p-IRE-1)\X-box binding proteins 1 splicing (XBP-1s) pro-survival cascade. Furthermore, CHOP knockdown prevented DNA damage and cell death induced by fucoidan. Conclusion/Significance Fucoidan exerts its anti-tumor function by modulating ER stress cascades. Contribution of ER stress to the fucoidan-induced cell apoptosis augments our understanding of the molecular mechanisms underlying its anti-tumour activity and provides evidence for the therapeutic application of fucoidan in cancer.

Hyperlipidemia is associated with increased risk of the development of cardiovascular diseases. Although a great deal of attention has been paid to th…

The use of fucoidan, a marine-origin bioactive polymer, is herein proposed as a component of an innovative and effective strategy against melanoma, one of the most aggressive skin cancers. First, fucoidan antitumor activity, in its soluble form, was assessed presenting increased cytotoxicity over melanoma cells when compared to human dermal fibroblasts and keratinocytes. After this antitumor activity validation and trying to develop a more targeted and local strategy aiming to diminish the cytotoxic effects over noncancer cells, fucoidan was immobilized at the surface of an electrospun nanofiber mesh (NFM_Fu), envisioning the development of a therapeutic patch. The maximum immobilization concentration was 1.2 mg mL–1, determined by the Toluidine Blue Assay and confirmed by XPS. Furthermore, NFM_Fu is more hydrophilic than NFM, presenting a contact angle of 36°, lower than the 121° of the control condition. NFM_Fu was able to reduce human melanoma cell viability by 50% without affecting human dermal fibroblasts and keratinocytes. Taken together, these results set the basis for a valuable approach for melanoma treatment.

Lymphangiogenesis is one of the promoters of tumor lymphatic metastasis. Fucoidan which is a fucose-enriched sulfated polysaccharide has effect on various pharmacological activities including anti-metastasis activity. However, the inhibitory effect of fucoidan on lymphangiogenesis remains unclear. H …

Preclinical Research Fucoidan, a sulfated polysaccharide, is a compound found in various species of seaweed that has anti-viral, anti-bacterial, anti-oxidant, anti-inflammatory, and immuno...

Chronic kidney disease (CKD) is a significant risk factor for cardiovascular and peripheral vascular disease. Although mesenchymal stem cell (MSC)-bas…

Poor pancreatic cancer (PC) prognosis has been attributed to its resistance to apoptosis and propensity for early systemic dissemination. Existing the…

Cardiovascular diseases, including heart attacks and strokes, continue to be the leading causes of deaths resulting in more than 17 million deaths each year worldwide. Cardiovascular disease (CVD) man...

Fucoidan, a sulfated polysaccharide derived from brown seaweeds, has been shown to reduce blood glucose levels and improve insulin sensitivity in mice…

Fucoidan is a fucose-rich polysaccharide that has gained attention for its various anticancer properties. However, the effect and underlying mechanism…

Fucoidan is a marine-origin sulfated polysaccharide that can show anticancer activity, to which both pro- and anti-angiogenic responses have been repo…

Background Hepatitis B virus (HBV) infection is a serious public health problem leading to cirrhosis and hepatocellular carcinoma. As the clinical utility of current therapies is limited, the development of new therapeutic approaches for the prevention and treatment of HBV infection is imperative. Fucoidan is a natural sulfated polysaccharide that extracted from different species of brown seaweed, which was reported to exhibit various bioactivities. However, it remains unclear whether fucoidan influences HBV replication or not. Methods The HBV-infected mouse model was established by hydrodynamic injection of HBV replicative plasmid, and the mice were treated with saline or fucoidan respectively. Besides, we also tested the inhibitory effect of fucoidan against HBV infection in HBV-transfected cell lines. Results The result showed that fucoidan from Fucus vesiculosus decreased serum HBV DNA, HBsAg and HBeAg levels and hepatic HBcAg expression in HBV-infected mice. Moreover, fucoidan treatment also suppressed intracellular HBcAg expression and the secretion of the HBV DNA as well as HBsAg and HBeAg in HBV-expressing cells. Furthermore, we proved that the inhibitory activity by fucoidan was due to the activation of the extracellular signal-regulated kinase (ERK) pathway and the subsequent production of type I interferon. Using specific inhibitor of ERK pathway abrogated the fucoidan-mediated inhibition of HBV replication. Conclusion This study highlights that fucoidan might be served as an alternative therapeutic approach for the treatment of HBV infection.

Background/Aims: Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic effects. We aimed to study the effe

Despite major scientific advances in its prevention, treatment and care, hypertension remains a serious condition that might lead to long-term complications such as heart disease and stroke. The great majority of forms of hypertension eventually result from an increased vasomotor tone activity that is regula

What does clinical course of COVID-19 look like for both those who survive and those who don’t? How the symptoms compare to the flu. Subscribe to NutritionFacts.org’s free newsletter to receive our B12 infographic that covers the latest research takeaways and Dr. Greger’s updated recommendations: https://nutritionfacts.org/subscribe/ This is the 7th in a 17-video series on pandemics and COVID-19. If you’ve already seen these videos as part of my two webinars, or already watched the digital download (https://drgreger.org/collections/downloads/products/how-to-survive-a-pandemic-digital), keep your eyes out on Fridays as we continue our Flashback Friday series, and explore the many topics we have here on NutritionFacts.org (https://nutritionfacts.org/topics/). Here are the first six: · Where Do Deadly Coronaviruses Like MERS-CoV Come From? (http://nutritionfacts.org/video/where-do-deadly-coronaviruses-like-mers-cov-come-from) · The SARS Coronavirus and Wet Markets (http://nutritionfacts.org/video/the-sars-coronavirus-and-wet-markets) · Where Did the COVID-19 Coronavirus Come From? (http://nutritionfacts.org/video/where-did-the-covid-19-coronavirus-come-from) · The Last Coronavirus Pandemic May Have Been Caused by Livestock (http://nutritionfacts.org/video/the-last-coronavirus-pandemic-may-have-been-caused-by-livestock) · R0 and Incubation Periods: How Other Coronavirus Outbreaks Were Stopped (http://nutritionfacts.org/video/r0-and-incubation-periods-how-other-coronavirus-outbreaks-were-stopped) · Social Distancing, Lockdowns & Testing: How to Slow the COVID-19 Pandemic (http://nutritionfacts.org/video/social-distancing-lockdowns-testing-how-to-slow-the-covid-19-pandemic) Stay tuned for: · Modifiable Risk Factors and Comorbidities for Severe COVID-19 Infection (http://nutritionfacts.org/video/modifiable-risk-factors-and-comorbidities-for-severe-covid-19-infection) · The Immune System and COVID-19 Treatment (http://nutritionfacts.org/video/the-immune-system-and-covid-19-treatment) · Would Zinc Lozenges Help with COVID-19? (http://nutritionfacts.org/video/would-zinc-lozenges-help-with-covid-19) · How to Avoid COVID-19 (http://nutritionfacts.org/video/how-to-avoid-covid-19) · Hand Washing & Sanitizing to Inactivate COVID-19 Coronavirus (http://nutritionfacts.org/video/hand-washing-sanitizing-to-inactivate-covid-19-coronavirus) · What to Do if You Come Down with COVID-19 (http://nutritionfacts.org/video/what-to-do-if-you-come-down-with-covid-19) · The Best Mask or DIY Face Covering for COVID-19 (http://nutritionfacts.org/video/the-best-mask-or-diy-face-covering-for-covid-19) · How COVID-19 Ends: Vaccination, Mutations, and Herd Immunity (http://nutritionfacts.org/video/how-covid-19-ends-vaccination-mutations-and-herd-immunity) · The COVID-19 Pandemic May Just Be a Dress Rehearsal (http://nutritionfacts.org/video/the-covid-19-pandemic-may-just-be-a-dress-rehearsal) · How to Prevent the Next Pandemic (http://nutritionfacts.org/video/how-to-prevent-the-next-pandemic) You can download the whole series (for free) right now at https://drgreger.org/collections/downloads/products/how-to-survive-a-pandemic-digital and take an even deeper dive in my new book How to Survive a Pandemic (https://nutritionfacts.org/how-to-survive-a-pandemic/) (note: all my proceeds from this book are donated to pandemic prevention charities). Have a question about this video? Leave it in the comment section at http://nutritionfacts.org/video/covid-19-symptoms-vs-the-flu-a-cold-or-allergies and someone on the NutritionFacts.org team will try to answer it. Want to get a list of links to all the scientific sources used in this video? Click on Sources Cited at https://nutritionfacts.org/video/covid-19-symptoms-vs-the-flu-a-cold-or-allergies. You’ll also find a transcript and acknowledgements for the video, my blog and speaking tour schedule, and an easy way to search (by translated language even) through our videos spanning more than 2,000 health topics. Thanks for watching. I hope you’ll join in the evidence-based nutrition revolution! -Michael Greger, MD FACLM Captions for this video are available in several languages; you can find yours in the video settings. Image credit: annaj / pixabay https://NutritionFacts.org • Subscribe: https://nutritionfacts.org/subscribe • Donate: https://nutritionfacts.org/donate • How to Survive a Pandemic: https://nutritionfacts.org/how-to-survive-a-pandemic • How Not to Die: https://nutritionfacts.org/book • How Not to Diet: https://nutritionfacts.org/how-not-to-diet • Facebook: www.facebook.com/NutritionFacts.org

Click here to subscribe - https://www.glutenfreesociety.org/wxrn Coronavirus resource page: https://www.glutenfreesociety.org/viral-outbreaks ** The products, supplements, vitamins, minerals, herbs, etc mentioned in these videos and article are not intended to mitigate, prevent, treat, diagnose, or cure COVID-19 in people. There currently are no vaccines, pills, potions, lotions, lozenges or other prescription or over the-counter products available to treat or cure coronavirus disease 2019 (COVID-19) The information in these videos, and article is provided for educational purposes and should not be construed as medical advice. If you are seeking medical advice in regards to COVID-19 please visit the WHO website: https://www.who.int/emergencies/diseases/novel-coronavirus-2019/events-as-they-happen To connect with Dr. Osborne visit: On the web: https://drpeterosborne.com/ Facebook: https://www.facebook.com/DoctorPeterO... Pinterest: https://www.pinterest.com/docosborne/ Instagram: https://www.instagram.com/drosborne Twitter: https://twitter.com/glutenology *These statements have not been evaluated by the Food and Drug Administration. This video is not intended to diagnose, treat, cure or prevent any disease. It is strictly intended for educational purposes only. Additionally, this information is not intended to replace the advice of your physician. Dr. Osborne is not a medical doctor. He does not treat or diagnose disease. He offers nutritional support to people seeking an alternative from traditional medicine. Dr. Osborne is licensed with the Pastoral Medical Association.

Fucoidan from sea cucumber is reported to exhibit anti-hyperglycaemic effect, but its influence on pancreatic islets is lacking. The effects of fucoid …

Fucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method. In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S). The five products differed in molecular composition. The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans. The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S. Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines—MCF-7 and MDA-MB-231. Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines. A time-dependent inhibition was only observed in the MCF-7. Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway. LMWF stimulated a dose-dependent NOS activation in the MDA-MB-231 cells. In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines. Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.