Judicial Watch announced that it has filed a FOIA lawsuit against the DOD for records about House Speaker Nancy Pelosi’s January 8, 2021, telephone call with Chairman of the Joint Chiefs of Staff Mark Milley. READ: https://t.co/6jyyUA1OD1— Judicial Watch ⚖️ (@JudicialWatch) October 1, 2021
Frontiers | Ivermectin has New Application in Inhibiting Colorectal Cancer Cell Growth | Pharmacology
Colorectal cancer (CRC) is the third most common cancer worldwide and still lacks effective therapy. Ivermectin, an antiparasitic drug, has been shown to possess anti-inflammation, anti-virus, and antitumor properties. However, whether ivermectin affects CRC is still unclear. The objective of this study was to evaluate the influence of ivermectin on CRC using CRC cell lines SW480 and SW1116. We used CCK-8 assay to determine the cell viability, used an optical microscope to measure cell morphology, used Annexin V-FITC/7-AAD kit to determine cell apoptosis, used Caspase 3/7 Activity Apoptosis Assay Kit to evaluate Caspase 3/7 activity, used Western blot to determine apoptosis-associated protein expression, and used flow cytometry and fluorescence microscope to determine the reactive oxygen species (ROS) levels and cell cycle. The results demonstrated that ivermectin dose-dependently inhibited colorectal cancer SW480 and SW1116 cell growth, followed by promoting cell apoptosis and increasing Caspase-3/7 activity. Besides, ivermectin upregulated the expression of proapoptotic proteins Bax and cleaved PARP and downregulated antiapoptotic protein Bcl-2. Mechanism analysis showed that ivermectin promoted both total and mitochondrial ROS production in a dose-dependent manner, which could be eliminated by administering N-acetyl-l-cysteine (NAC) in CRC cells. Following NAC treatment, the inhibition of cell growth induced by ivermectin was reversed. Finally, ivermectin at low doses (...
Critics accuse ABC of selective editing in Obama interview
ABC News is being accused of selective editing following the release of an interview with former President Barack Obama. The recently aired interview on Good Morning America shows Obama scolding Republicans over immigration reform.
Biden's Vax Mandate To Be Enforced By Fining Companies $70,000 To $700,000?
Buried on page 168 of the House Democrats’ 2,465-page mega bill is a tenfold increase in fines for employers that “willfully,” “repeatedly,” or even seriously violate a section of labor law that deals with hazards, death, or serious physical harm to their employees.
Navy SEALs Told They're Undeployable If They Don't Get A COVID-19 Vaccine: Lawyers
“These guys are not anti-vax, they just - given the extraordinarily low risk of COVID to them and the substantial risk of unknown long-term effects of the vaccine - they aren’t comfortable with it right now,”
Millions Of Chinese Residents Lose Power After Widespread, "Unexpected" Blackouts; Power Company Warns This Is "New Normal"
One power company said it expected the power cuts to last until spring next year, and that unexpected outages would become "the new normal." Its post, however, was later deleted.
Fmr. Intel official bashes President Trump’s possible 2024 run, claims Trump spreads ‘disinformation’
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The audit of 2020 elections in Arizona found 49,000 votes raised questions of authenticity and legitimacy of election outcomes. According to the audit report, more than 23,000 votes came from people who no longer lived at the address where their mail-in ballot was sent.