GENE THEIVES & MAD ANTI-SCIENTISTS

Creative Proteomics can help you to determine the DNA methylation with various biochemical assays and mass spectrometry.

Arhivsko gradivo Povjernik za informiranje osigurava pravnu zaštitu u vezi dostupnosti javnog arhivskog gradiva pod uvjetima propisanim Zakonom o arhivskom gradivu i arhivima. Zakon o arhivskom gradivu i arhivima jamči pravo na pristup javnom arhivskom gradivu svim pravnim i fizičkim osobe pod jednakim uvjetima uvidom ili drugim oblicima korištenja sukladno odredbama tog

A compilation of frequently-asked questions about the Combined DNA Index System (CODIS) and the National DNA Index System (NDIS).

Human trafficking survivor Natasha Herzig tells stories of being kidnapped and forced into working as a prostitute, being moved around the country to service high paying clients and posing for porn. Herzig also talks of being made to call her family and tell them she was fine. Hear her story on part 2 of Global Perspectives look at human trafficking.

Chromosomal DNA comes in two flavors—euchromatin, which contains most of the expressed genes, and heterochromatin, which usually remains quiet. But what keeps genes within heterochromatin silent? Tchasovnikarova et al. examined the basis for this type of silencing in mammalian cells (see the Perspective by Brummelkamp). They identified a complex of proteins in human cells they called HUSH that kept particular parts of the genome silent by changing associated histone methylation marks. Science , this issue p. [1481][1], see also p. [1433][2] Forward genetic screens in Drosophila melanogaster for modifiers of position-effect variegation have revealed the basis of much of our understanding of heterochromatin. We took an analogous approach to identify genes required for epigenetic repression in human cells. A nonlethal forward genetic screen in near-haploid KBM7 cells identified the HUSH (human silencing hub) complex, comprising three poorly characterized proteins, TASOR, MPP8, and periphilin; this complex is absent from Drosophila but is conserved from fish to humans. Loss of HUSH components resulted in decreased H3K9me3 both at endogenous genomic loci and at retroviruses integrated into heterochromatin. Our results suggest that the HUSH complex is recruited to genomic loci rich in H3K9me3, where subsequent recruitment of the methyltransferase SETDB1 is required for further H3K9me3 deposition to maintain transcriptional silencing. [1]: /lookup/doi/10.1126/science.aaa7227 [2]: /lookup/doi/10.1126/science.aac6529

Nanoparticles releasing an oxaliplatin prodrug and a cationic DNA intercalator within temozolomide-resistant gliomas in mice after convection-enhanced delivery inhibit the growth of the tumours without causing any detectable toxicity.

Chromatin dynamics play a central role in maintaining genome integrity, but how this is achieved remains largely unknown. Here, we report that microrchidia CW-type zinc finger 2 (MORC2), an uncharacterized protein with a derived PHD finger domain and a conserved GHKL-type ATPase module, is a physiological substrate of p21-activated kinase 1 (PAK1), an important integrator of extracellular signals and nuclear processes. Following DNA damage, MORC2 is phosphorylated on serine 739 in a PAK1-dependent manner, and phosphorylated MORC2 regulates its DNA-dependent ATPase activity to facilitate chromatin remodeling. Moreover, MORC2 associates with chromatin and promotes gamma-H2AX induction in a PAK1 phosphorylation-dependent manner. Consequently, cells expressing MORC2-S739A mutation displayed a reduction in DNA repair efficiency and were hypersensitive to DNA-damaging agent. These findings suggest that the PAK1-MORC2 axis is critical for orchestrating the interplay between chromatin dynamics and the maintenance of genomic integrity through sequentially integrating multiple essential enzymatic processes.

MORC2 encodes an ATPase that plays a role in chromatin remodeling, DNA repair, and transcriptional regulation. Heterozygous variants in MORC2 have bee…

We have previously shown that blood global DNA methylation (DNAm) differs between postprandial state (PS) and fasting state (FS) and is associated with BMI and polyunsaturated fatty acid (PUFA) (negatively and positively, respectively) in 12 metabolically healthy adult Mexican men (AMM cohort) equal …

Origene. Antibodies, Recombinant proteins, ELISA kits, RNAi, cDNA clones, Antibody Array, Luminex kits. Reagents and instruments for immunology, cell biology and molecular biology.

CTRNA : Chlamydia is caused by the obligate intracellular bacterium Chlamydia trachomatis and is the most prevalent sexually transmitted bacterial infection (STI) in the United States.(1,2) In 2010, 1.3 million documented cases were reported to the Centers for Disease Control and Prevention (CDC).(2) Given that 3 out of 4 infected women and 1 out of 2 infected men will be asymptomatic initially, the actual prevalence of disease is thought to be much greater than reported. The organism causes genitourinary infections in women and men and may be associated with dysuria as well as vaginal, urethral, or rectal discharge. In women, complications include pelvic inflammatory disease, salpingitis, and infertility. Approximately 25% to 30% of women who develop acute salpingitis become infertile.(2) Complications among men are rare but include epididymitis and sterility. Rarely, genital chlamydial infection can cause arthritis with associated skin lesions and ocular inflammation (Reiter syndrome). C trachomatis can be transmitted from the mother during delivery and is associated with conjunctivitis and pneumonia in the newborn. Finally, C trachomatis may cause hepatitis and pharyngitis in adults.   Once detected, the infection is easily treated by a short course of antibiotic therapy. Annual chlamydia screening is now recommended for all sexually active women age 25 years and younger and for older women with risk factors for infection, such as a new sex partner or multiple sex partners. The CDC also recommends that all pregnant women be given a screening test for Chlamydia infection.(2) Repeat testing for test-of-cure is not recommended after treatment with a standard treatment regimen unless patient compliance is in question, reinfection is suspected, or the patient's symptoms persist. Repeat testing of pregnant women, 3 weeks after completion of therapy, is also recommended to ensure therapeutic cure.(2)   Culture was previously considered to be the gold standard test for diagnosis of C trachomatis infection.(2) However, organisms are labile in vitro and precise specimen collection, transportation, and processing conditions are required to maintain organism viability, which is necessary for successful culturing. In comparison, nucleic acid amplification testing (NAAT) provides superior sensitivity and specificity and is now the recommended method for diagnosis in most cases.(3-5) Immunoassays and non-amplification DNA tests are also available for C trachomatis detection, but these methods are significantly less sensitive and less specific than NAAT.(2)   Improved screening rates and increased sensitivity of NAAT testing have resulted in an increased number of accurately diagnosed cases.(2) Improved detection rates result from both the increased performance of the assay. Early identification of infection enables sexual partners to seek testing and/or treatment as soon as possible and reduces the risk of disease spread. Prompt treatment reduces the risk of infertility in women.

How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: * Peptide fragmentation and interpretation of product ion spectra * Basic polyacrylamide gel electrophoresis * Preparation of protein digests for sequencing experiments * Mass spectrometric analysis using capillary liquid chromatography * Techniques for protein identification by database searches * Characterization of modified peptides using tandem mass spectrometry And much more

Abstract. Identity by descent (IBD) can be reliably detected for long shared DNA segments, which are found in related individuals. However, many studies contain

DNA profiling plays a major role in sexual assault cases, but it has a significant shortcoming: Using the current standard DNA profiling method, many jurisdictions require the collection of evidentiary samples within three days of the assault. After that, it may become difficult to obtain a usable DNA profile of the male suspect. What if we could extend the window of time for collecting evidence?

It’s a sad month. The core foundation of genetic genealogy is sharing. GDPR is NOT about sharing easily, and the GDPR hoops are onerous, to be charitable. I wrote about GDPR in the articles GDPR – …

DNA fluorescence in situ hybridization (DNA FISH) has emerged as a powerful microscopy technique that allows a unique view into the composition and arrangement of the genetic material in its natural...

Hypersensitivity drug reactions (HDRs) encompass a wide spectrum of unpredictable clinical entities. They represent an important health problem, affecting people of all ages, and lead to a large strain on the public health system. Here, we summarize experiments that use high‐throughput genomics technologies to investigate HDRs. We also introduce the field of systems biology as a relatively recent discipline concerned with the integration and analysis of high‐throughput data sets such as DNA microarrays and next‐generation sequencing data. We describe previous studies that have applied systems biology techniques to related fields such as allergy and asthma. Finally, we present a number of potential applications of systems biology to the study of HDRs, in order to make the reader aware of the types of analyses that can be performed and the insights that can be gained through their application.

We use high-density single nucleotide polymorphism (SNP) genotyping microarrays to demonstrate the ability to accurately and robustly determine whether individuals are in a complex genomic DNA mixture. We first develop a theoretical framework for detecting an individual's presence within a mixture, …

The IMAGiNe Study (Immunoglobulin M(IgM)-Anti-myelin-associated-glycoprotein (MAG) Peripheral Neuropathy Study) aims to create a standardized database and biobank to be used for the research and analysis of Anti-MAG peripheral neuropathy. The goal is to be able to identify and predict the disease progression and treatment response, drawing on the information and DNA collected from the biobank and patient database. The hope is that researchers will be able to determine cause and treatments for sufferers of the disease. Anti-MAG peripheral neuropathy is a form of peripheral neuropathy caused by a rare autoimmune condition. With this type of peripheral neuropathy, a patient’s immune system attacks cells that are vital in maintaining a healthy peripheral nervous system. As these cells are destroyed by antibodies, they lose function and impact sensory and motor functions. More information on this condition can be found on the FPN website. Current members of the multinational consortium include UMC Utrecht (Netherlands), Johns Hopkins Hospital (USA), National Hospital for Neurology and Neurosurgery (UK), Hospital de la Santa Creu i Sant Pau (Spain), Clinical Center of Serbia (Serbia), Hopital de la Salpetriere (France), Humanitas Clinical Institute (Italy) and University Padova (Italy). FPN will be working closely with Johns Hopkins University as […]

PNRR Data Ongoing Projects | Summary Sheet A. Research Projects for which serum, plasma and/or DNA was provided together with data: 1.Biomarker Study / Johns Hopkins University (2016 – ongoing): The lipidomic component of the biomarker study identified a unique signature of changes in lipid levels in idiopathic peripheral neuropathy patients with pain versus no pain. This is currently being confirmed in a validation cohort (2019). If confirmed, this opens up the possibility of developing a new blood test to objectively measure pain level in patients with PN. This potentially could alter how we assess patients with neuropathic pain and how we treat them. The findings also point to new mechanisms of pain. JHU is working on exploring the feasibility of taking these observations from patient samples to mouse models of PN. The other components of the biomarker study were the examination of metabolites and proteins. No significant findings were observed in those evaluations. 2. Plumeria / Rutgers University (October 2018 – ongoing): Study on inflammation and neuropathic pain, specifically on Caucasian patients with diabetic neuropathy. 164 subjects (DNA, serum and plasma) were acquired from both painful DPN and non-painful DPN, both female and male. Study performed a genetic analysis […]

The Casework Direct System protocol entails a simple and rapid lysis of the sample, requiring no tube-to-tube transfers or DNA binding steps, producing a lysate compatible with RT-PCR quantification and PowerPlex STR amplification systems.

The Wizard® SV Genomic DNA Purification System provides a fast, membrane-based method for preparing genomic DNA from cultured cells and tissue, including mouse tails.

This DNA purification chapter addresses general information on the basics of DNA isolation, plasmid growth and DNA quantitation as well as how purification by silica can help increase your productivity so you spend less time purifying DNA and more time developing experiments and analyzing data.

Dr. Lutz Roewer is one of the renowned forensic scientists who transformed DNA forensics through studying the Y-chromosome and demonstrating the value of Y-STR analysis for forensic casework. I had the pleasure of interviewing Dr. Roewer while attending Promega’s 20th European Forensic DNA Working Group Meeting in Hamburg, Germany in November 2019.

Hunter Biden has been proved via DNA testing to be the father of Arkansas woman Lunden Alexis Roberts' baby, despite claims he never had sex with her.

Two cDNA clones encoding the pan-B cell CD20 antigen were isolated from a COS cell expression library. The two clones bear identical coding sequences and differ only in the length of the 3' untranslated region. The predicted CD20 sequence is 297 residues long and contains three hydrophobic domains, …

Two cDNA clones encoding the pan-B cell CD20 antigen were isolated from a COS cell expression library. The two clones bear identical coding sequences and differ only in the length of the 3' untranslated region. The predicted CD20 sequence is 297 residues long and contains three hydrophobic domains, …

The nucleotide sequence of a 1,091-base pair cloned cDNA insert encoding bovine corticotropin-beta-lipotropin precursor mRNA is reported. The corresponding amino acid sequence indicates that the precursor protein consists of repetitive units and includes a third melanotropin sequence in its cryptic …