GENE THEIVES & MAD ANTI-SCIENTISTS

We use high-density single nucleotide polymorphism (SNP) genotyping microarrays to demonstrate the ability to accurately and robustly determine whether individuals are in a complex genomic DNA mixture. We first develop a theoretical framework for detecting an individual's presence within a mixture, …

The IMAGiNe Study (Immunoglobulin M(IgM)-Anti-myelin-associated-glycoprotein (MAG) Peripheral Neuropathy Study) aims to create a standardized database and biobank to be used for the research and analysis of Anti-MAG peripheral neuropathy. The goal is to be able to identify and predict the disease progression and treatment response, drawing on the information and DNA collected from the biobank and patient database. The hope is that researchers will be able to determine cause and treatments for sufferers of the disease. Anti-MAG peripheral neuropathy is a form of peripheral neuropathy caused by a rare autoimmune condition. With this type of peripheral neuropathy, a patient’s immune system attacks cells that are vital in maintaining a healthy peripheral nervous system. As these cells are destroyed by antibodies, they lose function and impact sensory and motor functions. More information on this condition can be found on the FPN website. Current members of the multinational consortium include UMC Utrecht (Netherlands), Johns Hopkins Hospital (USA), National Hospital for Neurology and Neurosurgery (UK), Hospital de la Santa Creu i Sant Pau (Spain), Clinical Center of Serbia (Serbia), Hopital de la Salpetriere (France), Humanitas Clinical Institute (Italy) and University Padova (Italy). FPN will be working closely with Johns Hopkins University as […]

PNRR Data Ongoing Projects | Summary Sheet A. Research Projects for which serum, plasma and/or DNA was provided together with data: 1.Biomarker Study / Johns Hopkins University (2016 – ongoing): The lipidomic component of the biomarker study identified a unique signature of changes in lipid levels in idiopathic peripheral neuropathy patients with pain versus no pain. This is currently being confirmed in a validation cohort (2019). If confirmed, this opens up the possibility of developing a new blood test to objectively measure pain level in patients with PN. This potentially could alter how we assess patients with neuropathic pain and how we treat them. The findings also point to new mechanisms of pain. JHU is working on exploring the feasibility of taking these observations from patient samples to mouse models of PN. The other components of the biomarker study were the examination of metabolites and proteins. No significant findings were observed in those evaluations. 2. Plumeria / Rutgers University (October 2018 – ongoing): Study on inflammation and neuropathic pain, specifically on Caucasian patients with diabetic neuropathy. 164 subjects (DNA, serum and plasma) were acquired from both painful DPN and non-painful DPN, both female and male. Study performed a genetic analysis […]

Multi-modal nanomedicines that synergistically combine chemo-, gene-, and photothermal therapy have shown great potential for cancer treatment. In thi…

The Casework Direct System protocol entails a simple and rapid lysis of the sample, requiring no tube-to-tube transfers or DNA binding steps, producing a lysate compatible with RT-PCR quantification and PowerPlex STR amplification systems.

The Wizard® SV Genomic DNA Purification System provides a fast, membrane-based method for preparing genomic DNA from cultured cells and tissue, including mouse tails.

This DNA purification chapter addresses general information on the basics of DNA isolation, plasmid growth and DNA quantitation as well as how purification by silica can help increase your productivity so you spend less time purifying DNA and more time developing experiments and analyzing data.

Dr. Lutz Roewer is one of the renowned forensic scientists who transformed DNA forensics through studying the Y-chromosome and demonstrating the value of Y-STR analysis for forensic casework. I had the pleasure of interviewing Dr. Roewer while attending Promega’s 20th European Forensic DNA Working Group Meeting in Hamburg, Germany in November 2019.

Hunter Biden has been proved via DNA testing to be the father of Arkansas woman Lunden Alexis Roberts' baby, despite claims he never had sex with her.

Two cDNA clones encoding the pan-B cell CD20 antigen were isolated from a COS cell expression library. The two clones bear identical coding sequences and differ only in the length of the 3' untranslated region. The predicted CD20 sequence is 297 residues long and contains three hydrophobic domains, …

Two cDNA clones encoding the pan-B cell CD20 antigen were isolated from a COS cell expression library. The two clones bear identical coding sequences and differ only in the length of the 3' untranslated region. The predicted CD20 sequence is 297 residues long and contains three hydrophobic domains, …

The nucleotide sequence of a 1,091-base pair cloned cDNA insert encoding bovine corticotropin-beta-lipotropin precursor mRNA is reported. The corresponding amino acid sequence indicates that the precursor protein consists of repetitive units and includes a third melanotropin sequence in its cryptic …

We isolated and sequenced genomic and cDNA clones of the guinea pig preprodynorphin (ppDyn) mRNA. The sequence of ppDyn mRNA was deduced from a combination of genomic and cDNA clones: The primary structure of two coding exons was derived from a genomic clone and 5' and 3' untranslated sequences were …

Natera is a global leader in cell-free DNA (cfDNA) testing with a focus on women’s health, oncology, and organ health. Natera offers highly accurate solutions for noninvasive prenatal testing (NIPT), genetic-carrier screening, (PGD/PGS), and miscarriage testing. Learn more.

STEPS IN DNA ISOLATION

Scientists are still trying to make sense of something extremely weird that happened between a pair of Ambystoma salamanders millions of years ago.

DNA Isolation Methods Deoxyribonucleic acid (DNA ) isolation is an extraction process of DNA from various sources. Methods used to isolate DNA are dependent on the source, age, and size of the sample. Despite the wide variety of methods used, there are some similarities among them.

Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.

The Wizard™ MagneSil Plasmid DNA Purification System provides a simple, reliable method for rapid isolation of plasmid DNA in a 96-well, high-throughput format.

Origene. Antibodies, Recombinant proteins, ELISA kits, RNAi, cDNA clones, Antibody Array, Luminex kits. Reagents and instruments for immunology, cell biology and molecular biology.

Surname Projects for genealogical DNA testing