Site-directed mutagenesis by overlap extension using the polymerase chain reaction - ScienceDirect
Overlap extension represents a new approach to genetic engineering. Complementary oligodeoxyribo-nucleotide (oligo) primers and the polymerase chain r…
Directed/Produced by Kyrill Lazarov & Dario Nouri
(Update September 2019)
It is with great sadness we hear of the passing of Angelo Grubisic. Angelo died on August 21, 2019 while basejumping with his friends in Saudi Arabia. Our thoughts and prayers are with his family.
Client: ACE Climatic Wind Tunnel
Summary: We developed this short documentary to highlight the extreme testing capabilites of ACE. The ACE Climatic Wind Tunnel brought together physiology and aerodynamics experts from the University of Ontario Institute of Technology to help Angelo Grubisic break a wingsuit world record. This record will see Angelo jump from 40,000ft with a flight time of over 10 minutes and a top speed exceeding 250mph. Research took place in the climatic wind tunnel and included tests using a newly designed free flight fixture and tests with wind chill temperatures dropping below -100°C. This is a rare glimpse into the high-tech research that occurs at ACE.
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The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides: The araBAD promoter for tightly regulated expression Translation initiation signals opti
Summer Films: The Wiz - North Carolina Museum of Art
The Wiz (1978). Directed by Sidney Lumet, 135 min., G In this adaptation of the classic Wizard of Oz story, Dorothy (Diana Ross) is a shy Harlem kindergarten teacher who is transported to the…
Induction of the lac promoter in the absence of DNA loops and the stoichiometry of induction
Abstract. In vivo induction of the Escherichia coli lactose operon as a function of inducer concentration generates a sigmoidal curve, indicating a non-linear r
Teaser - A few days in the Dolomites testing a new prototype Icarus wingsuit. Full video coming soon. Eternal thanks to Squirrel for building our suits, Pressurized for load organising the helicopter.
Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages | PNAS
We report the identification and functional analysis of nine genes from Gram-positive and Gram-negative bacteria and their phages that are similar to lambda (λ) bet or Escherichia coli recT . Beta and RecT are single-strand DNA annealing proteins, referred to here as recombinases. Each of the nine other genes when expressed in E. coli carries out oligonucleotide-mediated recombination. To our knowledge, this is the first study showing single-strand recombinase activity from diverse bacteria. Similar to bet and recT , most of these other recombinases were found to be associated with putative exonuclease genes. Beta and RecT in conjunction with their cognate exonucleases carry out recombination of linear double-strand DNA. Among four of these foreign recombinase/exonuclease pairs tested for recombination with double-strand DNA, three had activity, albeit barely detectable. Thus, although these recombinases can function in E. coli to catalyze oligonucleotide recombination, the double-strand DNA recombination activities with their exonuclease partners were inefficient. This study also demonstrated that Gam, by inhibiting host RecBCD nuclease activity, helps to improve the efficiency of λ Red-mediated recombination with linear double-strand DNA, but Gam is not absolutely essential. Thus, in other bacterial species where Gam analogs have not been identified, double-strand DNA recombination may still work in the absence of a Gam-like function. We anticipate that at least some of the recombineering systems studied here will potentiate oligonucleotide and double-strand DNA-mediated recombineering in their native or related bacteria.
High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides | PNAS
Homologous DNA recombination is a fundamental, regenerative process within living organisms. However, in most organisms, homologous recombination is a rare event, requiring a complex set of reactions and extensive homology. We demonstrate in this paper that Beta protein of phage λ generates recombinants in chromosomal DNA by using synthetic single-stranded DNAs (ssDNA) as short as 30 bases long. This ssDNA recombination can be used to mutagenize or repair the chromosome with efficiencies that generate up to 6% recombinants among treated cells. Mechanistically, it appears that Beta protein, a Rad52-like protein, binds and anneals the ssDNA donor to a complementary single-strand near the DNA replication fork to generate the recombinant. This type of homologous recombination with ssDNA provides new avenues for studying and modifying genomes ranging from bacterial pathogens to eukaryotes. Beta protein and ssDNA may prove generally applicable for repairing DNA in many organisms. * oligo(s), : oligonucleotide(s); dsDNA, : double-stranded DNA; ssDNA, : single-stranded DNA
Started in 2012, the ICARUS research project aims to develop robotic tools which can assist “human” crisis intervention teams. This video provides for an overview of several ICARUS tools developped within the project since its beginning four years ago.
![[ICARUS] Legacy video - YouTube](https://rdl.ink/render/https%3A%2F%2Fi.ytimg.com%2Fvi%2FiwO7oesi2I4%2Fmqdefault.jpg?width=350&height=&ar=16:9&mode=crop&format=avif&dpr=2)
