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ABSTRACT To date, most genetic engineering approaches coupling the type II Streptococcus pyogenes clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system to lambda Red recombineering have involved minor single nucleotide mutations. ...

When I go on Google Drive and inspect the web page, I see a lot of tags. What are these? They seem to be used for my user specific content (what files I have). How does my browser know how to rend...

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Chromosome structure is a key regulator of gene expression. CTCF and cohesin play critical roles in structuring chromosomes by mediating physical inte…

Download scientific diagram | | The phototransduction cascade in vertebrate photoreceptors. (A) In the dark, 11-cis retinal is bound to rhodopsin, which is located inside the membrane of the outer segment's (OS) discs. The G-protein transducin (T) and phosphodiestherase (PDE) are inactive (upper panel). Cyclic guanosine monophosphate (cGMP) triggers the opening of cation channels in the photoreceptor membrane, which mediate a Na + influx (middle panel). This in turn depolarizes the membrane potential of the inner segment (IS) and triggers the release of glutamate (Glu) from ribbon synapses (lower panel). (B) Upon light absorption, 11-cis retinal is converted to all-trans retinal and dissociates from the rhodopsin. This activates the transducin, which in turn activates PDE, whose catalytic activity degrades cGMP (upper panel). This effectively lowers the cGMP concentration, which leads to closure of cation channels (middle panel). Thus, the IS is hyperpolarized and the Glu exocytosis is stopped (lower panel). Due to the G-protein involved in the transduction, a very high amplification of single photon responses is possible.   from publication: Biophysical Properties of Optogenetic Tools and Their Application for Vision Restoration Approaches | Optogenetics is the use of genetically encoded light-activated proteins to manipulate cells in a minimally invasive way using light. The most prominent example is channelrhodopsin2 (ChR2), which allows the activation of electrically excitable cells via light-dependent... | Optogenetics, Channelrhodopsin and Ion Pumps | ResearchGate, the professional network for scientists.

Engineered microbes are exciting alternatives to current diagnostics and therapeutics. Researchers have developed a wide range of genetic tools and parts to engineer probiotic and commensal microbes. Among these tools and parts, biosensors allow the microbes to sense and record or to sense and respo …

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