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Fighting Abuse @Scale brings together engineers, data scientists, product managers, operations specialists, and others fighting fraud, spam, and abuse on the internet at massive scale. At this one-day conference, practitioners will hear presentations on state-of-the art technologies used to protect people on the internet and have the opportunity to discuss abuse fighting with like-minded people […]

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 Forskere på KU-SUND har implementeret CRISPR/Cas9 mutations-teknologien i zebrafisk for at undersøge hvilken rolle generne spiller for en sund tarmflora .

Company profile page for Strategic Communication Laboratories Ltd including stock price, company news, executives, board members, and contact information

Communication in the Laboratory

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The Most Powerful Weapon In The World In a world where the perception is the reality, all countries need to have the capability to manage th...

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I was looking for a way to move my organization out of excel and into a visualization platform for dashboards and data. What I found in ClearPoint was much more.

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Background The MinION Access Program (MAP, 2014–2016) allowed selected users to test the prospects of long nanopore reads for diverse organisms and applications through the rapid development of improving chemistries. In 2014, faced with a fragmented Illumina assembly for the chloroplast genome of the green algal holobiont Caulerpa ashmeadii, we applied to the MAP to test the prospects of nanopore reads to investigate such intricacies, as well as further explore the hologenome of this species with native and hybrid approaches. Results The chloroplast genome could only be resolved as a circular molecule in nanopore assemblies, which also revealed structural variants (i.e. chloroplast polymorphism or heteroplasmy). Signal and Illumina polishing of nanopore-assembled organelle genomes (chloroplast and mitochondrion) reflected the importance of coverage on final quality and current limitations. In hybrid assembly, our modest nanopore data sets showed encouraging results to improve assembly length, contiguity, repeat content, and binning of the larger nuclear and bacterial genomes. Profiling of the holobiont with nanopore or Illumina data unveiled a dominant Rhodospirillaceae (Alphaproteobacteria) species among six putative endosymbionts. While very fragmented, the cumulative hybrid assembly length of C. ashmeadii’s nuclear genome reached 24.4 Mbp, including 2.1 Mbp in repeat, ranging closely with GenomeScope’s estimate (> 26.3 Mbp, including 4.8 Mbp in repeat). Conclusion Our findings relying on a very modest number of nanopore R9 reads as compared to current output with newer chemistries demonstrate the promising prospects of the technology for the assembly and profiling of an algal hologenome and resolution of structural variation. The discovery of polymorphic ‘chlorotypes’ in C. ashmeadii, most likely mediated by homing endonucleases and/or retrohoming by reverse transcriptases, represents the first report of chloroplast heteroplasmy in the siphonous green algae. Improving contiguity of C. ashmeadii’s nuclear and bacterial genomes will require deeper nanopore sequencing to greatly increase the coverage of these larger genomic compartments.

Develop a comprehensive 5-year strategic plan and present it accordingly to your target audience. Browse through different kinds of examples for references. Download a few 5-year strategic plan examples in PDF here.

Lawrence Berkeley National Laboratory is seeking a Digital Content Specialist to join the Strategic Communications Department, which shares and...