Research

The Arc is a powerful conceptual framework created by the Strozzi Institute that is a guide map to the journey of embodied change. It contains key distinctions that can help us recognise what stage our clients might be on that journey. As we are able to recognise this we can more skilfully and artfully support our clients to take the next step.

The future of construction is here. To build resiliency & future proof their businesses, leading AEC firms take their destiny into their hands.

Michael Meade introduces his new online series "The Arc of Transformation" that begins this Friday, December 4th and considers how myth and ritual touch and engage the entire person, spirit and mind, body and soul and make healing and radical transformation possible. What we learn from myth and ritual is how our human nature connects us to great nature and the mysteries of life, death and renewal. We look forward to you joining us for this new live series! INFO & REGISTRATION: mosaicvoices.org/events

At some point in time, deep and disruptive change will come a calling, be it for our civilizations, our societies, our cities, our institutions, our organizations, our systems, structures, processe…

We believe that individual, collective, and systemic transformation are interdependent—and while they can be furthered by themselves, they are uniquely powerful

The arc of transformation - a Visual Storytelling for Animation Projects course. In this lesson you will define an initial and final point for your protagonist, in addition to some (or some) antagonistic forces that help him achieve it.

As long as man has walked the Earth there has been an internal drive to evolve, a hunger for progress. From this craving the Illuminati were born. The secret society swore to protect and pursue the most impressive intellectual property of each generation, building upon it to perfect every new inv...

How to choose a geographic (datum) transformation.

ArcMap supports three types of spatial adjustment transformations: affine, similarity, and projective.

Empower facilities teams with digital transformation. Streamline plan access, save time, and enhance emergency preparedness. Read our inspiring stories.

The use of the arc-sine transformation in the analysis of variance is discussed. The application of the transformation can lead to difficult inference situation...

Hi all, Im 'trying to transform a component by a vector. Its work perfectly when they aren’t arc in the entities. On the following image, you can see the result. The initial vertex are represente by a cpoint the vector by the cline if I didn’t explode the arc , I have the following result: I try with entities.transform_by_vectors entities_to_move, vector and transform = Geom::Transformation.new vector entities.transform_entities(transform,entities_to_move.to_a) And I have the sa...

We describe a compact method to transform arc routing problem instances into node routing problem instances. Any node routing problem instance thus created must be solved by a branch-and-price process

We will transform the Australian Grains Industry from being commodity-based to being highly efficient and producing quality food and feed products.

ARC™ Scissors are the highest quality Japanese scissors, handcrafted by master smiths in Japan who are as passionate about making scissors as you are about cutting hair. When it comes to handcrafting scissors for professional hairstylists, ARC™ Scissors refuses to take any shortcuts—and that’s what sets ARC™ apart.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Background Pantoea ananatis, a member of the Enterobacteriacea family, is a new and promising subject for biotechnological research. Over recent years, impressive progress in its application to L-glutamate production has been achieved. Nevertheless, genetic and biotechnological studies of Pantoea ananatis have been impeded because of the absence of genetic tools for rapid construction of direct mutations in this bacterium. The λ Red-recombineering technique previously developed in E. coli and used for gene inactivation in several other bacteria is a high-performance tool for rapid construction of precise genome modifications. Results In this study, the expression of λ Red genes in P. ananatis was found to be highly toxic. A screening was performed to select mutants of P. ananatis that were resistant to the toxic affects of λ Red. A mutant strain, SC17(0) was identified that grew well under conditions of simultaneous expression of λ gam, bet, and exo genes. Using this strain, procedures for fast introduction of multiple rearrangements to the Pantoea ananatis genome based on the λ Red-dependent integration of the PCR-generated DNA fragments with as short as 40 bp flanking homologies have been demonstrated. Conclusion The λ Red-recombineering technology was successfully used for rapid generation of chromosomal modifications in the specially selected P. ananatis recipient strain. The procedure of electro-transformation with chromosomal DNA has been developed for transfer of the marked mutation between different P. ananatis strains. Combination of these techniques with λ Int/Xis-dependent excision of selective markers significantly accelerates basic research and construction of producing strains.