C. difficile | Meridian Bioscience
The future of Clostridioides difficile diagnostics
As rates of C. difficile infection decline, emphasis is now on improving test utilization and a quest for improved diagnostic approaches. These approaches may involve implementation of technologies that improve toxin testing, predict patients likely to have disease and/or a severe outcome, and harne …
New PCR Test Accurate for Detecting C Difficile, Norovirus - MD Magazine
The new assay was 100% on both sensitivity and specificity for detecting C difficile infections from rectal swabs.
Clostridioides (Clostridium) difficile Infection in Adults
Diagnosing Patients With Inflammatory Bowel Disease - MD Magazine
Panel of gastroenterologists share their perspectives regarding the diagnostic work-up for patients who may have symptoms suggestive of IBD.
Pragmatic Strategy for Fecal Specimen Storage and the Corresponding Test Methods for Clostridioides difficile Diagnosis
The quality of fecal specimens is one of the factors responsible for successful Clostridioides difficile infection (CDI) diagnosis. The quality depends largely on the storage conditions, including the temperature and time period. In this study, we organized the outputs of previous studies, fi …
First Light Diagnostics C. Difficile Test Nabs FDA Clearance - 360Dx
The test runs on the firm's MultiPath Analyzer for single molecule counting to detect C. difficile toxins A and B.
DICON on Twitter
@IbukunMD @JessieSeidelman @beckyasmith79 and team in pursuit of the holy grail -- improving #cdiff testing. pic.twitter.com/Lz98EIHsf1— DICON (@DICON_duke) June 8, 2021
Diagnosis of Clostridioides difficile infection by analysis of volatile organic compounds in breath, plasma, and stool: A cross-sectional proof-of-concept study
Clostridioides difficile infection (CDI) is an important infectious cause of antibiotic-associated diarrhea, with significant morbidity and mortality. Current diagnostic algorithms are based on identifying toxin by enzyme immunoassay (EIA) and toxin gene by real-time polymerase chain reaction (PCR) …
First Light Diagnostics receives FDA clearance for Clostridiodes difficile test
Diagnostic deficiencies of C. difficile infection among patients in a tertiary hospital in Saudi Arabia: A laboratory-based case series
Prevalence of detection of Clostridioides difficile among asymptomatic children: A systematic review and meta-analysis - MD Linx
New discovery provides insight into diagnostics and treatment for severe C. difficile infection | VCH Research Institute
Clostridioides difficile (C. diff) is the leading cause of gastroenteritis-associated death in North America, and infects around half a million Americans annually. While most people affected will
Association between Clostridioides difficile infection testing results and decision to treat
We sought to determine how often patients with a negative toxin enzyme immunoassay following a positive nucleic acid amplification test for Clostridioides difficile infection (CDI) were treated for CDI in Veterans Affairs facilities. From October 2018 through March 2021, 702 (29.5%) of 2,374 unique …
Philippe Cleuziat: 30 Years of In Vitro Diagnostics - Combacte
Development and clinical validation of an automated cell cytotoxicity neutralization assay for detecting Clostridioides difficile toxins in clinically relevant stools samples
Overall, the automated CCNA provides a specific, sensitive, and reproducible tool to support determination of CDI epidemiology or the efficacy of interventions such as vaccines.
First Light Diagnostics Receives FDA Clearance for its Rapid Clostridiodes difficile Test on the MultiPath® Analyzer - Yahoo Finance
First Light Diagnostics is developing and commercializing breakthrough automated diagnostic products for rapidly detecting infections and determining optimal treatment. The company announced today that the US Food and Drug Administration (FDA) has cleared its SensiTox™ C. difficile Toxin Test along with its proprietary MultiPath Analyzer to be marketed in the US.
Genomic Analysis of Clostridioides difficile in 2 Regions of the United States Reveals a Diversity... Whole-genome sequencing of 422 Clostridioides difficile strains from healthcare and community settings across 2 sites over a 3-month period demonstrated a diver academic.oup.com
CDI Risk Factors Identified for Geriatric Patients Renal failure, anemia, malnutrition and the presence of infection at the time of hospital admission are all risk factors for the development of Clostridioides difficile infection in older patients. idse.net
First Light Diagnostics Receives FDA Clearance for its Rapid Clostridiodes difficile Test on the MultiPath® Analyzer - KPVI News 6
Featured CE: Payer Expert James Kenney, RPh on C. Diff Costs
Calprotectin Stool Test: Uses, Procedure, Results & More - Healthline
The calprotectin stool test is a noninvasive test that can help determine if you have an inflammatory or noninflammatory bowel condition. Learn more about what this test measures and when it's used.
Using a nurse-initiated bedside tool to decrease inappropriate testing for Clostridioides difficile in hospital settings
Overdiagnosis of Clostridioides difficile (C. difficile) is associated with increased hospital length of stay, antibiotic overuse, unnecessary infection prevention efforts and excess costs. This study evaluated a paper-based bedside C. difficile screening tool on the number of …
Reductions in positive Clostridioides difficile events reportable to National Healthcare Safety Network (NHSN) with adoption of reflex enzyme immunoassay (EIA) testing in 13 Atlanta hospitals
Performance of commercial PCR assays to detect toxigenic Clostridioides difficile in the feces of puppies
Changes In Electronic Health Records (EHR) To Guide Clinicians In C. diff. Diagnostic Stewardship – To Pause Testing When Not Clinically Indicated | C Diff Foundation
An intervention that required administrative approval of all Clostridioides difficile testing after hospital day 3 out-performed electronic health record-based support in reducing C. difficile test…
Test restrictions more effective than EHR-based support at reducing C. difficile testing - Healio
An intervention that required administrative approval of all Clostridioides difficile testing after hospital day 3 out-performed electronic health record-based support in reducing C. difficile testing, according to a study.“We performed this work as part of a larger quality improvement initiative with the goal of improving the accuracy of diagnosis of C. difficile infection in order to
Evaluation of loop-mediated isothermal amplification assay for detection of Clostridioides difficile infection: A prospective diagnostic performance study
A novel Loop-mediated isothermal amplification (LAMP) assay, HiberGene's CD was evaluated with 82 unformed stools from patients suspected of C. difficile infection (CDI). Compared to glutamate dehydrogenase (GDH) toxins A/B test (C.diff Quik Chek®), HiberGene's LAMP showed 100% of sensitivity and 95 …
Detection of Clostridioides difficile in hospital environment by using C diff Banana Broth
116 environmental samples from a 504 bed clinical hospital obtained in 2017/19 were inoculated into C diff Banana Broth™. Six C. difficile and 12 C. pefringens strains were isolated. Antibiotic-resistant Clostridium spp. dominated in hospital environment. To determine Clostridium spp. in hospital en …
Optimization of an Assay To Determine Colonization Resistance to Clostridioides difficile in Fecal Samples from Healthy Subjects and Those Treated with Antibiotics | Antimicrobial Agents and Chemotherapy
A healthy, intact gut microbiota is often resistant to colonization by gastrointestinal pathogens. During periods of dysbiosis, however, organisms such as Clostridioides difficile can thrive. We describe an optimized in vitro colonization resistance assay for C. difficile in stool (CRACS) and demonstrate the utility of this assay by assessing changes in colonization resistance following antibiotic exposure. Fecal samples were obtained from healthy volunteers (n = 6) and from healthy subjects receiving 5 days of moxifloxacin (n = 11) or no antibiotics (n = 10). Samples were separated and either not manipulated (raw) or sterilized (autoclaved or filtered) prior to inoculation with C. difficile ribotype 027 spores and anaerobic incubation for 72 h. Different methods of storing fecal samples were also investigated in order to optimize the CRACS. In healthy, raw fecal samples, incubation with spores did not lead to increased C. difficile total viable counts (TVCs) or cytotoxin detection. In contrast, increased C. difficile TVCs and cytotoxin detection occurred in sterilized healthy fecal samples or those from antibiotic-treated individuals. The CRACS was functional with fecal samples stored at either 4°C or −80°C but not with those stored with glycerol (12% or 30% [vol/vol]). Our data show that the CRACS successfully models in vitro the loss of colonization resistance and subsequent C. difficile proliferation and toxin production. The CRACS could be used as a proxy for C. difficile infection in clinical studies or to determine if an individual is at risk of developing C. difficile infection or other potential infections occurring due to a loss of colonization resistance.