C Diff Molecular

Editorial: Microbial interactions of Clostridioides difficile

Higher BMI and exposure to antibiotics are risk factors for C. difficile asymptomatic bacterial colonization in kids with cystic fibrosis.

Microbiome-based therapeutics are increasingly evaluated as a strategy to reduce recurrent Clostridioides difficile infection (rCDI), with proposed mechanisms including restoration of the microbiota and microbiota-mediated functions, such as bile acid (BA) metabolism. This study reports a qua …

Our study revealed the prevalence of CDI in COVID-19 patients was 0.65%. Although the prevalence was low, its presence is associated with worse outcomes and higher resource utilization.

CDT compromised epithelial barrier function in a human intestinal colonic cell model, even in sublethal concentrations, pointing to barrier dysfunction in the intestine and leak flux induction as a diarrheal mechanism. However, this cannot be attributed to the appearance of apoptosis and necrosis, b …

Clinical isolates of Clostridioides difficile sometimes exhibit multidrug resistance and cause diarrhea after antibiotic administration. Metronidazole and vancomycin are often used as therapeutic agents, but resistance to these antibiotics has been found clinically. Therefore, the development of alt …

@Maribeth_MD challenging #cdiff dogma: #cdiff toxin positivity does not distinguish patients with c diff disease vs colonization amongst pediatric patients with positive NAAT test, including in #IBD. #CCCongress23 pic.twitter.com/ph71GDBCZQ— Michael J Rosen, MD, MSCI (@DrRosenIBD) January 19, 2023

The main goals of this Research Topic are the following:• Assess CD underdiagnosis and the reasons for underdiagnosis, which can lead to a delay in prescribing adequate therapy and consequently may cause a higher complication rate. In addition, underdiagnosis may contribute to an increase in the CDI transmission within hospitals due to the missed or delayed application of infection prevention and control measures, including patient isolation.• Assess the best treatment for primary and recurrent CD infections, including severe, complicated and fulminant manifestations• Assess the role of antimicrobial stewardship and infection control to reduce the risk of CD acquisition in the hospital settings• Assess the role of microbiota/microbiome in CD infection• Antibiotics and CD infectionThe scope of this Research Topic is to collect manuscripts on the epidemiology of CD infection, risk factors for CD infect...

#DYK C. difficile bacteria may reside in a healthy gut? #WednesdayWisdom #guthealth #Cdiff pic.twitter.com/Tt5NhQXjPj— Microbiome Science (@MbiomeScience) January 18, 2023

Baseline stool toxin concentration is associated with risk of recurrence in children with Clostridioides difficile infection

Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition that often goes underdiagnosed because of broad and non-specific symptomatology, usually consisting of fever, hepatosplen...

The addition of probiotics did not reduce the incidence of HO-CDI among inpatients receiving IV antibiotics.

Researchers have shown that a group of antibiotic-resistant pathogens found in the gut—called enterococci—can make C. difficile more potent and dangerous.

The objective of this study is to determine the trend and diversity of binary toxin-positive Clostridioides difficile over 10 years in Korea. Binary toxin-positive strains were selected from a tertiary hospital in Korea in 2009-2018. The multi-locus sequence typing and antibiotic susceptibility test …

Clostridioide difficile is the leading cause of diarrhea disease worldwide and is a CDC-designated urgent threat level pathogen. Mammalian models are commonly utilized as gold standard to study the pathogenesis of C. difficile infection (CDI); however, alternatives are needed due to cost, higher thr …

Higher baseline stool toxin concentrations are present in children with CDI recurrence. Toxin quantification should be included in CDI treatment trials to evaluate its use in severity assessment and outcome prediction.

Maternal dietary factors have been reported to influence Clostridioides difficile colonisation in the offspring. Twenty suckling piglets from sows fed diets supplemented with high-fermentable sugar beet pulp (SBP) or low-fermentable lignocellulose (LNC) fibres during gestation and lactation were dis …

The three MLST Clade 2 isolated from different rybotipes, two of which were isolated from Latin America, are competent biofilm-forming bacteria, indicating their ability to induce C. difficile infection recurrence, making treatment difficult.

Clostridioides difficile (C. difficile) is an opportunistic pathogen that leads to antibiotic-associated diarrhoea and is a leading cause of morbidity and mortality worldwide. Antibiotic usage is the main risk factor leading to C. difficile infection (CDI), as a dysbiotic gut environment allows colo …

Nature Reviews Microbiology - In this Review, Caballero-Flores, Pickard and Núñez explore how the microbiota limits colonization by pathogens and the strategies used by pathogens to...

Clostridioides difficile (C. difficile) is an opportunistic pathogen that leads to antibiotic-associated diarrhoea and is a leading cause of morbidity and mortality worldwide. Antibiotic usage is the main risk factor leading to C. difficile infection (CDI), as a dysbiotic gut environment allows colo …

Clostridioides difficile is an opportunistic spore-forming pathogen responsible for antibiotic-associated diarrhea in humans. C. difficile produces two main toxins: TcdA and TcdB as well as a third toxin named binary toxin (CDT) that is also involved in virulence. The present study aimed at characte …

Clinical Microbiomics. Heidi Hau, PhD, leads business and scientific activities in the US including development strategies, external partnerships and CRO organi...

New research from the University of Virginia School of Medicine and collaborators shows that microorganisms found in our guts can worsen dangerous Clostridioides difficile infections, a discovery that could help doctors identify patients at risk for

Clostridioides difficile (Clostridium difficile) infection (CDI) is one of the main public health concerns in adults, while children under 2 years of age are often colonized asymptomatically. In both adults and children, CDI is strongly associated with disturbances in gut microbiota. In this …

C. difficile-targeted antibiotics disrupt the #microbiome, which can produce a favorable environment for the unaffected dormant #Cdiff spores to germinate and grow. #MicrobiomeMonday pic.twitter.com/L08ltfiVjt— Microbiome Science (@MbiomeScience) January 2, 2023

The gut mycobiome (fungi) is a small but crucial component of the gut microbiome in humans. Intestinal fungi regulate host homoeostasis, pathophysiological and physiological processes, and the assembly of the co-residing gut bacterial microbiome. Over the past decade, accumulating studies have characterised the gut mycobiome in health and several pathological conditions. We review the compositional and functional diversity of the gut mycobiome in healthy populations from birth to adulthood. We describe factors influencing the gut mycobiome and the roles of intestinal fungi—especially Candida and Saccharomyces spp—in diseases and therapies with a particular focus on their synergism with the gut bacterial microbiome and host immunity.

The results show similar rates o colonized C difficile among pediatric patients and adult patients with cystic fibrosis.

We report a patient case with pseudomembranous colitis associated with a mono-toxin producing Clostridioides difficile belonging to the very rarely di…

In summary: we confirmed plasmid co-carriage in a single isolate, confirmed two new replicons, and added a new complete genome #cdiff to GBK. Thanks to @EicJrood for handling our submission and the anonymous reviewers for comments that improved the work! (11/11)— Wiep Klaas Smits (@SmitsLab) December 27, 2022