Personalized Clostridioides difficile engraftment risk prediction and probiotic therapy assessment in the human gut - PubMed
Clostridioides difficile colonizes up to 30-40% of community-dwelling adults without causing disease 1, 2 . C. difficile infections (CDIs) are the leading cause of antibiotic-associated diarrhea in the U.S. 3, 4 and typically develop in individuals following disru …
Butyrate enhances Clostridioides difficile sporulation in vitro - PubMed
Several studies suggest that butyrate may be important in alleviating gut infections, such as reducing inflammation caused by the healthcare-associated Clostridioides difficile . While studies in both animal models and human studies correlate high levels of butyrate with reduced C. diffici …
[The fliL gene significantly affects the motility and sporulation abilities of Clostridioides difficile] - PubMed
Flagella are the main motility structure of Clostridioides difficile that affects the adhesion, colonization, and virulence of C. difficile in the human gastrointestinal tract. The FliL protein is a single transmembrane protein bound to the flagellar matrix. This study aimed to investi …
Excellent talk at #DDW2023 by Dr. @CTropini on creating a healthy gut microbiome environment. You wouldn’t “plant trees during a forest fire”. We need to create the gut environment (e.g pH, osmolality) to increase survival of #FMT treatment. @getgutsycanada pic.twitter.com/CNfspRloX4— Kate Lee (@KateLeeIBD) May 9, 2023
Re-evaluation of FDA-approved antibiotics with increased diagnostic accuracy for assessment of antimicrobial resistance
Heithoff et al. observe that antibiotic testing in cell culture medium improves the
accuracy by which laboratory testing predicts clinical outcomes in mice. Test methods
with increased diagnostic accuracy will address the antimicrobial resistance crisis
by improving the way antibiotics are developed, tested, and prescribed.
[The fliL gene significantly affects the motility and sporulation abilities of Clostridioides difficile] - PubMed
Flagella are the main motility structure of Clostridioides difficile that affects the adhesion, colonization, and virulence of C. difficile in the human gastrointestinal tract. The FliL protein is a single transmembrane protein bound to the flagellar matrix. This study aimed to investi …
Dormant Crohn's Disease Reactivated by Clostridioides difficile Infection - PubMed
Crohn's disease (CD) is a type of inflammatory bowel disease (IBD) characterized by chronic transmural inflammation of any portion of the gastrointestinal tract. The etiology of CD remains unknown although genetic, immunological, and acquired factors have been recognized as contributing to its devel …
Does gut microbiota really influence health and life expectancy? Here's why all the hype could be just that
The intestinal microbiota is the set of bacteria and viruses that live inside your gut. Microbiota perform a variety of functions, including digesting food and protecting against specific pathogens.
[Study on the features of Clostridioides difficile infection among diarrhea patients in Kunming from 2018 to 2020] - PubMed
Objective: We analyze the characteristics of Clostridioides difficile (C. difficile) infection among diarrhea patients in Kunming from 2018 to 2020 and provide evidence for follow-up surveillance and prevention. Methods: A total of 388 fecal samples of diarrhea patients f …
The Gut Microbiome Conference has become a successful event for keeping healthcare professionals and scientists informed about the latest advances in human microbiome science. Now in its 4th year, this one-of-a-kind platform provides attendees the opportunity to interact with the leading authorities on gut microbiome r
Checkpoint Inhibitor Colitis With Superimposed Clostridioides difficile Infection - PubMed
Immune checkpoint inhibitors (ICI) are commonly used for various malignancies. A particular checkpoint inhibitor is the anti-PD-1 antibody pembrolizumab. Immune-mediated diarrhea and colitis (IMDC) is the most frequently observed immune-related adverse event (irAE) involving the gastrointestinal sys …
A network of small RNAs regulates sporulation initiation in Clostridioides difficile - PubMed
The obligate anaerobic, enteric pathogen Clostridioides difficile persists in the intestinal tract by forming antibiotic-resistant endospores that contribute to relapsing and recurrent infections. Despite the importance of sporulation for C. difficile pathogenesis, environmental cues and molecular m …
Intestinal bile acids provide a surmountable barrier against C. difficile TcdB-induced disease pathogenesis - PubMed
Intestinal bile acids play an essential role in the Clostridioides difficile lifecycle having been shown in vitro to modulate various aspects of pathogenesis, including spore germination, vegetative growth, and more recently the action of the primary virulence determinant, TcdB. Here, we inve …
Fulminant Clostridioides difficile Colitis With SARS-CoV-2 Infection
Clostridioides difficile (C. difficile) and coronavirus disease 2019 (COVID-19) infections can have overlapping symptoms. Recently, the association and outcomes of coinfection have been studied....
The vanRCd Mutation 343AG, Resulting in a Thr115Ala Substitution, Is Associated with an Elevated Minimum Inhibitory Concentration (MIC) of Vancomycin in Clostridioides difficile Clinical Isolates from Florida - PubMed
Clostridioides difficile, the primary cause of nosocomial antibiotic-associated diarrhea, has a complex relationship with antibiotics. While the use of broad-spectrum antibiotics disrupts the gut microbiota and increases the risk of C. difficile infection (CDI), antibiotics are also the primary trea …
Coexisting cytomegalovirus colitis in an immunocompetent patient with Clostridioides difficile colitis: A case report - PubMed
Co-existing CMV colitis should be considered in patients with aggravated C. difficile infection on appropriate treatment, even in immunocompetent hosts.
Characterization of the gut microbiome of patients with Clostridioides difficile infection, patients with non- C. difficile diarrhea, and C. difficile-colonized patients - PubMed
We observed differences in microbiome patterns between healthy individuals, colonized patients, CDI, R-CDI, and NOCDI diarrhea. We identified possible microbiome biomarkers that could prove useful in the diagnosis of true CDI infections. Further studies are warranted.
Coexisting cytomegalovirus colitis in an immunocompetent patient with Clostridioides difficile colitis: A case report - PubMed
Co-existing CMV colitis should be considered in patients with aggravated C. difficile infection on appropriate treatment, even in immunocompetent hosts.
Characterization of the gut microbiome of patients with Clostridioides difficile infection, patients with non- C. difficile diarrhea, and C. difficile-colonized patients - PubMed
We observed differences in microbiome patterns between healthy individuals, colonized patients, CDI, R-CDI, and NOCDI diarrhea. We identified possible microbiome biomarkers that could prove useful in the diagnosis of true CDI infections. Further studies are warranted.
Effect of peripartum Clostridioides difficile infection on pregnancy and neonatal outcomes: an observational study - PubMed
Peripartum CDI was associated with higher odds of cesarean delivery and lower odds of GBS infections. Larger studies exploring the effect of CDI on pregnancy and neonatal outcomes are needed.
How dormant bacteria come back to life: Research provides answers to long-standing mystery of bacterial spores, illuminating new paths for disease prevention
Bacterial spores can survive for years, even centuries, without nutrients, resisting heat, UV radiation, and antibiotics. How inert, sleeping bacteria -- or spores -- spring back to life has been a century-long mystery. New research identifies how sensor proteins revive dormant bacteria. Discovery opens new routes to combat spore resistance to antibiotics and sterilization. Findings can inform novel strategies to prevent infections, food spoilage.
Metabolism from the magic angle - Nature Chemical Biology
Anaerobes have developed unique metabolic pathways and strategies, some of which are still not well understood. Now, a study that integrates spectroscopy with dynamic metabolic modeling reveals the metabolism of the anaerobic pathogen Clostridioides difficile.
Clostridioides difficile, a New "Superbug" - PubMed
Clostridioides difficile is a Gram-positive, spore-forming, anaerobic bacterium. The clinical features of C. difficile infections (CDIs) can vary, ranging from the asymptomatic carriage and mild self-limiting diarrhoea to severe and sometimes fatal pseudomembranous colitis. C. diffi …
Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells - PubMed
Clostridioides difficile infection (CDI) appears to be associated with different liver diseases. C. difficile secretes membrane vesicles (MVs), which may be involved in the development of nonalcoholic fatty liver disease (NALFD) and drug-induced liver injury (DILI). In this study, we i …