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Complete Genome Sequences of Evolutionary Clade C-III Strains of Clostridioides ( Clostridium) difficile Isolated from the Environment in Western Australia - PubMed
Complete Genome Sequences of Evolutionary Clade C-III Strains of Clostridioides ( Clostridium) difficile Isolated from the Environment in Western Australia - PubMed
Clostridioides (Clostridium) difficile in the environment is thought to contribute to C. difficile infection in community settings. Here, we provide complete genome assemblies for two esculin hydrolysis-negative strains of C. difficile that were isolated from soils in Western Australia; the strains …
·pubmed.ncbi.nlm.nih.gov·
Complete Genome Sequences of Evolutionary Clade C-III Strains of Clostridioides ( Clostridium) difficile Isolated from the Environment in Western Australia - PubMed
Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection - PubMed
Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection - PubMed
The anaerobic pathogen Clostridioides difficile, which is a primary cause of antibiotic-associated diarrhea, faces a variety of stresses in the environment and in the mammalian gut. To cope with these stresses, alternative sigma factor B (σB) is employed to modulate gene transcription, an …
·pubmed.ncbi.nlm.nih.gov·
Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection - PubMed
Network analysis of toxin production in Clostridioides difficile identifies key metabolic dependencies - PubMed
Network analysis of toxin production in Clostridioides difficile identifies key metabolic dependencies - PubMed
Clostridioides difficile pathogenesis is mediated through its two toxin proteins, TcdA and TcdB, which induce intestinal epithelial cell death and inflammation. It is possible to alter C. difficile toxin production by changing various metabolite concentrations within the extracellular environment. H …
·pubmed.ncbi.nlm.nih.gov·
Network analysis of toxin production in Clostridioides difficile identifies key metabolic dependencies - PubMed
Climate Change Could Trigger a Rise in Cases of C. Difficile
Climate Change Could Trigger a Rise in Cases of C. Difficile
Some research has shown an association between flooding, which is becoming more common with climate change, and Clostridioides difficile infections.
·managedhealthcareexecutive.com·
Climate Change Could Trigger a Rise in Cases of C. Difficile
How Dormant Bacteria Return to Life
How Dormant Bacteria Return to Life
Solution to long-standing mystery of bacterial spores illuminates new paths for disease prevention
·hms.harvard.edu·
How Dormant Bacteria Return to Life
In Vitro Selection and Characterization of a DNAzyme Probe for Diverse Pathogenic Strains of Clostridium difficile - PubMed
In Vitro Selection and Characterization of a DNAzyme Probe for Diverse Pathogenic Strains of Clostridium difficile - PubMed
Clostridium difficile frequently causes an infectious disease known as Clostridium difficile infection (CDI) and there are urgent needs for the development of more effective rapid diagnostic tests for CDI. Previously we have developed an RNA-cleaving fluorogenic DNAzyme (RFD) probe named RFD-CD1, wh …
·pubmed.ncbi.nlm.nih.gov·
In Vitro Selection and Characterization of a DNAzyme Probe for Diverse Pathogenic Strains of Clostridium difficile - PubMed
Dormant Crohn's Disease Reactivated by Clostridioides difficile Infection
Dormant Crohn's Disease Reactivated by Clostridioides difficile Infection
Crohn’s disease (CD) is a type of inflammatory bowel disease (IBD) characterized by chronic transmural inflammation of any portion of the gastrointestinal tract. The etiology of CD remains unkno...
·news.google.com·
Dormant Crohn's Disease Reactivated by Clostridioides difficile Infection
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase - PubMed
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase - PubMed
S-Adenosyl-l-methionine (SAM) analogs are adaptable tools for studying and therapeutically inhibiting SAM-dependent methyltransferases (MTases). Some MTases play significant roles in host-pathogen interactions, one of which is Clostridioides difficile-specific DNA adenine MTase (CamA). …
·pubmed.ncbi.nlm.nih.gov·
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase - PubMed
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase - PubMed
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase - PubMed
S-Adenosyl-l-methionine (SAM) analogs are adaptable tools for studying and therapeutically inhibiting SAM-dependent methyltransferases (MTases). Some MTases play significant roles in host-pathogen interactions, one of which is Clostridioides difficile-specific DNA adenine MTase (CamA). …
·pubmed.ncbi.nlm.nih.gov·
Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase - PubMed
Practical observations on the use of fluorescent reporter systems in Clostridioides difficile - PubMed
Practical observations on the use of fluorescent reporter systems in Clostridioides difficile - PubMed
Fluorescence microscopy is a valuable tool to study a broad variety of bacterial cell components and dynamics thereof. For Clostridioides difficile, the fluorescent proteins CFPsupopt/sup, mCherrysupOpt/sup and phiLOV2.1, and the self-labelling tags SNAPsupCd/sup and HaloTag, hereafter c …
·pubmed.ncbi.nlm.nih.gov·
Practical observations on the use of fluorescent reporter systems in Clostridioides difficile - PubMed
MTIG on Twitter
MTIG on Twitter
On April 24th, @PeggyFund will host the 2023 National C. diff Advocacy Summit. During the event #CDiff patients, caregivers and healthcare industry members will discuss their experiences and strategies to fight this potentially life-threatening disease. https://t.co/WMnMENqxvp— MTIG (@MTIG_News) April 19, 2023
·twitter.com·
MTIG on Twitter
Synthesis of Muramyl-δ-Lactam in Spore Peptidoglycan of Clostridioides difficile - PubMed
Synthesis of Muramyl-δ-Lactam in Spore Peptidoglycan of Clostridioides difficile - PubMed
Clostridioides difficile is a spore-forming human pathogen responsible for significant morbidity and mortality. Infections by this pathogen ensue dysbiosis of the intestinal tract, which lead to germination of the spores. The process of spore formation requires a transition for the cell-wall peptido …
·pubmed.ncbi.nlm.nih.gov·
Synthesis of Muramyl-δ-Lactam in Spore Peptidoglycan of Clostridioides difficile - PubMed
Synthesis of Muramyl-δ-Lactam in Spore Peptidoglycan of Clostridioides difficile - PubMed
Synthesis of Muramyl-δ-Lactam in Spore Peptidoglycan of Clostridioides difficile - PubMed
Clostridioides difficile is a spore-forming human pathogen responsible for significant morbidity and mortality. Infections by this pathogen ensue dysbiosis of the intestinal tract, which lead to germination of the spores. The process of spore formation requires a transition for the cell-wall peptido …
·pubmed.ncbi.nlm.nih.gov·
Synthesis of Muramyl-δ-Lactam in Spore Peptidoglycan of Clostridioides difficile - PubMed
Targeting the human gut microbiome with small-molecule inhibitors
Targeting the human gut microbiome with small-molecule inhibitors
Nature Reviews Chemistry - Small-molecule inhibitors offer many advantages for manipulating the gut microbiome, both as tool compounds and as potential therapeutics. This Review highlights recent...
·news.google.com·
Targeting the human gut microbiome with small-molecule inhibitors
Draft Genome Sequences and Genome Characterization of Three Toxigenic and Two Nontoxigenic Clostridioides difficile Clinical Isolates from Florida, USA - PubMed
Draft Genome Sequences and Genome Characterization of Three Toxigenic and Two Nontoxigenic Clostridioides difficile Clinical Isolates from Florida, USA - PubMed
Draft genome sequences of five Clostridioides difficile clinical isolates were obtained in Florida, USA. Three isolates, designated TGH29 (sequence type 1 [ST1]/clade 2), TGH79 (ST11/clade 5), and TGH91 (ST35/clade 1), contained toxin-encoding genes. The two nontoxigenic strains were classified as T …
·pubmed.ncbi.nlm.nih.gov·
Draft Genome Sequences and Genome Characterization of Three Toxigenic and Two Nontoxigenic Clostridioides difficile Clinical Isolates from Florida, USA - PubMed
CLO2309
CLO2309
Formstack Form - CLO2309
·banffcentreforartsandcreativity-qwlyf.formstack.com·
CLO2309
Clostridioides difficile minimal nutrient requirements for flagellar motility - PubMed
Clostridioides difficile minimal nutrient requirements for flagellar motility - PubMed
As many gastro-intestinal pathogens, the majority of Clostridioides difficile strains express flagella together with a complete chemotaxis system. The resulting swimming motility is likely contributing to the colonization success of this important pathogen. In contrast to the well investigate …
·pubmed.ncbi.nlm.nih.gov·
Clostridioides difficile minimal nutrient requirements for flagellar motility - PubMed
A leaky human colon model reveals uncoupled apical/basal cytotoxicity in early Clostridioides difficile toxin exposure | American Journal of Physiology-Gastrointestinal and Liver Physiology
A leaky human colon model reveals uncoupled apical/basal cytotoxicity in early Clostridioides difficile toxin exposure | American Journal of Physiology-Gastrointestinal and Liver Physiology
Clostridioides difficile (C. difficile) toxins A (TcdA) and B (TcdB) cause antibiotic-associated colitis in part by disrupting epithelial barrier function. Accurate in vitro models are necessary to detect early toxicity kinetics, investigate disease etiology, and develop preclinical models for new therapies. Properties of cancer cell lines and organoids inherently limit these efforts. We developed adult stem cell-derived monolayers of differentiated human colonic epithelium (hCE) with barrier function, investigated the impact of toxins on apical/basal aspects of monolayers, and evaluated whether a leaky epithelial barrier enhances toxicity. Single-cell RNA-sequencing (scRNAseq) mapped C. difficile-relevant genes to human lineages. Transcriptomics compared hCE to Caco-2, informed timing of in vitro stem cell differentiation, and revealed transcriptional responses to TcdA. Transepithelial electrical resistance (TEER) and fluorescent permeability assays measured cytotoxicity. Contribution of TcdB toxicity was evaluated in a diclofenac-induced leaky gut model. scRNAseq demonstrated broad and variable toxin receptor expression. Absorptive colonocytes in vivo displayed increased toxin receptor, Rho GTPase, and cell junction gene expression. Advanced TcdA toxicity generally decreased cytokine/chemokine and increased tight junction and death receptor genes. Differentiated Caco-2 cells remained immature whereas hCE monolayers were similar to mature colonocytes in vivo. Basal exposure of TcdA/B caused greater toxicity and apoptosis than apical exposure. Apical exposure to toxins was enhanced by diclofenac. Apical/basal toxicities are uncoupled with more rapid onset and increased magnitude postbasal toxin exposure. Leaky junctions enhance toxicity of apical TcdB exposure. hCE monolayers represent a physiologically relevant and sensitive system to evaluate the impact of microbial toxins on gut epithelium. NEW & NOTEWORTHY Novel human colonocyte monolayer cultures, benchmarked by transcriptomics for physiological relevance, detect early cytopathic impacts of Clostridioides difficile toxins TcdA and TcdB. A fluorescent ZO-1 reporter in primary human colonocytes is used to track epithelial barrier disruption in response to TcdA. Basal TcdA/B exposure generally caused more rapid onset and cytotoxicity than apical exposure. Transcriptomics demonstrate changes in tight junction, chemokine, and cytokine receptor gene expression post-TcdA exposure. Diclofenac-induced leaky epithelium enhanced apical exposure toxicity.
·journals.physiology.org·
A leaky human colon model reveals uncoupled apical/basal cytotoxicity in early Clostridioides difficile toxin exposure | American Journal of Physiology-Gastrointestinal and Liver Physiology
Gene network interaction analysis to elucidate the antimicrobial resistance mechanisms in the Clostridiumdifficile - PubMed
Gene network interaction analysis to elucidate the antimicrobial resistance mechanisms in the Clostridiumdifficile - PubMed
Antimicrobial resistance has caused chaos worldwide due to the depiction of multidrug-resistant (MDR) infective microorganisms. A thorough examination of antimicrobial resistance (AMR) genes and associated resistant mechanisms is vital to solving this problem. Clostridium difficile (C. difficile) is …
·pubmed.ncbi.nlm.nih.gov·
Gene network interaction analysis to elucidate the antimicrobial resistance mechanisms in the Clostridiumdifficile - PubMed
A Conserved Switch Controls Virulence, Sporulation, and Motility in C. difficile - PubMed
A Conserved Switch Controls Virulence, Sporulation, and Motility in C. difficile - PubMed
Spore formation is required for environmental survival and transmission of the human enteropathogenic Clostridioides difficile . In all bacterial spore formers, sporulation is regulated through activation of the master response regulator, Spo0A. However, the factors and mechanisms that direct …
·pubmed.ncbi.nlm.nih.gov·
A Conserved Switch Controls Virulence, Sporulation, and Motility in C. difficile - PubMed
GutMicrobiota Health on Twitter
GutMicrobiota Health on Twitter
Scientists succeeded in defining a new algorithm to systematically profile gut microbiome metabolism, identifying 19,890 gene clusters in 4,240 high-quality microbial genomes:https://t.co/c7GNu7mtiZ— GutMicrobiota Health (@GMFHx) April 7, 2023
·twitter.com·
GutMicrobiota Health on Twitter
Mechanism of germination inhibition of Clostridioides difficile spores by an aniline substituted cholate derivative (CaPA) - PubMed
Mechanism of germination inhibition of Clostridioides difficile spores by an aniline substituted cholate derivative (CaPA) - PubMed
Clostridioides difficile infection (CDI) is the major identifiable cause of antibiotic-associated diarrhea and has been declared an urgent threat by the CDC. C. difficile forms dormant and resistant spores that serve as infectious vehicles for CDI. To cause disease, C. difficile spores recognize tau …
·pubmed.ncbi.nlm.nih.gov·
Mechanism of germination inhibition of Clostridioides difficile spores by an aniline substituted cholate derivative (CaPA) - PubMed