Engineering probiotics to inhibit Clostridioides difficile infection by dynamic regulation of intestinal metabolism
Nature Communications - Clostridioides difficile infection (CDI) results in significant morbidity and mortality in hospitalised patients. Here the authors engineer probiotics to restore intestinal...
8 Must-Read GI Studies for the Primary Care Physician
Gastroenterologist Vivek Kaul, MD, reviews his top picks for primary care clinicians from the 2022 Digestive Disease Week, the premier meeting for the GI specialty.
C. difficile drives some colorectal cancers, study suggests: Common infection could be one driver of colorectal cancer rise in younger adults
Data suggest that Clostridioides difficile, or C. diff, a bacterial species well known for causing serious diarrheal infections, may also drive colorectal cancer.
Carriage of three plasmids in a single human clinical isolate of Clostridioides difficile | bioRxiv
A subset of clinical isolates of Clostridioides difficile contains one or more plasmids and these plasmids can harbor virulence and antimicrobial resistance determinants. Despite their potential importance, C. difficile plasmids remain poorly characterized. Here, we describe a human clinical isolate that carries three plasmids from three different plasmid families that are therefore compatible. For two of these, we identify a region capable of sustaining plasmid replication in C. difficile . Together, our data advance our understanding of C. difficile plasmid biology. ### Competing Interest Statement The authors have declared no competing interest.
Next we have Ashleigh Rogers (@AshleighPRogers) from @MonashUni who asks how does infection-associated damage from #Clostridoides difficile alters the colons ability to regenerate?They have found that #Cdiff infection resulted in sustained damage to the #GI tract.— Sarah Revitt-Mills, PhD (@Sci_Sez) July 13, 2022
Stool Toxin Concentration Does Not Distinguish Clostridioides difficile Infection from Colonization in Children Less Than 3 Years of Age | Journal of the Pediatric Infectious Diseases Society | Oxford Academic
Molecular epidemiology and antimicrobial resistance patterns of Clostridioides difficile isolates in Algerian hospitals
Overall prevalence of C. difficile in our healthcare settings was 6%. Antibiotic resistance rates ranged from 72.2% (clindamycin) to 16.6% (tetracycline). This study highlighted a relatively high genetic diversity in term of ribotypes, sequence types, toxin and antibiotic resistance patterns, in the …
Coordinated modulation of multiple processes through phase variation of a c-di-GMP phosphodiesterase in Clostridioides difficile - PubMed
The opportunistic nosocomial pathogen Clostridioides difficile exhibits phenotypic heterogeneity through phase variation, a stochastic, reversible process that modulates expression. In C. difficile, multiple sequences in the genome undergo inversion through site-specific recombination. Two such loci …
Gut associated metabolites and their roles in Clostridioides difficile pathogenesis - PubMed
The nosocomial pathogen Clostridioides difficile is a burden to the healthcare system. Gut microbiome disruption, most commonly by broad-spectrum antibiotic treatment, is well established to generate a state that is susceptible to CDI. A variety of metabolites produced by the host and/or gut …
Host and Clostridioides difficile-Response Modulated by Micronutrients and Glutamine: An Overview - PubMed
Changes in intestinal microbiota are integral to development of Clostridioides difficile (C. difficile)-associated nosocomial diarrhea. Certain diets, especially Western diets, increase susceptibility to C. difficile infection (CDI). Here, we discuss recent findings regarding ho …
Thiol Metabolism and Volatile Metabolome of Clostridioides difficile
Clostridioides difficile (previously Clostridium difficile) causes life-threatening gut infections. The central metabolism of the bacterium is strongly influencing toxin production and consequently the infection progress. In this context, the composition and potential origin of the vol …
An Unusual Presentation of Clostridioides Difficile Colitis in a Patient on Opioids
Clostridioides difficile colitis is an inflammation of the colon due to toxins produced by a gram-positive bacterium called Clostridioides difficile (also known as Clostridium difficile). Clostridioides difficile colitis is associated with an increased risk of morbidity a …
Clostridioides difficile infection in cancer patients receiving immune checkpoint inhibitors
CDI is common in ICI-treated cancer patients, especially those with IMDC requiring immunosuppressants. Antibiotics did not alter the need for immunosuppressants in those with concurrent IMDC. Use of PPI and antibiotics while receiving immunosuppressants for IMDC was associated with a greater risk of …
BIDMC Grand Rounds: J. Thomas Lamont, M.D. on Major Advances in Gastroenterology & Hepatology - C. Diff In 30 Minutes: The Book
In January 2020, J. Thomas Lamont, M.D. gave a grand rounds presentation. He was looking back at Major Advances in Gastroenterology & Hepatology from 1965 to the present.
Absence of toxin gene transfer from Clostridioides difficile strain 630Δerm to nontoxigenic C. difficile strain NTCD-M3r in filter mating experiments - PubMed
Nontoxigenic Clostridioides difficile strain M3 (NTCD-M3) protects hamsters and humans against C. difficile infection. Transfer in vitro of the pathogenicity locus (PaLoc) to nontoxigenic strain CD37 has been reported. We repeated these conjugations using toxigenic strain 630Δerm as donor and NTCD-M …
Strain Variation in Clostridioides difficile Cytotoxicity Associated with Genomic Variation at Both Pathogenic and Nonpathogenic Loci - PubMed
Clinical disease from Clostridioides difficile infection can be mediated by two toxins and their neighboring regulatory genes located within the five-gene pathogenicity locus (PaLoc). We provide several lines of evidence that the cytotoxicity of C. difficile may be modulated by genomic variants outs …
An Artificial Microbiome Consortium Prevents Recurrence of Clostridioides difficile Infection: Paving the Way for Fecal Microbiota Transplantation 2.0 - Gastroenterology
Molecular characterization and antibiotic resistance of Clostridioides difficile in patients with inflammatory bowel disease from two hospitals in China
The incidence and molecular epidemiology of C. difficile infection in adult IBD patients resembled CDI in the general inpatient population. A higher antibiotic resistance rate was identified among the C. difficile isolates obtained from pediatric IBD patients than adult patients, and few STs account …
Molecular characterization and antibiotic resistance of Clostridioides difficile in patients with inflammatory bowel disease from two hospitals in China
The incidence and molecular epidemiology of C. difficile infection in adult IBD patients resembled CDI in the general inpatient population. A higher antibiotic resistance rate was identified among the C. difficile isolates obtained from pediatric IBD patients than adult patients, and few STs account …
Imaging Clostridioides difficile Spore Germination and Germination Proteins
Clostridioides difficile spores are the infective form for this endospore-forming organism. The vegetative cells are intolerant to oxygen and poor competitors with a healthy gut microbiota. Therefore, in order for C. difficile to establish infection, the spores have to germinate in an environment th …
Clinical Determinants Predicting Clostridioides difficile Infection among Patients with Chronic Kidney Disease - PubMed
The majority of recently published studies indicate a greater incidence rate and mortality due to Clostridioides difficile infection (CDI) in patients with chronic kidney disease (CKD). The aim of this study was to assess the clinical determinants predicting CDI among hospitalized patients with CKD …
Histologic Features of Clostridioides difficile Colitis in Patients with Inflammatory Bowel Disease - PubMed
Although colonic biopsies from IBD patients with C. difficile infection usually lack features that aid distinction from colitic flares, some cases show an acute colitis pattern not seen in IBD alone. When identified in biopsies from symptomatic IBD patients, these changes should alert pathologists t …
