C Diff Molecular

Food microbiologist Dennis D’Amico demonstrated that bacteriophages can effectively reduce the amount of common foodborne pathogens in milk

Request PDF | Clostridioides (Clostridium) difficile infection in hospitalized adult patients in Cambodia | Despite high levels of global concern, little is known about the epidemiology of Clostridioides ( Clostridium ) difficile infection (CDI) in... | Find, read and cite all the research you need on ResearchGate

The major risk factors associated with CDI in IBD patients were IBD activity before infection, UC and colonic Crohn's disease, short duration of IBD, corticosteroid usage, and hospitalization. Patients with active IBD and a shorter disease duration may benefit from more frequent follow-ups in the ea …

Clostridioides difficile infections are the leading cause of healthcare-associated diarrhea. C. difficile poses a risk to public health due to its ability to form spores and cause recurrent infections. Glycolipids make up ~50% of the polar lipids in the C. difficile membrane, a …

The rapid detection and containment of healthcare-associated infections (HCAIs) is critical in preventing and controlling infectious disease outbreaks within healthcare settings. Whole genome sequencing (WGS) has emerged as a powerful tool for tracking the transmission dynamics of pathogens and when …

Clostridiodes difficile is a gram-positive, spore-forming obligate anaerobe bacillus found in the intestines of healthy individuals without signs of disease. It may cause diarrhea after antibiotic use due to the eradication of the normal gut flora. Most cases resolve with proper treatment, bu …

Composed of an elaborate ecosystem of bacteria, fungi, viruses and protozoa residing in the human digestive tract, the gut microbiome influences metabolism, immune modulation, bile acid homeostasis and host defence. Through observational and preclinical data, the gut microbiome has been implicated i …

spaniClostridioides difficile/i (iC. difficile/i) infections (CDIs) are typically intestinal but can also present extraintestinally, a rare occurrence with limited literature. This case describes a woman with cirrhosis who developed spontaneous bacterial peritonitis (SBP) caused by monomicrobial/span …

A CDI prior to TKA is an independent risk factor for PJI. Closer proximity of CDI to surgery is associated with a "dose-dependent" increased PJI risk. Surgeons should consider delaying TKA until a minimum of one year after a diagnosis of CDI.

IBD significantly increases the risk of CDI among patients treated with biologic therapy; although such treatments do not seem to individually increase the risk, the number of biologics received may be a new predictor of CDI.

Bacterial spores formed upon metabolic stress have minimal metabolic activity and can remain dormant for years. Nevertheless, they can sense the environment and germinate quickly upon exposure to various germinants. Germinated spores can then outgrow into vegetative cells. Germination of spores of s …

The monitoring of gut colonization by MDR microorganisms in a single LTCF for 1 year demonstrated the predominance of ESBL-producing iE. coli/i. Almost half of the residents were resistant to its colonization, whereas in 15.5% of them gut colonization was stable. Incidence of infectious episodes …

Our work provides, for the first time, genomic insights into C. difficile strains present in Mexico. In our hospital setting, the predominant strains were primarily NAP1/RT027 and exhibited resistance to linezolid, a pattern observed in both pediatric and adult populations. This unique combination o …

Dietary intervention with the exclusion of potential allergens from poultry meat and egg sources can be an effective approach for the management of canine atopic dermatitis.

Clostridioides difficile infection (CDI) is an urgent public health threat with a high rate of recurrence and limited treatment options. In vivo models have been indispensable in understanding CDI pathophysiology and establishing treatment protocols and continue to be essential in pre- …

This study delves into a newly discovered MBL (metallo-β-lactamase) in Clostridioides difficile, a formidable pathogen known for causing nosocomial infections and exhibiting resistance to antimicrobial agents. The primary objective was to unravel its structure-function relationship. This rese …

Background Clostridioides difficile infection (CDI) in inflammatory bowel disease (IBD) has been associated with poor clinical outcomes. The relationship between biologic therapy and CDI is controversial. We aimed to assess whether biologic therapy increases CDI risk among IBD patients, to identify factors associated with increased CDI risk, and to characterize CDI episodes in our population. Methods We included patients diagnosed with IBD (IBD-cohort) and immune-mediated inflammatory rheumatic diseases (Rheuma-cohort). Risk factors for CDI were assessed using a logistic regression model. We also estimated the incidence rate of CDI for each biologic. Results We included 1866 patients: 1041 from the IBD-cohort and 825 from the Rheuma-cohort. The diagnosis of IBD was the major risk factor for developing CDI in the overall population (OR: 18.29, CI 95%: 5.59–59.80, p  0.001). Within the IBD-cohort, patients with ulcerative colitis had an increased risk for CDI compared to Crohn’s disease (OR:2.00, 95% CI: 1.18–3.42, p = 0.011). Although the subgroup of IBD patients receiving biologics showed a higher incidence of CDI compared to unexposed IBD patients, biologic therapy was not an independent risk factor for CDI in the logistic regression model; nevertheless, patients who received 3 or more biologic agents had a significantly higher risk for CDI (OR: 3.09, CI 95% 1.13–8.47, p = 0.028). Conclusions IBD significantly increases the risk of CDI among patients treated with biologic therapy; although such treatments do not seem to individually increase the risk, the number of biologics received may be a new predictor of CDI.

This study highlights iC. difficile/i's adaptability and genetic diversity. The decline in toxin genes reflects fewer toxigenic isolates, but the bacterium's increasing preserved resistance factors and virulence genes enable its rapid evolution. ST2, ST42, and ST8 dominate globally, emphasizing …

spaniClostridioides difficile/i is a leading cause of nosocomial antibiotic-associated diarrhea in developed countries with many known virulence factors. In several pathogens, motility and virulence are intimately linked by regulatory networks that allow coordination of these processes in pathogenes/span …

Spore germination in iClostridioides difficile/i is initiated by a cascade of activities of several proteins that culminates in the activation of SleC, a cell-wall-processing enzyme. We report herein the details of the enzymatic activities of SleC by the use of synthetic peptidoglycan fragments …

Cyclospora cayetanensis, a parasite causing human enteritis, has been rarely reported from healthcare laboratories despite its universal presence. While it is usually responsible for self-limiting diarrhea in immunocompetent individuals, we present a case where the patient suffered from severe, unre …

The investigated gene locus codes for an oxidative stress operon whose members have the potential to neutralize Osub2/subsup●-/sup and Hsub2/subOsub2/sub to water and thus complements the arsenal of ROS detoxifying mechanisms that are already known in C. difficile. However, full acti …

Inclusion of hypotension, defined as SBP ≤90 mm Hg or MAP ≤65 mm Hg, was an important component of FCDI severity criteria, significantly improving the predictive ability to identify FCDI patients at risk for poor outcomes.

Food allergy is a complex disease, with multiple environmental factors involved. Considering the regulatory effect of Clostridium difficile toxin A (Tcd A) on biological processes of allergic reactions, the role of oral exposure to Tcd A on food allergy was investigated. The intestinal permea …

Splenic abscesses are rare but serious infections often linked to immunosuppressive conditions. While iClostridioides difficile/i is well-known for causing colitis, its occurrence in locations outside the gastrointestinal tract, like the spleen, is exceedingly rare. This report highlights a uniq …

The investigated gene locus codes for an oxidative stress operon whose members have the potential to neutralize O2●- and H2O2 to water and thus complements the arsenal of ROS detoxifying mechanisms that are already known in C. difficile. However, full acti …

A metagenome-informed metaproteomics approach unravels the interplay between the host, gut microbiome, and diet in animal models as well as in human cohorts and identifies disease biomarkers of dysbiosis and gut disorders.

Spore germination in Clostridioides difficile is initiated by a cascade of activities of several proteins that culminates in the activation of SleC, a cell-wall-processing enzyme. We report herein the details of the enzymatic activities of SleC by the use of synthetic peptidoglycan fragments …

Clostridioides difficile is a leading cause of nosocomial antibiotic-associated diarrhea in developed countries with many known virulence factors. In several pathogens, motility and virulence are intimately linked by regulatory networks that allow coordination of these processes in pathogenes …

Genomic data on iClostridioides difficile/i from the African continent are currently lacking, resulting in the region being under-represented in global analyses of iC. difficile/i infection (CDI) epidemiology. For the first time in Nigeria, we utilized whole-genome sequencing and phylogeneti …