C Diff Molecular

The alternative σ factor TcdR controls the synthesis of two major enterotoxins: TcdA and TcdB in Clostridioides difficile. Four potential TcdR-dependent promoters in the pathogenicity locus of C. difficile showed different activities. In this study, we constructed a heterologous system …

Researchers uncover characteristics of ARE-ABCF proteins in three bacterial species, shedding light on their function and potent combined resistance.

A new study published in the peer-reviewed OMICS: A Journal of Integrative Biology identified genes for virulence and antimicrobial resistance in two

A new study published in OMICS: A Journal of Integrative Biology has identified genes for virulence and antimicrobial resistance in two bacteria that co-occur with C. difficile, suggesting these pathogens ...

Much of our current knowledge about cellular RNA-protein complexes in bacteria is derived from analyses in gram-negative model organisms, with the discovery of RNA-binding proteins (RBPs) generally lagging behind in Gram-positive species. Here, we have applied Grad-seq analysis of native RNA-protein …

Clostridioides difficile infection associated to gut microbiome dysbiosis is the leading cause for nosocomial diarrhea. The ability of C. difficile to form biofilms has been progressively linked to its pathogenesis as well as its persistence in the gut. Although C. difficile has …

Based on these findings, we conclude that clpC plays a role in the virulence properties of C. difficile.

Clostridioides difficile (CD) is a major planetary health burden. A Gram-positive opportunistic pathogen, CD, colonizes the large intestine and is implicated in sepsis, pseudomembranous colitis, and colorectal cancer. C. difficile infection typically following antibiotic exposure resul …

logic mutations leading to clonal hematopoiesis (CH) may further increase vulnerability to adverse sequelae. This review summarizes the available literature examining genetic predispositions to severe COVID-19 and describes how these findings could eventually be used to improve its clinical management. DATA SOURCES: A PubMed literature search was performed. STUDY SELECTION: Studies examining the significance of inherited genetic variation or acquired CH mutations in severe COVID-19 were selected for inclusion. DATA EXTRACTION: Relevant genetic association data and aspects of study design were qualitatively assessed and narratively synthesized. DATA SYNTHESIS: Genetic variants affecting inflammatory responses may increase susceptibility to severe COVID-19. Genome-wide association studies and candidate gene approaches have identified a list of inherited mutations, which likely alter cytokine and interferon secretion, and lung-specific mechanisms of immunity in COVID-19. The potential role of CH in COVID-19 is more uncertain at present; however, the available evidence suggests that the various types of acquired mutations and their differential influence on immune cell function must be carefully considered. CONCLUSIONS: The current literature supports the hypothesis that host genetic factors affect vulnerability to severe COVID-19. Further research is required to confirm the full scope of relevant variants and the causal mechanisms underlying these associations. Clinical approaches, which consider the genetic basis of interindividual variability in COVID-19 and potentially other causes of critical illness, could optimize hospital resource allocation, predict responsiveness to treatment, identify more efficacious drug targets, and ultimately improve outcomes....

Sow colostrum has been reported to protect the IPEC-J2 cells and piglet colon tissues from detrimental effect of Clostridioides difficile toxins. Since dietary fibre can influence the colostrum composition in sows, we hypothesised that it can also differentially affect the colostrum potential agains …

#Cdiff is known to colonize in the large intestine of humans. #guthealth #GITwitter #IDTwitter pic.twitter.com/cmi7ySohdi— Microbiome Science (@MbiomeScience) May 17, 2023

Biology student Paige Hopkinson has been awarded the new One Health Summer Research Studentship, a partnership between the Global Nexus School for Pandemic Prevention and Response at McMaster and the University of Guelph's One Health Institute.

A species of ordinary gut bacteria that we all carry flourishes when the intestinal flora is knocked out by a course of antibiotics. Since the bacteria is naturally resistant to many antibiotics, it causes problems, particularly in healthcare settings. A study led by Lund University in Sweden now sh

Nature Reviews Microbiology - In this Review, Carmody and Bisanz examine the role of the gut microbiome in energy metabolism, the two-way relationship between the gut microbiome and weight...

Clostridioides difficile (CD) is a major planetary health burden. A Gram-positive opportunistic pathogen, CD, colonizes the large intestine and is implicated in sepsis, pseudomembranous colitis, and colorectal cancer. C. difficile infection typically following antibiotic exposure resul …

Using a diverse human gut community, researchers investigated how gut microbiota interspecies interactions affected the response of Clostridioides difficile to vancomycin and metronidazole.

This study examines how interactions between constituent members of the human gut microbiome influence the pathogen C. difficile’s response to clinically relevant antibiotics, providing key insights into ecological principles and molecular mechanisms that influence antibiotic susceptibility in this health-relevant system.

Clostridioides difficile (C. difficile) is responsible for a high percentage of gastrointestinal infections and its pathological activity is due to toxins A and B. C. difficile infection (CDI) is increasing worldwide due to the unstoppable spread of C. difficile in the an …

Researchers suggest that entire gut microbiome should be considered when designing antibiotic treatments, after finding that the susceptibility of the pathogen Clostridioides difficile depends on the presence of other bacteria.

Despite antecedent risk factors for CDI, the overall prevalence of CDI in ASUC was low and the outcomes were determined by underlying disease severity.

Clostridioides difficile infection (CDI) represents a significant challenge to public health. C. difficile-associated mortality and morbidity have led the U.S. CDC to designate it as an urgent threat. Moreover, recurrence or relapses can occur in up to a third of CDI patients, due in part to antibio …

Clostridioides difficile colonizes up to 30-40% of community-dwelling adults without causing disease 1, 2 . C. difficile infections (CDIs) are the leading cause of antibiotic-associated diarrhea in the U.S. 3, 4 and typically develop in individuals following disru …

The ability of bacterial pathogens to establish recurrent and persistent infections is frequently associated with their ability to form biofilms. Clostridioides difficile infections have a high rate of recurrence and relapses and it is hypothesized that biofilms are involved in its pathogenicity and …

In the human gut, the growth of the pathogen Clostridioides difficile is impacted by a complex web of interspecies interactions with members of human gut microbiota. We investigate the contribution of interspecies interactions on the antibiotic response of C. difficile to clinically relevant antibio …

Research provides answers to the long-standing mystery of bacterial spores, illuminating new paths for disease prevention. Inert, sleeping bacteria — or spores — can survive for years, even centuries, without nutrients, resisting heat, UV radiation, antibiotics, and other harsh chemicals. Ho

Higher BMI and exposure to antibiotics are risk factors for C. difficile asymptomatic bacterial colonization in kids with cystic fibrosis.

A new study showed an introduction of beneficial microbiota.

Clostridioides difficile colonizes up to 30-40% of community-dwelling adults without causing disease 1, 2 . C. difficile infections (CDIs) are the leading cause of antibiotic-associated diarrhea in the U.S. 3, 4 and typically develop in individuals following disru …

Several studies suggest that butyrate may be important in alleviating gut infections, such as reducing inflammation caused by the healthcare-associated Clostridioides difficile . While studies in both animal models and human studies correlate high levels of butyrate with reduced C. diffici …

A species of ordinary gut bacteria that we all carry flourishes when the intestinal flora is knocked out by a course of antibiotics.