#Microbiome therapeutics have the potential to revolutionize the treatment of several diseases and medical conditions including #CDiff, IBD, #AlzheimersDisease, #cancer, #diabetes. Learn more here: https://t.co/UaYhHR2WMp— MTIG (@MTIG_News) October 17, 2022
Paper alert! - our work on #plasmid-mediated metronidazole resistance in #cdiff continues. https://t.co/faaJPRbWTl A small 🧵thread to highlight key points 👇 1/6— Wiep Klaas Smits (@SmitsLab) October 17, 2022
Current and future applications of fecal microbiota transplantation for children
Fecal microbiota transplantation (FMT) is a new and adequate route to modify the microbial ecosystem in gastrointestinal tract of the hosts. Intestina…
Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile - PubMed
Some bacteria express a binary toxin translocation system, consisting of an enzymatic subunit and translocation pore, that delivers enzymes into host cells through endocytosis. The most clinically important bacterium with such a system is Clostridioides difficile (formerly Clostridium). The CDTa and …
Bile acids and the gut microbiota: metabolic interactions and impacts on disease
Nature Reviews Microbiology - The gut microbiota metabolizes bile acids, thereby influencing human health and diseases including obesity, colitis and cancer. In this Review, Patterson and...
Assessing the clinical value of faecal bile acid profiling to predict recurrence in primary Clostridioides difficile infection - PubMed
Gut BA metabolism dynamics differ in primary CDI patients between those developing recurrence and those who do not. Individual BAs show promise as potential novel biomarkers to predict CDI recurrence.
In-depth characterization of the Clostridioides difficile phosphoproteome to identify Ser/Thr kinase substrates - PubMed
Clostridioides difficile is the leading cause of post-antibiotic diarrhrea in adults. During infection, the bacterium must rapidly adapt to the host environment by using survival strategies. Protein phosphorylation is a reversible post-translational modification employed ubiquitously for signal tran …
Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile - Nature.com
Nature Communications - Binary toxin translocation system consists of an enzymatic subunit and translocation pore. Here, the authors report the high-resolution cryo-EM structure of Clostridioides...
Monique BrouilletteA flurry of microbiome research over the past two decades has led to many insights about the link between the microbes and health, but now the race to the clinic is on.
Advancing human gut microbiota research by considering gut transit time | Gut
Accumulating evidence indicates that gut transit time is a key factor in shaping the gut microbiota composition and activity, which are linked to human health. Both population-wide and small-scale studies have identified transit time as a top covariate contributing to the large interindividual variation in the faecal microbiota composition. Despite this, transit time is still rarely being considered in the field of the human gut microbiome. Here, we review the latest research describing how and why whole gut and segmental transit times vary substantially between and within individuals, and how variations in gut transit time impact the gut microbiota composition, diversity and metabolism. Furthermore, we discuss the mechanisms by which the gut microbiota may causally affect gut motility. We argue that by taking into account the interindividual and intraindividual differences in gut transit time, we can advance our understanding of diet–microbiota interactions and disease-related microbiome signatures, since these may often be confounded by transient or persistent alterations in transit time. Altogether, a better understanding of the complex, bidirectional interactions between the gut microbiota and transit time is required to better understand gut microbiome variations in health and disease.
Iqbal: Severe C. difficile infection is defined by high white blood cell count, increased serum creatinine, pyrexia and radiological evidence of severe colitis and leaky gut @esnm_eu #GMFHCoverage pic.twitter.com/5cVc1Duih9— GutMicrobiota Health (@GMFHx) October 12, 2022
C. difficile Patient Journey - Gastrointestinal Society
C. difficile Patient Journey C. difficile infection (CDI) occurs when Clostridioides difficile bacteria grow out of control in the digestive tract. The bacteria and their spores are resistant to many common cleaning methods so they can transfer easily between surfaces if people do not wash their hands adequately
FENS Friday webinar “The microbiome, immunity, and neurodevelopment trialogue: from womb to adulthood” - Federation of European Neuroscience Societies
Registration for the FENS Friday webinar on “The microbiome, immunity, and neurodevelopment trialogue: from womb to adulthood” is now open. The microbiome topic has emerged as a field of great…
Multi-omic analyses identify mucosa bacteria and fecal metabolites associated with weight loss after fecal microbiota transplantation
Fecal microbiota transplantation (FMT) has shown promising results in animal models of obesity, while results in human studies are inconsistent. We ai…
Clostridioides difficile and neurological disorders: New perspectives - PubMed
Despite brain physiological functions or pathological dysfunctions relying on the activity of neuronal/non-neuronal populations, over the last decades a plethora of evidence unraveled the essential contribution of the microbial populations living and residing within the gut, called gut microbiota. T …
From signal transduction to protein toxins-a narrative review about milestones on the research route of C. difficile toxins - PubMed
Selected findings about Clostridioides difficile (formerly Clostridium difficile) toxins are presented in a narrative review. Starting with a personal view on research about G proteins, adenylyl cyclase, and ADP-ribosylating toxins in the laboratory of Günter Schultz in Heidelberg, milestones of C. …
Reference Susceptibility Testing and Genomic Surveillance of Clostridioides difficile, United States, 2012-17
Elevated MICs to antibiotics used for treatment of C. difficile infection were rare and there was no increase in MICs over time. The lack of vanA/B genes or mutations consistently associated with elevated vancomycin MICs suggests there are multifactorial mechanisms of resistance. Ongoing surveillanc …
Clostridioides difficile and neurological disorders: New perspectives
Despite brain physiological functions or pathological dysfunctions relying on the activity of neuronal/non-neuronal populations, over the last decades a plethora of evidence unraveled the essential contribution of the microbial populations living and residing within the gut, called gut microbiota. T …
From signal transduction to protein toxins-a narrative review about milestones on the research route of C. difficile toxins
Selected findings about Clostridioides difficile (formerly Clostridium difficile) toxins are presented in a narrative review. Starting with a personal view on research about G proteins, adenylyl cyclase, and ADP-ribosylating toxins in the laboratory of Günter Schultz in Heidelberg, milestones of C. …
Microbiome epidemiology and association studies in human health | Nature Reviews Genetics
Nature Reviews Genetics - Microbiome epidemiology associates microbial community features with health outcomes, traits or exposures in human host populations. In this Review, the authors discuss...
On episode 002 of the Inside Matters Podcast, I speak with Dr Ben Mullish about DIY #FMT and how the field has evolved. We also discuss some super interesting new research that links the #gut #microbiome to #cancer. Tune in tomorrow to hear more: https://
On episode 002 of the Inside Matters Podcast, I speak with Dr Ben Mullish about DIY #FMT and how the field has evolved. We also discuss some super interesting new research that links the #gut #microbiome to #cancer. Tune in tomorrow to hear more: https://t.co/MOognlRkYS pic.twitter.com/2OaKgSf8jb— drjamesmcilroy (@drjamesmcilroy) October 5, 2022
The human body plays host to hundreds of diverse species of microbial life that have an enormous impact on human health and longevity. These microscopic organisms make up the human microbiome, and each person’s community of microbes is unique. Healthy bodies have a beneficial balance of “good” and “bad” bacteria, called symbiosis. But ...
Characterization of the glmS ribozymes from Listeria monocytogenes and Clostridium difficile
The glmS ribozyme regulates the expression of the essential GlmS enzyme being involved in cell wall biosynthesis. While 450 variants of the glmS ribozyme were identified by in silico approaches and homology searches, only a few have yet been experimentally investigated. Herein, we validate and …
Analysis of biofilm production and expression of adhesion structures of circulating Clostridioides difficile strains from Mexico
Proteins seem to have an important role in the biofilm's initial adherence and maturation. slpA and cwp84 are differentially expressed by C. difficile ribotype and biofilm production level.
