The clostridial diseases of horses can be divided into three major groups: enteric/enterotoxic, histotoxic, and neurotoxic. The main enteric/enterotoxic diseases include those produced by Clostridium perfringens type C and Clostridioides difficile, both of which are characterized by en …
Structure and assembly of the S-layer in C. difficile
Many bacteria and archaea possess a two-dimensional protein array, or S-layer, that covers the cell surface and plays crucial roles in cell physiology. Here, we report the crystal structure of SlpA, the main S-layer protein of the bacterial pathogen Clostridioides difficile, and use electron microsc …
Toxin gene profiles and antimicrobial resistance of Clostridioides difficile infection: a single tertiary care center study in Iran
Relative high resistance was detected towards metronidazole and vancomycin, although, still have acceptable activity for CDI treatment. However, a proper plan for the use of antibiotics and more regular screening of C. difficile antibiotic resistance seems necessary.
7. The #Cdiff #BugSlayer is very tightly packed, with pores of only about 11A, so only small ions can diffuse through #SlayerStructure 9/n
7. The #Cdiff #BugSlayer is very tightly packed, with pores of only about 11A, so only small ions can diffuse through #SlayerStructure 9/n pic.twitter.com/3dThsJw109— Bug Slayers (@BugS_layers) February 25, 2022
Structure and assembly of the S-layer in C. difficile | Nature Communications
Nature Communications - The S-layer is a two-dimensional protein array that covers the cell surface of many bacteria and archaea. Here, the authors use high-resolution X-ray crystallography and...
Antibiotic-resistant superbug has a protein coat like chain mail - New Scientist
The outer coat of the antibiotic-resistant bacterium Clostridium difficile has a structure similar to chain mail that stops drugs and immune system cells from killing it
Spectacular structure of C.difficile's protective armor revealed - News-Medical.net
The spectacular structure of the protective armor of superbug C.difficile has been revealed for the first time showing the close-knit yet flexible outer layer – like chain mail.
Flagellum and toxin phase variation impacts intestinal colonization and disease development in a mouse model of Clostridioides difficile infection
Clostridioides difficile is a major nosocomial pathogen that can cause severe, toxin-mediated diarrhea and pseudomembranous colitis. Recent work has shown that C. difficile exhibits heterogeneity in swimming motility and toxin production in vitro through phase variation by site- …
“#Cdiff is considered to be a #pathogen, but our data suggests that it is a common #commensal in healthy #infants. How we label cdiff matters, because it can affect our perception of the species 🦠 and how we decide to investigate it 🔎“
Assembly of the exosporium layer in Clostridioides difficile spores
Clostridioides difficile is a Gram-positive, spore-forming obligate anaerobe and a major threat to the healthcare system world-wide. Because of its strict anaerobic requirements, the infectious and transmissible morphotype is the dormant spore. During infection, C. difficile produces spores that can …
Glucosyltransferase-dependent and independent effects of Clostridioides difficile toxins during infection
Clostridioides difficile infection (CDI) is the leading cause of nosocomial diarrhea and pseudomembranous colitis in the USA. In addition to these symptoms, patients with CDI can develop severe inflammation and tissue damage, resulting in life-threatening toxic megacolon. CDI is mediated by two larg …
@AJMC_Journal and #Cdiff experts recently discussed the impact #rCDI can have on a #patient. Click here to watch their discussion: https://t.co/1zABGYbYGK pic.twitter.com/Nq6xYqmxXp— Rebiotix Inc. (@Rebiotix) February 15, 2022
Identification of novel, cryptic Clostridioides species isolates from environmental samples collected from diverse geographical locations
Clostridioides difficile is a pathogen often associated with hospital-acquired infection or antimicrobial-induced disease; however, increasing evidence indicates infections can result from community or environmental sources. Most genomic sequencing of C. difficile has focused on clinic …
Crosstalk Between the Gut Microbiota and Epithelial Cells Under Physiological and Infectious Conditions
The gastrointestinal tract (GIT) is considered the largest immunological organ, with a diverse gut microbiota, that contributes to combatting pathogens and maintaining human health. Under physiological conditions, the crosstalk between gut microbiota and intestinal epithelial cells (IECs) plays a cr …
Multiple Regulatory Mechanisms Control the Production of CmrRST, an Atypical Signal Transduction System in Clostridioides difficile
Clostridioides difficile, an intestinal pathogen and leading cause of nosocomial infection, exhibits extensive phenotypic heterogeneity through phase variation. The signal transduction system CmrRST, which encodes two response regulators (CmrR and CmrT) and a sensor kinase (CmrS), impacts C. diffici …
Your microbiome shapes your life. But where did it come from?
The gut microbiome is an ecosystem of hundreds to thousands of microbial species living within the human body. These populations affect our health, fertility, and even our longevity. But how do they
Binary Toxin Expression by Clostridioides difficile Is Associated With Worse Disease
Patients with fecal samples that were positive for CDT had increased disease severity and worse clinical outcomes. Utilization of PCR and testing for C. difficile toxins A and B may not reveal the entire picture when diagnosing CDI; detection of CDT-expressing strains is valuable in identifyi …
FinnGen, Metabolon to Incorporate Metabolomics in Study of Finnish Population - GenomeWeb
The partners hope that metabolic profiles of carriers of disease-associated genetic variants may offer insights into the basic biology of disease processes.
The first time I had a Clostridioides difficile infection, I was 37 years old. After a sequential antibiotic cocktail of amoxicillin, penicillin, metronidazole, and clindamycin prescribed to combat an infection in my jaw, I had the first wave of symptoms. Even having a long history of stomach-related issues dating back to my childhood, these new symptoms were like nothing I had ever experienced. Anything I consumed quickly traversed my gastrointestinal system within an impressively short timeframe, leaving me with a feeling of perpetual emptiness and exhaustion, a heightened awareness of the nearest restroom facilities, and an existential appreciation for saltine crackers.
VIDEO: Microbiome Health Index predicts post-antibiotic dysbiosis
The Microbiome Health Index for post-Antibiotic dysbiosis was a promising biomarker for predicting antibiotic dysbiosis and subsequent microbiome restoration, according to research published in Frontiers of Microbiology. “It’s very important that we maintain a healthy microbiome, but there are things that can disrupt or perturb a healthy microbiome to a less healthy state, which we
Bacterial and Fungal Gut Dysbiosis and Clostridium difficile in COVID-19: A Review
In COVID-19 there is bacterial and fungal dysbiosis that correlates with systemic and pulmonary inflammation, and illness severity. Further investigations are warranted to determine the efficacy of bacteriotherapy and FMT for modulating gut dysbiosis in COVID-19.
Surface-displayed glycopolymers of Clostridioides difficile
Clostridioides difficile is a leading cause of the healthcare-associated disease C. difficile infection (CDI), which has an annual US burden of over 200 000 cases. CDI mitigation strategies have been complicated by the emergence, and widespread distribution, of phylogenetically diverse lineages, as …
Outcomes, Disease Severity in Patients With Clostridioides difficile Infection With Binary Toxin-Expressing Strains - Infectious Disease Advisor
Investigators conducted a study that assessed disease severity and outcomes in patients with Clostridioides difficile infection with C difficile binary toxin-expressing strains.
Cellular adaptation of Clostridioides difficile to high salinity encompasses a compatible solute-responsive change in cell morphology
Infections by the pathogenic gut bacterium Clostridioides difficile cause severe diarrheas up to a toxic megacolon and are currently among the major causes of lethal bacterial infections. Successful bacterial propagation in the gut is strongly associated with the adaptation to changing nutrition-cau …
