An Artificial Microbiome Consortium Prevents Recurrence of Clostridioides difficile Infection: Paving the Way for Fecal Microbiota Transplantation 2.0 - Gastroenterology
C Diff Molecular
Molecular characterization and antibiotic resistance of Clostridioides difficile in patients with inflammatory bowel disease from two hospitals in China
The incidence and molecular epidemiology of C. difficile infection in adult IBD patients resembled CDI in the general inpatient population. A higher antibiotic resistance rate was identified among the C. difficile isolates obtained from pediatric IBD patients than adult patients, and few STs account …
Molecular characterization and antibiotic resistance of Clostridioides difficile in patients with inflammatory bowel disease from two hospitals in China
The incidence and molecular epidemiology of C. difficile infection in adult IBD patients resembled CDI in the general inpatient population. A higher antibiotic resistance rate was identified among the C. difficile isolates obtained from pediatric IBD patients than adult patients, and few STs account …
Imaging Clostridioides difficile Spore Germination and Germination Proteins
Clostridioides difficile spores are the infective form for this endospore-forming organism. The vegetative cells are intolerant to oxygen and poor competitors with a healthy gut microbiota. Therefore, in order for C. difficile to establish infection, the spores have to germinate in an environment th …
Clinical Determinants Predicting Clostridioides difficile Infection among Patients with Chronic Kidney Disease - PubMed
The majority of recently published studies indicate a greater incidence rate and mortality due to Clostridioides difficile infection (CDI) in patients with chronic kidney disease (CKD). The aim of this study was to assess the clinical determinants predicting CDI among hospitalized patients with CKD …
Histologic Features of Clostridioides difficile Colitis in Patients with Inflammatory Bowel Disease - PubMed
Although colonic biopsies from IBD patients with C. difficile infection usually lack features that aid distinction from colitic flares, some cases show an acute colitis pattern not seen in IBD alone. When identified in biopsies from symptomatic IBD patients, these changes should alert pathologists t …
Gut Microbiota and Metabolite Changes in Patients With Ulcerative Colitis and Clostridioides difficile Infection - PubMed
Our study identified 11 differential genera and 16 perturbed metabolites in patients with UC and CDI compared to those with UC alone. These findings may guide the design of research on potential mechanisms and specific treatments for CDI in patients with UC.
Age-associated gut microbiota impairs hippocampus-dependent memory in a vagus-dependent manner - PubMed
Aging is known to be associated with hippocampus-dependent memory decline, but the underlying causes of this age-related memory impairment remain yet highly debated. Here we showed that fecal microbiota transplantation (FMT) from aged, but not young, animal donors in young mice is sufficient to trig …
Histologic Features of Clostridioides difficile Colitis in Patients with Inflammatory Bowel Disease - PubMed
Although colonic biopsies from IBD patients with C. difficile infection usually lack features that aid distinction from colitic flares, some cases show an acute colitis pattern not seen in IBD alone. When identified in biopsies from symptomatic IBD patients, these changes should alert pathologists t …
[Clostridioides difficile toxin B gen amplification cycle threshold as a predictor of severity] - PubMed
Basándonos en los resultados de nuestro estudio, no ha sido posible la implementación sistemática del valor Ct como predictor de gravedad al informe clínico, no siendo posible extrapolar esta variable predictora de enfermedad por C difficile-G y estandarizar el valor Ct como factor predictor de grav …
Antimicrobial safety considerations in critically ill patients: part II: focused on anti-microbial toxicities
Critically ill patients who receive antimicrobial agents should be monitored for neurological, hematologic toxicities especially seizure, thrombocytopenia, and clostridioides infections. Other toxicities including QTc prolongation, electrolyte disturbances, liver enzyme elevation, and infusion-relat …
Clostridioides difficile toxin B gen amplification cycle threshold as a predictor of severity
Basándonos en los resultados de nuestro estudio, no ha sido posible la implementación sistemática del valor Ct como predictor de gravedad al informe clínico, no siendo posible extrapolar esta variable predictora de enfermedad por C difficile-G y estandarizar el valor Ct como factor predictor de grav …
Binding of the extracellular matrix laminin-1 to Clostridioides difficile strains - PubMed
These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein.
Association between Clostridium innocuum and antibiotic-associated diarrhea in adults and children: A cross-sectional study and comparative genomics analysis
C. innocuum was frequently isolated and at a greater frequency in asymptomatic controls than those with AAD. We did not identify strain lineages or accessory genomic elements associated with AAD. These data highlight that differentiating C. innocuum-associated diarrhea from asymptomatic colonization …
Do bacteria hold the key to their own defeat? - Medical News Today
Using a special algorithm, researchers found an unresistible antibiotic produced by some bacteria. This approach could advance the fight against antibiotic resistance.
Ischemic Colitis Is a Risk Factor for Clostridium difficile Infection - Cureus
Introduction: Clostridium difficile infection (CDI) is an anaerobic infection that can carry detrimental outcomes for patients and is a growing burden to the US healthcare system. Various theori...
Prior Antibiotic Exposure Is Associated With Reoperation After Elective Non-colorectal Surgery
Among Veterans, recent antibiotic exposure within 90 days of elective surgery was associated with a 39% increase in the odds of return to the operating room. Further work is needed to evaluate the effects of antibiotic exposure and dysbiosis on surgical outcomes.
Reconsidering the in vivo functions of Clostridial Stickland amino acid fermentations
Stickland amino acid fermentations occur primarily among species of Clostridia. An ancient form of metabolism, Stickland fermentations use amino acids as electron acceptors in the absence of stronger oxidizing agents and provide metabolic capabilities to support growth when other fermentable substra …
Incidence of Early and Late-Onset Clostridioides difficile Infection following Appendectomy Compared to Other Common Abdominal Surgical Procedures
The incidence of CDAD after various abdominal surgeries ranged between 2% and 7% in this study. Whereas, hemicolectomy patients had predominantly early onset CDAD, and appendectomy and cholecystectomy may increase the risk for late-onset CDAD. Appendectomy per se does not seem to increase the risk f …
Clostridioides difficile Infection in Liver Cirrhosis: A Concise Review
Clostridium difficile is a Gram-positive bacillus with fecal-oral transmission and is currently one of the most common nosocomial infections worldwide, which was renamed Clostridioides difficile in 2016. Clostridioides difficile infection (CDI) is a prevalent infection in cirrho …
Fulminant Clostridium difficile Small Bowel Enteritis in a 23-Year-Old Patient
A young female patient with a history of total colectomy developed fulminant Clostridium difficile infection of the small bowel after elective ileostomy reversal.
Rapid Quantification of C. difficile Glutamate Dehydrogenase and Toxin B (TcdB) with a NanoBiT Split-Luciferase Assay
C. difficile infection (CDI) is a leading healthcare-associated infection with a high morbidity and mortality and is a financial burden. No current standalone point-of-care test (POCT) is sufficient for the identification of true CDI over a disease-free carriage of C. difficile, so one …
The circadian immune system - Science
Clostridioides difficile infection in paediatric patients with cancer and haematopoietic stem cell transplant recipients - PubMed
The prevalence of clinical cure was 82.9% and recurrence was 9.6% in pediatric patients receiving cancer treatments. Severe CDI, as per our proposed definition, occurred in 9.7% episodes. Initial oral vancomycin was significantly associated with a reduction in repeated new CDI episodes.
Mapping of etiologies of computed tomography-proven acute colitis: a prospective cohort study - PubMed
Our objective was to describe the etiologies of acute colitis and to identify patients who require diagnostic endoscopy. Patients with symptoms of gastrointestinal infection and colonic inflammation on CT were prospectively included. Those immunosuppressed, with history of colorectal cancer or infla …
Mapping of etiologies of computed tomography-proven acute colitis: a prospective cohort study | Scientific Reports - Nature.com
Scientific Reports - Mapping of etiologies of computed tomography-proven acute colitis: a prospective cohort study
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Krishna Rao presenting biomarkers and machine learning to understand #cdiff and #CDI infection @ASMicrobiology #ASMicrobe pic.twitter.com/VoteFWX5tf— Antonella Fioravanti (@anto_fiora) June 11, 2022
The Alternative Sigma Factor SigL Influences Clostridioides difficile Toxin Production, Sporulation, and Cell Surface Properties
The alternative sigma factor SigL (Sigma-54) facilitates bacterial adaptation to the extracellular environment by modulating the expression of defined gene subsets. A homolog of the gene encoding SigL is conserved in the diarrheagenic pathogen Clostridioides difficile. To explore the contribu …
Impact of Primary and Secondary Bile Acids on Clostridioides difficile Infection
Primary bile acids (BAs), synthesized from cholesterol in the liver, after their secretion with bile into the intestinal lumen, are transformed by gut microbiota to secondary BAs. As natural detergents, BAs play a key role in the digestion and absorption of lipids and liposoluble vitamins. However, …
Structure of the glucosyltransferase domain of TcdA in complex with RhoA provides insights into substrate recognition
Clostridioides difficile is one of the most common causes of antibiotic-associated diarrhea in developed countries. As key virulence factors of C. difficile, toxin A (TcdA) and toxin B (TcdB) act by glucosylating and inactivating Rho and Ras family small GTPases in host cells, which leads to actin c …