C Diff Molecular

The progress of infection by Clostridioides difficile is strongly influenced by metabolic cues it encounters as it colonizes the gastrointestinal trac…

Antimicrobial resistance (AMR) plays an important role in the pathogenesis and spread of   Clostridioides difficile   infection (CDI), the leading healthcare-related gastrointestinal infection in the world. An association between AMR and CDI outbreaks is well documented, however, data is limited to a few ‘epidemic’ strains in specific geographical regions. Here, through detailed analysis of 10 330 publicly-available   C. difficile   genomes from strains isolated worldwide (spanning 270 multilocus sequence types (STs) across all known evolutionary clades), this study provides the first species-wide snapshot of AMR genomic epidemiology in   C. difficile  . Of the 10 330   C  .   difficile   genomes, 4532 (43.9 %) in 89 STs across clades 1–5 carried at least one genotypic AMR determinant, with 901 genomes (8.7 %) carrying AMR determinants for three or more antimicrobial classes (multidrug-resistant, MDR). No AMR genotype was identified in any strains belonging to the cryptic clades.   C. difficile   from Australia/New Zealand had the lowest AMR prevalence compared to strains from Asia, Europe and North America (P

A COMMON sugar additive used in popular foods may be the driving force behind the growth of one of the most aggressive superbugs, according to experts.

Evolution of plasmid mobility: origin and fate of non-conjugative plasmids https://t.co/WEzbeQTLCG from the @epcrocha lab. Interesting as most #cdiff #plasmids appear to be non-conjugative.— Wiep Klaas Smits (@SmitsLab) December 13, 2021

Research into the relationship between C. diff and IBD reveals inflammation creates a more conducive environment for harmful bacteria to thrive in the gut.

The bacterium Clostridioides difficile (C. diff) may be smaller than a pinhead, but it presents a massive issue for hospitals.…

Coronavirus disease 2019 (COVID-19) has rapidly spread all over the world with a very high rate of mortality. Different symptoms developed by COVID-19 infection and its impacts on various organs of the human body have highlighted the importance of both coinfections and superinfections with other pat …

Patients affected by IBD's, this infection might be facilitated by a derangement of the enteric glial cell network caused by the of proinflammatory cytokines.

The concentration of C. diff toxins in the stool was linked to how sick patients were at the time of diagnosis and was associated with adverse outcomes, including the risk of infection returning after treatment.

Shared insight on how disruption of the gut microbiome may occur based on antibiotic exposure, environmental factors, and diet.

The State of the Science events allows experts to come together to update on recent research for CDI.

Teena Chopra, MD, MPH, professor of infectious diseases at Wayne State University, discussed the role of the microbiome in overall patient health and recurrent Clostridioides difficile infection.

People who respond well to the recommended dietary therapy of restricting intake of fermentable carbs for irritable bowel syndrome, or IBS for short, have an abundance of particular types of bacteria in their gut, reveals research published online in the journal Gut.

Scientists around the world have been working in earnest to improve understanding of an increasingly virulent superbug, Clostridium difficile. The highly contagious hospital-acquired pathogen, designated ...

Teena Chopra, MD, MPH, professor of infectious diseases at Wayne State University, addressed the relationship between gut microbiome and C. diff infection.

Shared insight on the prevalence of recurring Clostridioides difficile infection and management strategies.

US biologists have conducted a study to attain unprecedented insights into Clostridium difficile, an incredibly virulent superbug.

Research into the relationship between C. diff and IBD reveals inflammation creates a more conducive environment for harmful bacteria to thrive in the gut.

Healio Gastroenterology | I hope everyone is safe and well. The delta virus peak is surging in the Upper Midwest. Hopefully we will get a bit of a reprieve in the new year. This month’s cover story touches on the potential effects of the intestinal microbiome on our immune system. It stands to reason that what we eat can affect our microbiome, and those changes in turn could impact our immune system. The cover

While the gut is typically thought of as anoxic, there are two intersecting and decreasing oxygen gradients that are observed in the gut: oxygen decreases from the small to the large intestine and from the intestinal epithelium toward the colon lumen. Gut oxygen levels also increase following antibi …

Hospitalized CDI patients with NAFLD had more intestinal complications compared to CDI patients with VLD and ALD. Gut microbiota dysbiosis may contribute to the pathogenesis of intestinal complications.

As an anaerobe, Clostridioides difficile relies on the formation of a dormant spore for survival outside of the mammalian host's gastrointestinal tract. The spore is recalcitrant to desiccation, numerous disinfectants, UV light, and antibiotics, permitting long-term survival against environmental in …

Clostridioides difficile is an opportunistic pathogen that causes by a high rate of recurrent infections. Persistence in the gastrointestinal tract is thought to be mediated by sporulation and/or biofilm formation. There is an increase interest in C. difficile biofilm formation and recent findings h …

[This corrects the article DOI: 10.3389/fimmu.2021.691039.].

Clostridioides difficile (formerly Clostridium difficile)-associated diarrhea is currently the most frequently occurring nosocomial diarrhea worldwide. During its infection cycle this pathogen needs to survive in phage-rich gut communities. Recent data strongly suggest that regulatory RNAs control g …

Despite appropriate infection control strategies in the burn intensive care unit, CDI remains prevalent in severe burn patients. Eventually, the overgrowth of A. muciniphila and the decreased abundance of F. prausnitzii in burn cases with CDI could be potential predictive microbiome biomarkers in bu …

CDI causes significant morbidity in patients with malignancies. A high qSOFA score and leukocytosis are significantly associated with high morbidity and thus should be used to prioritize and intensify inpatient care of these patients.

Clostridioides difficile is often resistant to the actions of antibiotics to treat other bacterial infections and the resulting C. difficile infection (CDI) is among the leading causes of nosocomial infectious diarrhea worldwide. The primary virulence mechanism contributing to CDI is the production of toxins. Treatment failures and recurrence of CDI have urged the medical community to search for novel treatment options. Strains that do not produce toxins, so called non-toxigenic C. difficile, have been known to colonize the colon and protect the host against CDI. In this review, a comprehensive description and comparison of the immune responses to toxigenic C. difficile and non-toxigenic adherence, and colonization factors, here called non-toxin proteins, is provided. This revealed a number of similarities between the host immune responses to toxigenic C. difficile and non-toxin proteins, such as the influx of granulocytes and the type of T-cell response. Differences may reflect genuine variation between the responses to toxigenic or non-toxigenic C. difficile or gaps in the current knowledge with respect to the immune response toward non-toxigenic C. difficile. Toxin-based and non-toxin-based immunization studies have been evaluated to further explore the role of B cells and reveal that plasma cells are important in protection against CDI. Since the success of toxin-based interventions in humans to date is limited, it is vital that future research will focus on the immune...

Clostridioides difficile (C. difficile or C. diff) is a bacterium that is normally present in the body in small numbers. However, disruption of the body’s