C Diff Molecular

C Diff Molecular

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Clostridioides difficile infection in solid organ and hematopoietic stem cell transplant recipients: A prospective multinational study
Clostridioides difficile infection in solid organ and hematopoietic stem cell transplant recipients: A prospective multinational study
Background Clostridioides difficile infection (CDI) is a significant cause of morbidity and mortality in recipients of solid organ transplant (SOT) or hematopoietic stem cell transplant (HSCT). In r...
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Clostridioides difficile infection in solid organ and hematopoietic stem cell transplant recipients: A prospective multinational study
Gut: Opinions vary on potential effect on immune system - Healio
Gut: Opinions vary on potential effect on immune system - Healio
Healio Gastroenterology | I hope everyone is safe and well. The delta virus peak is surging in the Upper Midwest. Hopefully we will get a bit of a reprieve in the new year. This month’s cover story touches on the potential effects of the intestinal microbiome on our immune system. It stands to reason that what we eat can affect our microbiome, and those changes in turn could impact our immune system. The cover
·healio.com·
Gut: Opinions vary on potential effect on immune system - Healio
Impairment of lysosomal function by Clostridioides difficile TcdB
Impairment of lysosomal function by Clostridioides difficile TcdB
TcdB is a potent cytotoxin produced by pathogenic Clostridioides difficile that inhibits Rho GTPases by mono-glucosylation. TcdB enters cells via receptor-mediated endocytosis. The pathogenic glucosyltransferase domain (GTD) egresses endosomes by pH-mediated conformational changes, and is subsequent …
·pubmed.ncbi.nlm.nih.gov·
Impairment of lysosomal function by Clostridioides difficile TcdB
Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice
Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice
Interaction of Clostridioides difficile spores with the intestinal mucosa contributes to the persistence and recurrence of the infection. Advanced age is one of the main risk factors for C. difficile infection and recurrence of the disease. However, interaction of C. difficile spores with the intest …
·pubmed.ncbi.nlm.nih.gov·
Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice
Clostridioides difficile toxins: mechanisms of action and antitoxin therapeutics
Clostridioides difficile toxins: mechanisms of action and antitoxin therapeutics
Clostridioides difficile is a Gram-positive anaerobe that can cause a spectrum of disorders that range in severity from mild diarrhoea to fulminant colitis and/or death. The bacterium produces up to three toxins, which are considered the major virulence factors in C. difficile infection. These toxin …
·pubmed.ncbi.nlm.nih.gov·
Clostridioides difficile toxins: mechanisms of action and antitoxin therapeutics
What's metal got to do with it? Transition metals in Clostridioides difficile infection
What's metal got to do with it? Transition metals in Clostridioides difficile infection
The enteric pathogen Clostridioides difficile overcomes barriers to colonization imposed by the microbiota and host immune response to induce disease. To navigate the dynamic gut environment, C. difficile must respond to dietary and host-mediated fluctuations in transition metal availability. Transi …
·pubmed.ncbi.nlm.nih.gov·
What's metal got to do with it? Transition metals in Clostridioides difficile infection
Phylogenetic analysis of the bacterial Pro-Pro-endopeptidase domain reveals a diverse family including secreted and membrane anchored proteins
Phylogenetic analysis of the bacterial Pro-Pro-endopeptidase domain reveals a diverse family including secreted and membrane anchored proteins
Pro-Pro-endopeptidases (PPEP, EC 3.4.24.89) are secreted, zinc metalloproteases that have the unusual capacity to cleave a peptide bond between two prolines, a bond that is generally less sensitive to proteolytic cleavage. Two well studied members of the family are PPEP-1 and PPEP-2, produced by …
·pubmed.ncbi.nlm.nih.gov·
Phylogenetic analysis of the bacterial Pro-Pro-endopeptidase domain reveals a diverse family including secreted and membrane anchored proteins
Association of body mass index with Clostridioides difficile infection among older patients with pneumonia in Japan
Association of body mass index with Clostridioides difficile infection among older patients with pneumonia in Japan
Body mass index was associated with C. difficile infection in older pneumonia patients in Japan. Underweight was a risk factor, whereas overweight was a protective factor for C. difficile infection. Geriatr Gerontol Int 2021; ••: ••-••.
·pubmed.ncbi.nlm.nih.gov·
Association of body mass index with Clostridioides difficile infection among older patients with pneumonia in Japan
Regulation of para-cresol production in Clostridioides difficile
Regulation of para-cresol production in Clostridioides difficile
The human pathogen Clostridioides difficile colonises the gastrointestinal tract following antibiotic exposure, which causes perturbations in the beneficial microbiome. An unusual feature of C. difficile among the gut microbiota is its ability to produce high concentrations of the antimicrobial comp …
·pubmed.ncbi.nlm.nih.gov·
Regulation of para-cresol production in Clostridioides difficile
Receptor binding mechanisms of Clostridioides difficile toxin B and implications for therapeutics development
Receptor binding mechanisms of Clostridioides difficile toxin B and implications for therapeutics development
Clostridioides difficile is classified as an urgent antibiotic resistance threat by the Centers for Disease Control and Prevention (CDC). C. difficile infection (CDI) is mainly caused by the C. difficile exotoxin TcdB, which invades host cells via receptor-mediated endocytosis. However, many natural …
·pubmed.ncbi.nlm.nih.gov·
Receptor binding mechanisms of Clostridioides difficile toxin B and implications for therapeutics development
Second messenger signaling in Clostridioides difficile
Second messenger signaling in Clostridioides difficile
Small, diffusible second messenger molecules transmit information about extracellular conditions to intracellular machinery in order to influence transcription, translation, and metabolism. The enteropathogenic bacterium Clostridioides difficile coordinates its response to a dynamic and hostile envi …
·pubmed.ncbi.nlm.nih.gov·
Second messenger signaling in Clostridioides difficile
Clostridioides difficile infection in solid organ and hematopoietic stem cell transplant recipients: A prospective multinational study
Clostridioides difficile infection in solid organ and hematopoietic stem cell transplant recipients: A prospective multinational study
Among adults who develop CDI after SOT or HSCT, despite their immunosuppressed state, the percentage with clinical cure was high and the percentage with recurrence was low. Clinical cure and recurrence varied by type of initial treatment, and CMV viremia/disease was associated with an increased risk …
·pubmed.ncbi.nlm.nih.gov·
Clostridioides difficile infection in solid organ and hematopoietic stem cell transplant recipients: A prospective multinational study
Infection with Clostridioides difficile ribotype 046 in a paediatric liver transplant patient
Infection with Clostridioides difficile ribotype 046 in a paediatric liver transplant patient
Clostridioides difficile causes nosocomial diarrhoea associated with antibiotic use and immunodeficiency. Although the number of paediatric C. difficile infections (CDIs) has increased worldwide, there are few studies on the molecular characterization of strains causing CDIs among chil …
·pubmed.ncbi.nlm.nih.gov·
Infection with Clostridioides difficile ribotype 046 in a paediatric liver transplant patient
High-resolution structure of native toxin A from Clostridioides difficile
High-resolution structure of native toxin A from Clostridioides difficile
Clostridioides difficile infections have emerged as the leading cause of healthcare-associated infectious diarrhea. Disease symptoms are mainly caused by the virulence factors, TcdA and TcdB, which are large homologous multidomain proteins. Here, we report a 2.8 Å resolution cryo-EM structure of nat …
·pubmed.ncbi.nlm.nih.gov·
High-resolution structure of native toxin A from Clostridioides difficile
Clostridioides difficile spore germination: initiation to DPA release
Clostridioides difficile spore germination: initiation to DPA release
Germination by Clostridioides difficile spores is an essential step in pathogenesis. Spores are metabolically dormant forms of bacteria that resist severe conditions. Work over the last 10 years has elucidated that C. difficile spores germinate thorough a novel pathway. This review summarizes our un …
·pubmed.ncbi.nlm.nih.gov·
Clostridioides difficile spore germination: initiation to DPA release
Serum procalcitonin levels associate with Clostridioides difficile infection in patients with inflammatory bowel disease - PubMed
Serum procalcitonin levels associate with Clostridioides difficile infection in patients with inflammatory bowel disease - PubMed
Our results indicate that procalcitonin level can be a good candidate biomarker for assessing the CDI in IBD patients. Further studies are required to decipher whether procalcitonin can predict CDI therapy or its recurrence.
·pubmed.ncbi.nlm.nih.gov·
Serum procalcitonin levels associate with Clostridioides difficile infection in patients with inflammatory bowel disease - PubMed
Three orphan histidine kinases inhibit Clostridioides difficile sporulation
Three orphan histidine kinases inhibit Clostridioides difficile sporulation
The ability of the anaerobic gastrointestinal pathogen, Clostridioides difficile , to survive outside the host relies on the formation of dormant endospores. Spore formation is contingent on the activation of a conserved transcription factor, Spo0A, by phosphorylation. Multiple kinases and phosphatases regulate Spo0A activity in other spore-forming organisms; however, these factors are not well conserved in C. difficile . Previously, we discovered that deletion of a conserved phosphotransfer protein, CD1492, increases sporulation, indicating that CD1492 inhibits C. difficile spore formation. In this study, we investigate the functions of additional conserved orphan phosphotransfer proteins, CD2492, CD1579, and CD1949 which are hypothesized to regulate Spo0A phosphorylation. Disruption of the conserved phosphotransfer protein, CD2492, also increased sporulation frequency, similarly to the CD1492 mutant, and in contrast to a previous study. A CD1492 CD2492 mutant phenocopied the sporulation and gene expression patterns of the single mutants, suggesting that these proteins function in the same genetic pathway to repress sporulation. Deletion of the conserved CD1579 phosphotransfer protein also variably increased sporulation frequency; however, knockdown of CD1949 expression did not influence sporulation. We provide evidence that CD1492, CD2492 and CD1579 function as phosphatases, as mutation of the conserved histidine residue for phosphate transfer abolished CD2492 function, and expression of the CD1492 or CD2492 histidine site-directed mutants or the wild-type CD1579 allele in a parent strain resulted in a dominant negative hypersporulation phenotype. Altogether, at least three phosphotransfer proteins, CD1492, CD2492 and CD1579 (herein, PtpA, PtpB and PtpC) repress C. difficile sporulation initiation by regulating activity of Spo0A. ### Competing Interest Statement The authors have declared no competing interest.
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Three orphan histidine kinases inhibit Clostridioides difficile sporulation