C Diff Molecular

C Diff Molecular

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Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans - Science Advances
Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans - Science Advances
The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live animals, as well as for the assessment of BSH activity in the entire gas...
·advances.sciencemag.org·
Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans - Science Advances
Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans - Science Advances
Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans - Science Advances
The microbiome-produced enzyme bile salt hydrolase (BSH) plays a central role in human health, but its function remains unclear due to the lack of suitable methods for measuring its activity. Here, we have developed a novel optical tool based on ultrasensitive bioluminescent imaging and demonstrated that this assay can be used for quick and cost-effective quantification of BSH activity across a broad range of biological settings including pure enzymes and bacteria, intact fecal slurries, and noninvasive imaging in live animals, as well as for the assessment of BSH activity in the entire gas...
·advances.sciencemag.org·
Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans - Science Advances
Inflammatory Bowel Disease Presenting With Concurrent COVID-19 Multisystem Inflammatory Syndrome - American Academy of Pediatrics
Inflammatory Bowel Disease Presenting With Concurrent COVID-19 Multisystem Inflammatory Syndrome - American Academy of Pediatrics
Coronavirus disease 2019 is associated with a postinfectious multisystem inflammatory syndrome in children (MIS-C). This syndrome is marked by cytokine storm and multiorgan dysfunction, often affecting the gastrointestinal tract, the heart, and the hematopoietic system. We describe the case of a 16-year-old boy with an initial presentation of severe inflammatory bowel disease and concurrent MIS-C. He presented with abdominal pain, diarrhea, and hematochezia and met criteria for the systemic inflammatory response syndrome. Laboratory inflammatory profiling revealed markedly elevated ferritin...
·pediatrics.aappublications.org·
Inflammatory Bowel Disease Presenting With Concurrent COVID-19 Multisystem Inflammatory Syndrome - American Academy of Pediatrics
Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks
Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks
Clostridioides difficile is the single most deadly bacterial pathogen in the United States, and its global prevalence and outsized health impacts underscore the need for more effective therapeutic options. Towards this goal, a novel group of modified peptidoglycan hydrolases with significant in vitr …
·pubmed.ncbi.nlm.nih.gov·
Bioinformatics-Driven Discovery of Novel Clostridioides difficile Lysins and Experimental Comparison with Highly Active Benchmarks
Gut microbiome and Clostridioides difficile infection: a closer look at the microscopic interface
Gut microbiome and Clostridioides difficile infection: a closer look at the microscopic interface
The pathogenesis of Clostridioides difficile infection (CDI) was recognized with its link to the use of antimicrobials. Antimicrobials significantly alter gut microbiota structure and composition, which led to the discovery of the association of this gut perturbation with the development of C …
·pubmed.ncbi.nlm.nih.gov·
Gut microbiome and Clostridioides difficile infection: a closer look at the microscopic interface
The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb
The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb
Clostridioides (C.) difficile is clinically highly relevant and produces several AB-type protein toxins, which are the causative agents for C. difficile-associated diarrhea and pseudomembranous colitis. Treatment with antibiotics can lead to C. difficile overgrowth in the gut of patients due to the …
·pubmed.ncbi.nlm.nih.gov·
The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb
Characteristics of Clostridioides difficile colonization in Ilamian children
Characteristics of Clostridioides difficile colonization in Ilamian children
The colonization rates of C. difficile and toxigenic C. difficile were 10% (8/80) and 3.75% (3/80), respectively. Based on the age group, the intestinal-carriage rates of C. difficile were 37.5, 50, and 12.5% in children ≤ 1, 1-3, and 3-5 years old, respectively. Our findings have revealed eight dis …
·pubmed.ncbi.nlm.nih.gov·
Characteristics of Clostridioides difficile colonization in Ilamian children
Gut microbiota profiles in diarrheic patients with co-occurrence of Clostridioides difficile and Blastocystis
Gut microbiota profiles in diarrheic patients with co-occurrence of Clostridioides difficile and Blastocystis
Blastocystis and Clostridioides difficile co-occurrence is considered a rare event since the colonization by Blastocystis is prevented under a decrease in beneficial bacteria in the microbiota when there is C. difficile infection (CDI). This scenario has been reported once, but no information on the …
·pubmed.ncbi.nlm.nih.gov·
Gut microbiota profiles in diarrheic patients with co-occurrence of Clostridioides difficile and Blastocystis
Leptin Receptor q223r Polymorphism Influences Clostridioides difficile Infection-Induced Neutrophil CXCR2 Expression in an Interleukin-1beta Dependent Manner
Leptin Receptor q223r Polymorphism Influences Clostridioides difficile Infection-Induced Neutrophil CXCR2 Expression in an Interleukin-1beta Dependent Manner
Neutrophils are key first-responders in the innate immune response to C. difficile infection (CDI) and play a central role in disease pathogenesis. Studies have clearly shown that tissue neutrophil numbers need to be tightly regulated for optimal CDI outcomes: while excessive colonic neutroph …
·pubmed.ncbi.nlm.nih.gov·
Leptin Receptor q223r Polymorphism Influences Clostridioides difficile Infection-Induced Neutrophil CXCR2 Expression in an Interleukin-1beta Dependent Manner
Microbiological features epidemiology and clinical presentation of Clostridioides difficile strains from MLST Clade 2: a narrative review
Microbiological features epidemiology and clinical presentation of Clostridioides difficile strains from MLST Clade 2: a narrative review
Clostridioides difficile is an emerging One Health pathogen and a common etiologic agent of diarrhea, both in healthcare settings and the community. This bacterial species is highly diverse, and its global population has been classified in eight clades by multilocus sequence typing (MLST). The C. di …
·pubmed.ncbi.nlm.nih.gov·
Microbiological features epidemiology and clinical presentation of Clostridioides difficile strains from MLST Clade 2: a narrative review
The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb
The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb
Clostridioides (C.) difficile is clinically highly relevant and produces several AB-type protein toxins, which are the causative agents for C. difficile-associated diarrhea and pseudomembranous colitis. Treatment with antibiotics can lead to C. difficile overgrowth in the gut of patients due to the …
·pubmed.ncbi.nlm.nih.gov·
The cytotoxic effect of Clostridioides difficile pore-forming toxin CDTb
New ribotype Clostridioides difficile from ST11 group revealed higher pathogenic ability than RT078
New ribotype Clostridioides difficile from ST11 group revealed higher pathogenic ability than RT078
Clostridioides difficile is the predominant antibiotic-associated enteropathogen associated with diarrhoea or pseudomembranous colitis in patients worldwide. Previously, we identified C. difficile RT078 isolates (CD21062) from elderly patients in China, including two new ribotype strai …
·pubmed.ncbi.nlm.nih.gov·
New ribotype Clostridioides difficile from ST11 group revealed higher pathogenic ability than RT078
The high prevalence of Clostridioides difficile among nursing home elders associates with a dysbiotic microbiome
The high prevalence of Clostridioides difficile among nursing home elders associates with a dysbiotic microbiome
Clostridioides difficile disproportionally affects the elderly living in nursing homes (NHs). Our objective was to explore the prevalence of C. difficile in NH elders, over time and to determine whether the microbiome or other clinical factors are associated with C. difficile co …
·pubmed.ncbi.nlm.nih.gov·
The high prevalence of Clostridioides difficile among nursing home elders associates with a dysbiotic microbiome
Fecal Mycobiota Combined with Host Immune Factors Distinguish Clostridioides difficile Infection from Asymptomatic Carriage
Fecal Mycobiota Combined with Host Immune Factors Distinguish Clostridioides difficile Infection from Asymptomatic Carriage
Our study provides specific markers of stool fungi combined with host immune factors to distinguish CDI patients from Carrier. It highlights the importance of gut mycobiome in CDI, which may have been underestimated. Further studies on the diagnostic applications and therapeutic potentials of these …
·pubmed.ncbi.nlm.nih.gov·
Fecal Mycobiota Combined with Host Immune Factors Distinguish Clostridioides difficile Infection from Asymptomatic Carriage
Contribution of Inhibitory Metabolites and Competition for Nutrients to Colonization Resistance against Clostridioides difficile by Commensal Clostridium
Contribution of Inhibitory Metabolites and Competition for Nutrients to Colonization Resistance against Clostridioides difficile by Commensal Clostridium
Clostridioides difficile is an anaerobic pathogen that causes significant morbidity and mortality. Understanding the mechanisms of colonization resistance against C. difficile is important for elucidating the mechanisms by which C. difficile is able to colonize the gut after ant …
·pubmed.ncbi.nlm.nih.gov·
Contribution of Inhibitory Metabolites and Competition for Nutrients to Colonization Resistance against Clostridioides difficile by Commensal Clostridium
A Point Mutation in the Transcriptional Repressor PerR Results in a Constitutive Oxidative Stress Response in Clostridioides difficile 630deltaerm
A Point Mutation in the Transcriptional Repressor PerR Results in a Constitutive Oxidative Stress Response in Clostridioides difficile 630deltaerm
The human pathogen Clostridioides difficile has evolved into the leading cause of nosocomial diarrhea. The bacterium is capable of spore formation, which even allows survival of antibiotic treatment. Although C. difficile features an anaerobic lifestyle, we determined a remarkably high …
·pubmed.ncbi.nlm.nih.gov·
A Point Mutation in the Transcriptional Repressor PerR Results in a Constitutive Oxidative Stress Response in Clostridioides difficile 630deltaerm
The State of Microbiome Science at the Intersection of Infectious Diseases and Antimicrobial Resistance
The State of Microbiome Science at the Intersection of Infectious Diseases and Antimicrobial Resistance
Along with the rise in modern chronic diseases, ranging from diabetes to asthma, lay the challenges posed by increasing antibiotic resistance resulting in difficult to treat infections, as well as sepsis. An emerging and unifying theme in the pathogenesis of these diverse public health threats is ch …
·pubmed.ncbi.nlm.nih.gov·
The State of Microbiome Science at the Intersection of Infectious Diseases and Antimicrobial Resistance
Predictive factors outcomes and molecular epidemiology of Clostridioides difficile diarrhea in Brazilian hospitals
Predictive factors outcomes and molecular epidemiology of Clostridioides difficile diarrhea in Brazilian hospitals
Little is known about the role of lineage of strains of Clostridioides difficile (CD) on the clinical presentation of CD infection (CDI) in Latin America, especially regarding the treatment response. We conducted a multicenter, prospective study to investigate the predictive factors and treatment ou …
·pubmed.ncbi.nlm.nih.gov·
Predictive factors outcomes and molecular epidemiology of Clostridioides difficile diarrhea in Brazilian hospitals
Novel risk factors and outcomes in inflammatory bowel disease patients with Clostridioides difficile infection
Novel risk factors and outcomes in inflammatory bowel disease patients with Clostridioides difficile infection
An overweight BMI and statin use were associated with severe or severe, complicated CDI in IBD patients. Further studies are needed to better understand how these factors impact management of patients with IBD to improve clinical outcomes and potentially reduce the risk of complications from CDI.
·pubmed.ncbi.nlm.nih.gov·
Novel risk factors and outcomes in inflammatory bowel disease patients with Clostridioides difficile infection
Building blocks and blueprints for bacterial autolysins
Building blocks and blueprints for bacterial autolysins
Bacteria utilize a wide variety of endogenous cell wall hydrolases, or autolysins, to remodel their cell walls during processes including cell division, biofilm formation, and programmed death. We here systematically investigate the composition of these enzymes in order to gain insights into their a …
·pubmed.ncbi.nlm.nih.gov·
Building blocks and blueprints for bacterial autolysins
Ser/Thr Kinase-Dependent Phosphorylation of the Peptidoglycan Hydrolase CwlA Controls Its Export and Modulates Cell Division in Clostridioides difficile
Ser/Thr Kinase-Dependent Phosphorylation of the Peptidoglycan Hydrolase CwlA Controls Its Export and Modulates Cell Division in Clostridioides difficile
Cell growth and division require a balance between synthesis and hydrolysis of the peptidoglycan (PG). Inhibition of PG synthesis or uncontrolled PG hydrolysis can be lethal for the cells, making it imperative to control peptidoglycan hydrolase (PGH) activity. The synthesis or activity of several ke …
·pubmed.ncbi.nlm.nih.gov·
Ser/Thr Kinase-Dependent Phosphorylation of the Peptidoglycan Hydrolase CwlA Controls Its Export and Modulates Cell Division in Clostridioides difficile
Immune response against Clostridioides difficile and translation to therapy
Immune response against Clostridioides difficile and translation to therapy
The pathogenesis of Clostridioides difficile infection (CDI) has largely been attributed to the action of two major toxins - A and B. An enhanced systemic humoral immune response against these toxins has been shown to be protective against recurrent CDI. Over the years, fully human monoclonal …
·pubmed.ncbi.nlm.nih.gov·
Immune response against Clostridioides difficile and translation to therapy