2016

C-diff is a bacterium that causes a life-threatening infection. Though the bacterium can infect healthy individuals, it is of particular concern to those who are hospitalized or are taking

Abstract. Background. Clostridium difficile is a primary cause of antibiotic-associated diarrhea that typically develops when gut microbiota is altered. Convent

Fidaxomicin has been scrutinized because of its high acquisition cost. Real-world experience is needed to determine whether fidaxomicin has value in patients with Clostridium difficile–associated diarrhea (CDAD) and certain risk factors.In this ...

c ribotypes of C. difficile harbour mutations or have acquired extra genes that mean these strains can utilize lower concentrations of bioavailable trehalose, providing a competitive metabolic advantage in some CDI animal models. By contrast, evidence has emerged to show that trehalose-induced microbiota changes can help protect/reduce CDI in other models. In addition, C. difficile trehalose metabolic variants are widespread among epidemic and nonepidemic ribotypes alike, and the occurrence of these trehalose variants was not associated with increase disease severity or mortality. Summary Currently, there is no proven causal association between the incidence or severity of human CDI and the presence of trehalose metabolism variants. Furthermore, microbial metabolism reduces trehalose bioavailability, potentially removing this competitive advantage for C. difficile trehalose metabolism variants. Taken together, trehalose consumed as part of a normal diet has no increased risk of CDI....

What is fidaxomicin? (Dificid/Dificlr) Why is it effective against C Difficile? Find out more about the drug and the disease here

The consumption of dietary supplements and cold therapies containing high concentrations of zinc is now being called into question, following research that suggests it may worsen Clostridium difficile infection.

The increasing frequency and severity of healthcare-associated outbreaks caused by bacterium Clostridium difficile have been linked to the widely used...

This Medical News article discusses whether trehalose, a sugar manufactured from cornstarch used to sweeten and texturize foods, has contributed to the emergence of epidemic strains of Clostridium difficile infection (CDI).

Two hypervirulent ribotypes of the enteric pathogen Clostridium difficile, RT027 and RT078, have independently acquired unique mechanisms to metabolize low concentrations of the disaccharide trehalose, suggesting a correlation between the emergence of these ribotypes and the widespread adoption of trehalose in the human diet.

National Institute for Health Research. Gut model experiments only: Hayashibara Co. Ltd.

Clostridium difficile disease has recently increased to become a dominant nosocomial pathogen in North America and Europe, although little is known about what has driven this emergence. Here we show that two epidemic ribotypes (RT027 and RT078) have acquired unique mechanisms to metabolize low conce …

C.Difficile sickens 500,000 every year. It contributes to the death of up to 30,000. Discover what you can do to prevent, treat and understand c diff

Bile acids, which are altered by bacteria normally living in the large intestine, inhibit the growth of Clostridium difficile, new research indicates. The work sheds light on the ways in which some commonly used antibiotics can promote C. diff infections by killing off the bile acid-altering microbes.

Abstract. In a case–control study of patients with Clostridium difficile infection, we found no statistically significant association between the presence of tr

Rough notes from the webinar The Scientist: Precision Medicine Learning Lessons from the Microbiome (now available online)

Preservation of blood plasma in the dried state would facilitate long-term storage and transport at ambient temperatures, without the need of to use liquid nitrogen tanks or freezers. The aim of this study was to investigate the feasibility of dry preservation of human plasma, using sugars as lyopro …

Learn more about this infectious disease that is becoming more common and often affects older adults in a health care facility, often after antibiotic use.

Learn about the Novavax Coronavirus vaccine, designed to protect against COVID-19. This vaccine is now in Phase 3 clinical studies to evaluate its efficacy.

Deaths and Adverse Effects from the Covid Vaccines, Just Aniother Vioxx ?

Is the world at risk? Experts debate the issue. https://www.mcgill.ca/oss/article/covid-19-critical-thinking-pseudoscience/doomsday-prophecy-dr-geert-vanden-bossche https://www.geertvandenbossche.org/ Dark Horse Interview https://www.youtube.com/watch?v=BNyAovuUxro

Since the start of the COVID-19 outbreak, the race for testing new platforms designed to confer immunity against SARS-CoV-2, has been rampant and unprecedented, leading to conditional emergency authorization of various vaccines. Despite progress on ea

Prolonged SARS-CoV-2 RNA shedding and recurrence of PCR-positive tests have been widely reported in patients after recovery, yet these patients most commonly are non-infectious[1][1]–[14][2]. Here we investigated the possibility that SARS-CoV-2 RNAs can be reverse-transcribed and integrated into the human genome and that transcription of the integrated sequences might account for PCR-positive tests. In support of this hypothesis, we found chimeric transcripts consisting of viral fused to cellular sequences in published data sets of SARS-CoV-2 infected cultured cells and primary cells of patients, consistent with the transcription of viral sequences integrated into the genome. To experimentally corroborate the possibility of viral retro-integration, we describe evidence that SARS-CoV-2 RNAs can be reverse transcribed in human cells by reverse transcriptase (RT) from LINE-1 elements or by HIV-1 RT, and that these DNA sequences can be integrated into the cell genome and subsequently be transcribed. Human endogenous LINE-1 expression was induced upon SARS-CoV-2 infection or by cytokine exposure in cultured cells, suggesting a molecular mechanism for SARS-CoV-2 retro-integration in patients. This novel feature of SARS-CoV-2 infection may explain why patients can continue to produce viral RNA after recovery and suggests a new aspect of RNA virus replication. ### Competing Interest Statement The authors have declared no competing interest. [1]: #ref-1 [2]: #ref-14

“They Don’t Want to See People Like Us” In a Highwire exclusive, Del sits down with three healthcare workers who were on the frontline of Covid vaccine rollouts in America. In a candid and emotional interview, the three women go back to the day they received their vaccine, the severe reactions they endured starting just… Read More »“THEY DON’T WANT TO SEE PEOPLE LIKE US”

There have been many thousands of friendly fire incidents in recorded military history, accounting for an estimated 2% to 20% of all casualties in battle. Each case is a horror story, a tragedy. With vaccines, it is similar. No matter how certified safe vaccines are, there are always vaccine damages and death, which in America are all... View Article