2016

Background Glucosamine hydrochloride, a natural biopolymer present in the daily diet, has various biological activities including antitumour properties and protective effects against pathogens. Early studies showed that daily administration of a derivative of glucosamine induced proliferation of leukemia cells and prolonged overall survival in mice; importantly, no toxicity was associated with the glucosamine treatment. However, the potential mechanism of the antitumour effect is unknown. This study aimed to investigate the inhibitory mechanism and effect of glucosamine on human gastric carcinoma cells in vitro. Methods Gastric carcinoma MKN-45 cells were exposed to 0, 100, 500 and 1000 µg/mL glucosamine hydrochloride for 72 hours, and then the viability and proliferation of gastric carcinoma cells in vitro was measured using the MTT (3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay. Quantitative gene expression of MMP-2 and -3 was determined by real-time polymerase chain reaction. Protein level was analyzed using an enzyme-linked immunosorbent assay (ELISA). Results Glucosamine hydrochloride has a significant inhibitory antitumour effect on MKN-45 cells in vitro. The cell viability of MKN-45 cells treated with different concentrations of glucosamine hydrochloride rose continuously from 24 to 72 hours compared with the untreated control. MKN-45 cells were inhibited by 54% by 500 µg/mL glucosamine hydrochloride and by 85% by 1000 µg/mL glucosamine hydrochloride. Administration of 500 µg/mL glucosamine hydrochloride resulted in a significant decrease in MMP-2 and MMP-3 expression of about 79% and 70%, respectively, in MKN-45 cells. Conclusions In this study, we showed that the antitumour activity of glucosamine hydrochloride significantly suppresses MKN-45 cells in vitro. This effect was shown by inhibitory gene expression of MMP-2 and -3. Acknowledgments This research was financially supported by Zhuhai College of Jilin University.

It is well established that trehalose (TRH) affects the physical properties of lipid bilayers and stabilizes biological membranes. We present molecular dynamics (MD) computer simulations to investigate the interactions between lipid membranes formed by 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and TRH. Both atomistic and coarse-grained (CG) interaction models were employed, and the coarse graining of DMPC leads to a reduction in the acyl chain length corresponding to a 1,2-dilauroyl-sn-glycero-3-phosphocholine lipid (DLPC). Several modifications of the Martini interaction model, used for CG simulations, were implemented, resulting in different potentials of mean force (PMFs) for DMPC bilayer–TRH interactions. These PMFs were subsequently used in a simple two-site analytical model for the description of sugar binding at the membrane interface. In contrast to that in atomistic MD simulations, the binding in the CG model was not in agreement with the two-site model. Our interpretation is that the interaction balance, involving water, TRH, and lipids, in the CG systems needs further tuning of the force-field parameters. The area per lipid is only weakly affected by TRH concentration, whereas the compressibility modulus related to the fluctuations of the membrane increases with an increase in TRH content. In agreement with experimental findings, the bending modulus is not affected by the inclusion of TRH. The important aspects of lipid bilayer interactions with biomolecules are membrane curvature generation and sensing. In the present investigation, membrane curvature is generated by artificial buckling of the bilayer in one dimension. It turns out that TRH prefers the regions with the highest curvature, which enables the most favorable situation for lipid–sugar interactions.

Ulcerative colitis (UC), an idiopathic inflammatory disorder in the colon, has become a clinical challenge, owing to the increasing incidence and poor prognosis. The conventional treatments for UC including aminosalicylates, corticosteroids, and immunosuppressants, ...

Information and research on acemannan.

Aggregation of proteins is a physiological process which contributes to the pathophysiology of several maladies including diabetes mellitus, Huntingto…

The nutraceutical properties of Aloe vera have been attributed to a glucomannan known as acemannan. Recently information has been published about the presence of fructans in Aloe vera but there are no publications about acemannan and fructans as prebiotic compounds. This study investigated in vitro the prebiotic properties of these polysaccharides. Our results demonstrated that fructans from Aloe vera induced bacterial growth better than inulin (commercial FOS). Acemannan stimulated bacterial growth less than fructans, and as much as commercial FOS. Using qPCR to study the bacterial population of human feces fermented in a bioreactor simulating colon conditions, we found that fructans induce an increase in the population of Bifidobacterium spp. Fructans produced greater amounts of short chain fatty acids (SCFA), while the branched-chain fatty acids (BCFA) did not increase with these polysaccharides. Acemannan increased significantly acetate concentrations. Therefore, both Aloe vera polysaccharides have prebiotic potentials.

The aim of present work was to investigate preventive role of orally administered Aloe vera supplemented probiotic lassi (APL) on Shigella dysenteriae…

Several factors limit the absorption and bioavailability of vitamins. Vitamin C, a commonly used water-soluble supplement reduces the risk of disease. Vitamin B(12) is necessary for the development of RBC, growth, and nervous system. Vitamin B(12) deficiency is common among elderly. Thus, agents tha …

Reactive oxygen species (ROS) are implicated in the aetiology of gastric disorders and are a key pathogenic feature in gastric mucosal injury. Interve…

This study seems to suggest that Aloe may be successfully associated with chemotherapy to increase its efficacy in terms of both tumor regression rate and survival time.

Clinicians and wound care nurses in Indonesia usually use Nigella sativa oil (NSO) gel and aloe vera (AV) gel to treat diabetic ulcers. However, there are no st...

Objective Corrosion Reviews is an international bimonthly journal devoted to critical reviews and, to a lesser extent, outstanding original articles that are key to advancing the understanding and application of corrosion science and engineering in the service of society. Papers may be of a theoretical, experimental or practical nature, provided that they make a significant contribution to knowledge in the field. Topics Cutting-edge topics such as but not limited to the following: Corrosion of materials in energy systems Corrosion of biomaterials and medical devices Corrosion of materials in the chemical process industries High temperature corrosion Nuclear waste disposal The effect of corrosion on water pollution and the environment Corrosion of advanced materials Degradation of non-metals Corrosion control and monitoring techniques Modeling and life prediction Article formats Review articles and high-relevant original articles > Information on submission process

Clinicians and wound care nurses in Indonesia usually use Nigella sativa oil (NSO) gel and aloe vera (AV) gel to treat diabetic ulcers. However, there are no st...

As we age our eyes gradually cloud over, with unchecked cataracts the leading cause of blindness. Here are 4 simple things you can do to make sure your eyes stay youthful late into life

The medical profession tells us that surgery is the only option for removing cataracts. While surgery may be the best option for treating an advanced case of cataracts ...

According to the World Health Organization, about 42% of all blindness is due to cataracts. A cataract forms when the lens behind the colored part of your eye becomes cloudy. Degradation of eye tissue can reduce the clarity of images when the tissue clumps together. Over time, the thickness of the clumped tissue increases and can lead to partial or complete blindness. While there are several types of cataracts, each type shares many of the same causes. I've been receiving a lot of questions about cataracts, and the three big questions I will address today are: When is it time to do cataract surgery? What is the best lens to put in your eye? What can you do post-surgically to improve your eye health? 🔴SUBSCRIBE to my channel for more holistic approaches to eye health!🔴 https://www.youtube.com/sberneod To learn more about how you may address the causative factors of Cataracts go to: https://www.drsamberne.com Have questions? Join me on Facebook Live each week at 5pm PST. https://facebook.com/samberneod Disclaimer: The information presented is intended for educational purposes only. The information presented is in no way intended as a substitute for receiving professional medical care.

Cataract treatment doesn't have to cost much. There are options that don't involve surgery. Find out what they are and how it can help you or your loved one with cataracts.

This is a critical relationship to understand if you want to dissolve cataracts naturally.

Cataracts are a common eye condition that typically develops after the age of 55. Learn what cataracts are, symptoms, causes and solutions.

Considerable amounts of some chemicals, such as sodium citrate, are allowed to add to the weight of the meat. The use of ozone and hydrogen peroxide to deodorize meat causes instantaneous oxidative changes, including lipid peroxidation and protein carbonyl formation, as well as increasing water retention.

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Dipyridamole is a drug in use for the past 55 years for treating heart conditions. In 2012 a breakthrough was reached when Moshe Rogosnitzky discovered that dipyridamole eye drops are effective in treating difficult eye conditions such as dry eye, pterygium, pinguecula, cataract, nevus and a variety of other eye disorders.