2016

Introduction Aloe Vera is a plant that belongs to the Aasphodelacea (Liliaceae) family has been used as an herbal medicine for centuries. It is native to Africa and many Mediterranean countries and is used for several healing purposes, like treating various skin disorders such as abcesses, burns and eczema. Its juice

ABSTRACT Objective: Micropropagation and elicitation studies of Aloe vera Methods: Standardisation of the regeneration protocol was done by using Murashige and Skoog (MS) media in combination with various plant growth regulators and elicitation studies were carried out by means of abiotic elicitors (salicylic acid, fructose and sodium chloride). Results: Direct regeneration through shoot tip gave the best response (0.2 mg/l Indole Butyric Acid (IBA) + 0.2mg/l 6-Benzylaminopurine (BAP)). Salicylic acid proved to be an effective abiotic elicitor, for suspension cultures of in vitro grown callus and also in vivo. It increased the content of polysaccharides, up to 7 times and phenolic compounds to almost 10 folds compared to untreated control kept under similar conditions. Conclusion: This study will make a path for the genetic transformation and metabolite enhancement along with their regulation, which can prove to be an asset towards pharmaceutical industry. Keywords: Aloe vera, Succulent plant, Micropropagation, Elicitation, Callus, Explant.

The human colonic bacterium Bacteroides thetaiotaomicron, which plays an important role in maintaining human health, produces an extensive array of exo-acting glycoside hydrolases (GH), including 32 family GH2 glycoside hydrolases. Although it is likely that these enzymes enable the organism to utilize dietary and host glycans as major nutrient sources, the biochemical properties of these GH2 glycoside hydrolases are currently unclear. Here we report the biochemical properties and crystal structure of the GH2 B.

Aloe vera healed wounds earlier than untreated rats with gradual improvement in wound areas and collagen content. Aloe vera also improved the expression of IGF-1 and VEGF in skin and bone marrow indicating improvement in angiogenesis. RT- PCR analysis showed increased expression of genes for chemota …

The International Scientific Research Organization for Science, Engineering and Technology (ISROSET) is a Non-Profit Organization; The ISROSET is dedicated to improvement in academic sectors of Science (Chemistry, Bio-chemistry, Zoology, Botany, Biotechnology, Pharmaceutical Science, Bioscience, Bioinformatics, Biometrics, Biostatistics, Microbiology, Environmental Management, Medical Science, Forensic Science, Home Science, Library Science, Material Science Military Science, Physical Science, Physical Education Science, Educational Science, Fisheries, seed technology, Agriculture, Forestry Science, Mathematics, Physics, Statistics and Geology/Earth Science), Computer Science, Engineering and Information Technology, Commerce, Management, Economics Sociology and Social Science.

Objective: Starvation have pro-oxidative effect as a consequence of both elevated ROS generation and defeat in neutralization of ROS. Aloe vera juice induced oxidative stress and significantly decreased antioxidant enzymes levels. The main aim of this experiment was to evaluate the effect of Aloe vera gel extract on oxidative stress status during long period starvationMethod: Twenty-four mature male albino Wistar rats were housed in standard cages. In this study starvation cycle (rats were starved for two days and then were fed for one day) was used. This study was performed during intermediate period (60 days).Animals were divided into four experimental groups (six rats in each group): 1) normal control; 2) starved rats + water/ethanol; 3) starved rats + hydro-alcoholic Aloe vera gel extract (100 mg/kg); 4) starved rats + hydro-alcoholic Aloe vera gel extract (200 mg/kg); 5) Without starvation with hydro-alcoholic Aloe Vera gel extract (100 mg/kg); 6) Without starvation with hydro-alcoholic Aloe Vera gel extract (200 mg/kg). Blood samples were obtained using cardiac puncture. In blood samples, antioxidant enzymes including SOD, GPx and catalase, antioxidant trace elements including copper, zinc and magnesium and antioxidant vitamins including vitamin A, vitamin E and vitamin C were measured Results: Plasma levels of antioxidant enzymes (SOD,GPX and catalase) significantly decreased in starved rats + water/ethanol group (P< 0.05). Plasma levels of antioxidant enzymes after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg were significantly increased (P < 0.05). Also, plasma levels of antioxidant enzymes in normal rats (without starvation) after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg were significantly increased (P < 0.05). Plasma levels of Cu, Zn and Mn in normal control group had no significant difference with starved rats + water/ethanol and starved rats + hydro-alcoholic Aloe vera gel extract in 100 and 200 mg/kg dose groups (P > 0.05). Plasma levels of vitamin A and E in normal control group had no significant difference with starved rats + water/ethanol and starved rats + hydro-alcoholic Aloe vera gel extract in the 100 and 200 mg/kg dose groups (P > 0.05). Plasma level of vitamin C significantly decreased in starved rats + water/ethanol group (P < 0.05). Plasma level of vitamin C after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg was significantly increased (P < 0.05). Also, plasma level of vitamin C in normal rats (without starvation)  after treatment with hydro-alcoholic Aloe vera gel extract at doses 100 and 200 mg/kg was significantly increased (P < 0.05)Conclusion:In concluding our results it is shown that intermediate period starvation caused  an increase in oxidative stress via impairing of antioxidant defense and Aloe vera treatment is able  to improve antioxidative defense induced by starvation

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Aloe vera (Aloe barbadensis Miller) is a perennial succulent medicinal plant. It has been used as a traditional or folk medicine for thousands of year…

The co-administration of absorption enhancing agents with macromolecular drugs (e.g., protein and peptide drugs) has been identified as a means to improve the oral bioavailability of these drugs. Absorption-enhancing agents of natural origins have received ...

Study Design: This is an experimental study using an animal model. Objectives: Disk degeneration is a common cause of low back pain. However, few attempts have ...

Food industries typically use Aloe vera as concentrated (100&#xd7; to 200&#xd7;) and dried powders in their final products. These powders are obtained by extrusion of Aloe inner leaf gel (ILG) or Aloe whole leaf (WLP); the juice is filtered through diatomaceous earth and activated carbon before spray drying at temperatures below 70 &#xb0;C. In another process, Aloe inner leaf gel was dried at &#x7e;80 &#xb0;C and mashed to a powder rich in high molecular weight fibres and soluble polysaccharides (ILF). In contrast to ILG and WLP, the ILF sample was cytotoxic for the human intestinal cell line Caco-2 (CC50 = 1&#x2009;g/l), even at concentrations below the recommended dose for human consumption. At lower concentrations (250&#x2009;mg/l) with LPS challenged macrophage-like THP-1 cells decreased by 40&#x25; the release of the anti-inflammatory cytokine IL-10, whereas the release of the proinflammatory cytokine IL-1&#x3b2; increased by 35&#x25; (compared to untreated but challenged macrophage-like THP-1 cells). Unexpectedly, under the same conditions, the less cytotoxic ILG and WLP, both samples with a lower fibre content, significantly increased (up to 2.4 times) the release of IL-10, while the concentration of IL-1&#x3b2; remained unaltered and of TNF&#x3b1; decreased by 35&#x25;. Even more interesting is that a treatment of the ILF sample with activated carbon reduced its cytotoxicity and increased the IL-10 release (3.1 times). Based on these results, we suggest applying an activated carbon treatment on Aloe-starting products, which have high fibre content and have received high temperature treatment, in order to reduce their cytotoxicity and improve their immunomodulatory properties.

Aloe vera leaf contains some bioactive compounds that have a strong binding affinity toward estrogen receptor as compared to standard drug tamoxifen. In this study, we have found that the IC50 of Aloe vera leaf extract against breast cancer cell line (MCF-7) is 23 µg/mL whic …

Background. Sickle cell disease (SCD) is an inherited haemolytic anemia with a variable course and severity. Knowledge of prognostic biomarkers may help in the establishment of therapeutic intervention, management, and follow-up of patients. There have been scattered reports of low high-density lipoprotein cholesterol (HDL-C) and increased triglyceride (TG) in SCD patients. In addition, TG levels have been suggested to be elevated in patients with increased endothelial activation. An increased TG level has been associated with haemolysis, vascular dysfunction, and increased prevalence of pulmonary hypertension. Gum Arabic (GA) is an edible, dried, gummy exudate from the acacia Senegal tree. Several studies on GA ingestion have shown reduced plasma cholesterol and low-density lipoprotein (LDL) concentrations in both animals and humans. We investigated GA&#x2019;s therapeutic potential to modulate serum lipids in patients with sickle cell anemia. Methods. This study recruited and documented secondary outcomes in 47 patients (aged 5&#x2013;42 years) carrying hemoglobin SS. The patients received 30 g/day of GA for 12 weeks. Total cholesterol, TG, LDL, and HDL were measured before and after GA intake. Cobas C311 (Roche, Germany) automated chemistry analyser was used for direct determination of the values of the lipid profile. Results. GA significantly decreased total cholesterol (TC), TG, and LDL (p = 0.006, 0.04, and 0.02, resp.). GA showed no effect on HDL level. Baseline serum TG and LDL correlated significantly with the hydrogen peroxide (H2O2) level, which is known as an oxidative stress marker (p = 0.003 and 0.04, resp.). None of the lipid profile elements correlated with age. Conclusion. Our results revealed that dyslipidemia in sickle cell patients is associated with oxidative stress but not associated with age. The findings showed that GA significantly decreased TC, LDL, and TG levels, revealing a novel effect of GA, which is considered a natural dietary fibre that can modulate lipid profile in patients with sickle cell anemia. Trial Registration. This retrospective trial is registered with ClinicalTrials.gov Identifier: NCT02467257 on 3 June, 2015.

SARS-CoV-2 is the pathogen agent of the new corona virus disease that appeared at the end of 2019 in China. There is, currently, no effective treatmen…

Treatment of many inflammatory diseases involves a chronic use of NSAIDs in large doses increasing acute kidney injury risk. This study was designed t…

Based on activation of macrophages and vaccine adjuvant activity by aloe polysaccharide or acemannan, we showed that the importance of butyrate and host-micRNA-microbiota interaction in regulation of gut immunity, and butyrate is a chemical factor capable of facilitating induced pluripotent stem cell generation, an important step for the establishment of pluripotency. Furthermore we exhibited three case reports on the beneficial roles of aloe vera juice-successive ingestion as nutritional and medicinal uses to influenza virus and norovirus.

Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and…

The aim of the present study was to explore the adjunctive use of Acacia arabica gel in the treatment of chronic periodontitis.Single centre, randomised, triple blind, controlled trial on mild to moderate chronic periodontitis patients; Group I (SRP + Acacia ...

Aloe vera (L.) Burm. f. (Aloe barbadensis Mill) Liliaceae, succulent plant native to northern Africa, is presently cultivated in many regions of the world. Traditionally, its inner part of parenchyma, which contains aloe gel, was used for the treatment ...

Acacia gum is a branched complex polysaccharide whose main chain consists of 1,3-linked β-D-galactopyranosyl units. Acacia gum is defined as a heteropolysaccharide since it contains ∼2% of a polypeptide. The major molecular fraction (F1) ...

Pre-diabetes is a disturbing trend in the population, who are at risk of developing type-two diabetes. The aim of this study was to determine the effects use of Aloe vera in different doses on glucose and lipid profile in pre-diabetic subjects.This study ...