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Hempseed Water-Soluble Protein Fraction and Its Hydrolysate Display Different Biological Features
Hempseed Water-Soluble Protein Fraction and Its Hydrolysate Display Different Biological Features
Hempseeds, from the Cannabis sativa plant, and its derivates are a versatile food option for various dietary preferences. Due to their aminoacidic profile, researchers have studied the presence of bioactive peptides in hempseed proteins. In this study, the water-soluble fraction of hempseed protein was extracted, and the derived peptides were analyzed. The investigation focused on their biological function, particularly their antioxidant activity. Several biological functions have arisen, such as angiotensin-converting enzyme inhibition activity, dipeptidyl-peptidase IV, dipeptidyl-peptidase III inhibition, and ubiquitin-mediated proteolysis activation. The hydrolysates show greater 2,2-azinobis-[3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity compared to the proteins (97.95 ± 4.48 versus 81.04 ± 10.63). Furthermore, the impact of these proteins and peptides on the U937 cell line was evaluated to assess cell viability and their potential role in modulating inflammation associated with gastrointestinal autoimmune diseases. Protein treatment resulted in a significant reduction in cell viability, as opposed to hydrolysates, which did not affect it.
·mdpi.com·
Hempseed Water-Soluble Protein Fraction and Its Hydrolysate Display Different Biological Features
Hemp - Innvista
Hemp - Innvista
Pages Abstracts Articles Bibliography Common Hemp Crop Pests Growing Hemp Harvesting Hemp Hemp Around the World Hemp Biology Hemp Food Storage Hemp Oil Hemp Products Information Hemp Seed Protein Hemp Seeds Hemp Seeds as Medicine Hemp Species History of Hemp Industrial Hemp Paper Schlichten Papers
·web.archive.org·
Hemp - Innvista
Solutions for Safe, Quality Cannabis | Medicinal Genomics
Solutions for Safe, Quality Cannabis | Medicinal Genomics
Medicinal Genomics is the world leader in cannabis genetics and microbial detection. We are offering both DNA sequencing and metagenomics for cannabis products.
·medicinalgenomics.com·
Solutions for Safe, Quality Cannabis | Medicinal Genomics
! Dosage Programs - Healer
! Dosage Programs - Healer
Free step-by step cannabis dosage programs with instructions and videos to help you use cannabis to achieve the best therapeutic results.
·healer.com·
! Dosage Programs - Healer
Antioxidants | Free Full-Text | Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates
Antioxidants | Free Full-Text | Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates
Hempseed (Cannabis sativa) is one of the most promising sources of plant proteins. It contains approximately 24% (w/w) protein, and edestin accounts for approximately 60–80% (w/w) of its total proteins. In a framework of research aimed at fostering the proteins recovered from the press cake by-products generated after the extraction of hempseed oil, two hempseed protein hydrolysates (HH1 and HH2) were produced at an industrial level using a mixture of different enzymes from Aspergillus niger, Aspergillus oryzae, and Bacillus licheniformis for different times (5 h and 18 h). Using a combination of different direct antioxidant tests (DPPH, TEAC, FRAP, and ORAC assays, respectively), it has been demonstrated that HHs exert potent, direct antioxidant activity. A crucial feature of bioactive peptides is their intestinal bioavailability; for this reason, in order to solve this peculiar issue, the ability of HH peptides to be transported by differentiated human intestinal Caco-2 cells has been evaluated. Notably, by using mass spectrometry analysis (HPLC Chip ESI-MS/MS), the stable peptides transported by intestinal cells have been identified, and dedicated experiments confirmed that the trans-epithelial transported HH peptide mixtures retain their antioxidant activity, suggesting that these hempseed hydrolysates may be considered sustainable antioxidant ingredients to be exploited for further application, i.e., nutraceutical and/or food industries.
·mdpi.com·
Antioxidants | Free Full-Text | Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates
Molecules Free Full-Text In Vitro Digestibility, Biological Activity, and Physicochemical Characterization of Proteins Extracted from Conventionally and Organically Cultivated Hempseed
Molecules Free Full-Text In Vitro Digestibility, Biological Activity, and Physicochemical Characterization of Proteins Extracted from Conventionally and Organically Cultivated Hempseed
The proteins from two conventionally (CC1 and CC2) and one organically cultivated (OC) hempseed samples were extracted (by alkaline solubilization followed by isoelectric precipitation) and compared in terms of their physicochemical, digestibility and in vitro bioactivity properties. The OC hempseed had higher total protein and lower nonprotein nitrogen content. Protein extracts showed bimodal particle size distributions, with OC showing the smallest and CC1 the largest mean particle diameter (d(0.5)), i.e., 89.0 and 120.0 µm, respectively. Chromatographic analysis showed similar protein profiles for all three protein extracts. The protein extracts were subjected to in vitro simulated gastrointestinal digestion (SGID). Degree of hydrolysis (DH) measurement showed that the highest extent of digestion upon SGID was associated with CC1 (11.0 ± 1.5%), which also had the lowest in vitro antioxidant activity. Only the OC and OC digested samples had lipase inhibitory activity. The results indicate that the cultivation method impacted the composition, physicochemical, digestibility, and biofunctional properties of hempseed proteins.
·mdpi.com·
Molecules Free Full-Text In Vitro Digestibility, Biological Activity, and Physicochemical Characterization of Proteins Extracted from Conventionally and Organically Cultivated Hempseed
Bulk Hemp Warehouse Home Is Where The Hemp Is
Bulk Hemp Warehouse Home Is Where The Hemp Is
Get your Bulk Hemp Seed and Hemp Fiber Products to make your projects and products a success! Choose from over 300 products and hempower your company and life.
·bulkhempwarehouse.com·
Bulk Hemp Warehouse Home Is Where The Hemp Is
RSHO Green Label CBD Products from HempMeds
RSHO Green Label CBD Products from HempMeds
RSHO Green Label is closest to the organic makeup of the natural hemp plant, as it is kept in its raw form, containing vitamins, minerals, fatty acids...
·hempmedspx.com·
RSHO Green Label CBD Products from HempMeds
Hempseed A Functional Food Source
Hempseed A Functional Food Source
MOLECULAR MECHANISMS OF FUNCTIONAL FOOD Comprehensive resource for understanding state-of-the-art mechanisms behind food health effects This book provides information on the development and validation of functional foods towards their market and industrial application. It covers the available information on developments, efficacy, and testing and safety, while demonstrating the proven or potential effects of food on health and disease. With contributions from the foremost experts in the field, this book will bring readers up to speed on the state of the art in the mechanisms behind food health effects, from their physiological bases to their conception, current uses, and future developments. Sample topics covered by the authors include: The molecular mechanisms of action of antioxidant fibers, prebiotics, ginger, and cinnamon Saffron, a functional food with potential molecular effects Pseudocereals, ancestral grains that can serve as a source of bioactive compounds for functional foods Molecular effects of future functional foods – psychobiotics, chronobiotics, hempseed, opuntia, common and carioca beans, coffee and cocoa by-products Food industry professionals, government workers involved in projects related to food, and students in programs of study related to food can use this book as an up-to-date reference to fully understand the effects that many common and uncommon foods have on humans.
·books.google.com·
Hempseed A Functional Food Source
FullBroad Spectrum vs. Isolate - Ananda Professional
FullBroad Spectrum vs. Isolate - Ananda Professional
CBD Isolate, full spectrum, and broad spectrum. Which type of CBD is best for you? Read our in depth discussion so you can make a well-informed decision.
·anandaprofessional.com·
FullBroad Spectrum vs. Isolate - Ananda Professional
Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications Scientific Reports
Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications Scientific Reports
Emerging formulation technologies aimed to produce nanoemulsions with improved characteristics, such as stability are attractive endeavors; however, comparisons between competing technologies are lacking. In this study, two formulation techniques that employed ultrasound and microfluidic approaches, respectively, were examined for relative capacity to produce serviceable oil in water nanoemulsions, based on hempseed oil (HSO). The ultrasound method reached > 99.5% entrapment efficiency with nanoemulsions that had an average droplet size (Z-Ave)
·nature.com·
Comparing microfluidics and ultrasonication as formulation methods for developing hempseed oil nanoemulsions for oral delivery applications Scientific Reports