C Difficile

A study presented at the Society for Healthcare Epidemiology of America (SHEA) conference assesses the link between antibiotic use and C difficile infection in Tennessee.

Allowing bedside nurses the autonomy to order testing for clostridium difficile results in faster turnaround times, researchers report.

The long-term health care burden after Clostridium difficile (C diff) infection (CDI) is great for elderly patients. But even nonelderly patients are at risk for gastrointestinal symptoms for up to 2 years afterward.

Although no longer indicated as a first-line therapy, metronidazole still has therapeutic benefits and can serve patients in certain settings and circumstances.

Laura Gillespie, PharmD, discusses the impact of pharmacist-led initiatives on health care–associated C diff rates for health care systems.

Download scientific diagram | E-cadherin of IECs interacts with increasing C. difficile spores in TcdA- and TcdB-intoxicated cells. Differentiated Caco-2 cells were intoxicated with TcdA and TcdB for 8 h at 37°C in DMEM without serum. As a control, cells were incubated with DMEM without serum. Then, cells were infected with C. difficile spores for 1 h at 37°C. Nonpermeabilized cells were stained for accessible E-cadherin (shown as acc Ecad; green), spores (red), and nuclei (blue). (A) Representative 3D projection confocal micrograph of healthy cells infected with C. difficile spores (Ctrl). (A, Right) Magnifications of representative C. difficile spores associated or not with E-cadherin. (B and C) Plot profiles of fluorescence intensity of C. difficile spores (red line) and accessible E-cadherin (shown as Ecad, green line) performed in the white line of panel A. (D) Representative 3D projection confocal micrograph of TcdA- and TcdB-intoxicated cells infected with C. difficile spores. (D, Right) Magnifications of representative C. difficile spores associated or not with E-cadherin. (E and F) Plot profiles of fluorescence intensity of C. difficile spores (red) and accessible E-cadherin (shown as Ecad, in green) performed in the white line of panel D. Repartition of spores that were positive (Ecad+) or negative (Ecad−) associated with fluorescence signal for accessible E-cadherin was shown as the average associated/nonassociated spores with E-cadherin for each field; a total of ~500 spores were analyzed. Fluorophores were digitally reassigned for a better representation. Micrographs are representative of 6 independent fields of 3 different experiments. Error bars indicate mean ± SEM. Statistical analysis was performed by two-tailed unpaired Student’s t test. P ≤ 0.01; ***, P ≤ 0.001. Bars, 10 μm. from publication: Redistribution of the Novel Clostridioides difficile Spore Adherence Receptor E-Cadherin by TcdA and TcdB Increases Spore Binding to Adherens Junctions | Clostridioides difficile causes antibiotic-associated diseases in humans, ranging from mild diarrhea to severe pseudomembranous colitis and death. A major clinical challenge is the prevention of disease recurrence, which affects nearly ~20 to 30% of the patients with a... | Spores, Cadherins and Adherens Junctions | ResearchGate, the professional network for scientists.

Obesity is becoming a major global health problem in children that can cause diseases such as type 2 diabetes and metabolic disorders, which are closely related to the gut microbiota. However, the underlying mechanism remains unclear. In this study, a significant positive correlation was observed be …

Our approach provides new opportunities to rationally harness live gut probiotic strains and metabolites to reduce intestinal inflammation, restore gut microbial composition, and expedite the development of safe and effective treatments for IBD.

Beneficial gut bacteria are indispensable for developing colonic mucus and fully establishing its protective function against intestinal microorganisms. Low-fiber diet consumption alters the gut bacterial configuration and disturbs this microbe-mucus interaction, but the specific bacteria and microb …

WMT demonstrated therapeutic efficacy in CD treatment, likely due to the effective reconstruction of the patient's microbiome. Multi-omics techniques can effectively help decipher the potential mechanisms of WMT in treating CD.

The role of the microbiome in immunotherapy has recently garnered substantial attention, with molecular studies and clinical trials providing emerging evidence on the pivotal influence of the microbiota in enhancing therapeutic outcomes via immune response modulation. However, the impact of microbia …

The gut microbiota is recognized as an endocrine organ with the capacity to influence distant organs and associated biological pathways. Recent advancements underscore the critical role of gut microbial homeostasis in female health; with dysbiosis potentially leading to diseases among women such as …

Chronic constipation is one of the common gastrointestinal disorders, with an incidence rate that is gradually increasing yearly and becoming an important chronic disease that affects people's health and quality of life. In recent years, significant progress has been made in the basic and clinical r …

The gut microbiota is a crucial component of the intricate microecosystem within the human body that engages in interactions with the host and influences various physiological processes and pathological conditions. In recent years, the association between dysbiosis of the gut microbiota and tumorige …

Dynamic changes in gut dysbiosis and metabolomic dysregulation are associated with immune-complex glomerulonephritis (ICGN). However, an in-depth study on this topic is currently lacking. Herein, we report an ICGN model to address this gap. ICGN was induced via the intravenous injection of cationize …

Non-alcoholic fatty liver disease (NAFLD) is a type of metabolic stress liver injury closely related to insulin resistance (IR) and genetic susceptibility without alcohol consumption, which encompasses a spectrum of liver disorders ranging from simple hepatic lipid accumulation, known as steatosis, …

Despite the technologies applied to food production, microbial contamination and chemical deterioration are still matters of great concern. In order to limit these phenomena, new natural approaches should be applied. In this context, the present study aimed to assess the antioxidant and anti-Clostri …

In our cohort, the duration of hospital stay seemed to be shorter in the very elderly with no increase of in-hospital and post-discharge mortality. Although admitted less frequently to ICU, the in-hospital survival of the very elderly was not adversely affected compared to the elderly, suggesting th …

Clostridioides difficile (C. difficile) is the most common agent of antibiotic-associated diarrhea, leading to intestinal infection through the secretion of two major toxins. Not all strains of this bacterium are toxigenic, but some of them cause infection via their accessory virulence factors, such …

To overcome the deficiencies of conventional methods, the potential of advanced methods for C. difficile diagnosis, their direction, perspective, and challenges ahead were discussed.

Antibiotics and proton pump inhibitors may not be the best mix, according to a study published Jan. 24 in the Journal of Antimicrobial Chemotherapy.

Incidence of hospital-onset Clostridioides difficile infection differed between hospitals that implemented the CDC prevention strategies and those that did not, but not necessarily because of the strategies.

Download scientific diagram | Changes in Clostridioides difficile tests and CDI cases from January 2015 to March 2018, divided into quarters, with the dotted line indicating 2016 quarter 1 as the intervention period. (A) C. difficile test volumes for inpatients. (B) Reported HO-CDI rates. (C) Distribution of diarrheal episodes among LabID HO-CDI events. (D) Laxative use among LabID HO-CDI events. (E) Laxative use among LabID HO-CDI events with clinically significant diarrhea, defined as ≥3 unformed stools within 24 h prior to testing. (F) Numbers of reported HO-CDI events and true HO-CDI events, after removal of events with laxative use and/or insignificant diarrhea (

Research coming in from the University of Texas Health Science Center at Houston shows that when it comes to treating those with C. difficile, frozen and freeze-dried products for Fecal Microbiota Transplantation are nearly as effective as fresh product.

A new review recommends updated guidelines for diagnosing and managing Candida and C diff coinfection.

U.S. National Institute of Diabetes and Digestive and Kidney Diseases.

Up to 25% of patients recovering from antibiotic-treated Clostridioides difficile infection (CDI) develop functional symptoms reminiscent of Post-Infectious Irritable Bowel Syndrome (PI-IBS). For patients with persistent symptoms following infection, a clinical dilemma arises as to whether to provid …

The gut microbiota modulates response to hormonal treatments in prostate cancer (PCa) patients, but whether it influences PCa progression remains unknown. Here, we show a reduction in fecal microbiota alpha-diversity correlating with increase tumour burden in two distinct groups of hormonotherapy na …

The key to understanding and effectively treating Clostridioides difficile infection is noting its two phases: the vegetative state that releases toxins and causes ...