0 Glyconutrients

Bladder cancer (BC) is a common urological cancer, with poor prognosis for advanced/metastatic stages. Various intensive treatments, including radical cystectomy, chemotherapy, immune therapy, and radiotherapy are commonly used for these patients. However, these treatments often cause complications …

Two field experiments were conducted during two successive seasons (2014/2015 and 2015/2016) at the Experimental Station of National Research Centre, Nubaria district, Beheira Governorate, Egypt, to study the effect of foliar treatment of quinoa plants with trehalose (Tre) (100µM and 500µM) on growth, photosynthetic pigments, seed yield quantity & quality, in fever of nutritional and antioxidant compounds in the yielded quinoa seeds which subjected to water deficiency (skipping two irrigation times at 50 & 60 days after sowing). Water deficiency caused marked decreases in quinoa plant growth parameters (shoot height, fresh and dry weights of shoot/plant) and photosynthetic pigments with marked increases in root growth parameters (root length, fresh and dry weight of root/plant). Drought stress decreased yield and yield attributes, carbohydrates, protein, nitrogen, phosphorous and potassium contents.  Meanwhile oil percentage, phenolic and flavonoids contents increased by drought stress. Antioxidant activity at 50 and 100µg/l showed significant increases in response to drought stress. On the other hand, Tre treatments proved to be effective in enhancing growth parameters and photosynthetic pigments of drought stressed plants. Trehalose treatments at different levels caused marked increases in yield and yield attributes, carbohydrate, protein, oil, nitrogen, phosphorous, potassium, total phenolic, flavonoids contents, and antioxidant activity of the yielded seeds either in non stressed or drought stressed plants relative to corresponding controls. Generally, 500 µM Tre was the most pronounced and effective treatment in alleviating the deleterious effect of drought stress on quinoa plants.

There are many plants with interesting pharmaceutical activities but Aloe vera is probably the most applied medicinal plant worldwide. Since biblical …

Fucoidan is a sulfated polysaccharide derived from brown algae and possesses various beneficial activities, including antioxidant property. Previous s…

Food industries typically use Aloe vera as concentrated (100× to 200×) and dried powders in their final products. These powders are obtained by extrusion of Aloe inner leaf gel (ILG) or Aloe whole leaf (WLP); the juice is filtered through diatomaceous earth and activated carbon before spray drying at temperatures below 70 °C. In another process, Aloe inner leaf gel was dried at ~80 °C and mashed to a powder rich in high molecular weight fibres and soluble polysaccharides (ILF). In contrast to ILG and WLP, the ILF sample was cytotoxic for the human intestinal cell line Caco-2 (CC50 = 1 g/l), even at concentrations below the recommended dose for human consumption. At lower concentrations (250 mg/l) with LPS challenged macrophage-like THP-1 cells decreased by 40% the release of the anti-inflammatory cytokine IL-10, whereas the release of the proinflammatory cytokine IL-1β increased by 35% (compared to untreated but challenged macrophage-like THP-1 cells). Unexpectedly, under the same conditions, the less cytotoxic ILG and WLP, both samples with a lower fibre content, significantly increased (up to 2.4 times) the release of IL-10, while the concentration of IL-1β remained unaltered and of TNFα decreased by 35%. Even more interesting is that a treatment of the ILF sample with activated carbon reduced its cytotoxicity and increased the IL-10 release (3.1 times). Based on these results, we suggest applying an activated carbon treatment on Aloe-starting products, which have high fibre content and have received high temperature treatment, in order to reduce their cytotoxicity and improve their immunomodulatory properties.

The present work aims to investigate the effects and underlying mechanism of a homogeneous Laminaria japonica polysaccharide (LJP61A) on acute kidney …

Objective: To assess the safety and efficacy of weekly IV administration of Cabaletta (trehalose 9\[percnt] solution) in OPMD after a 24 week open label phase 2 trial. Background: Trehalose showed efficacy in reducing PABPN1 muscle aggregation and improving muscle function in a transgenic OPMD mouse model. Methods:25 genetically-confirmed OPMD patients received weekly infusion of 300 cc Cabaletta. Swallowing, muscle power and functional tests, and swallowing quality of life report (SWAL-QOL) were assessed at baseline and after 24 weeks. Results: No serious drug-related adverse effects were noted. Time to swallow 80cc cold water (an OPMD validated dysphagia test) improved by 35.3[percnt\] (p

A wound dressing is very crucial component in wound healing process. Bioactive dressings play important role in wound sterilization and promote tissue healing and growth. The present work investigated the preparation of AgNPs in solid-state using acacia gum as both reductant and stabilizing agent. Sodium hydroxide (NaOH) was employed as activating agent and pH mediator. Acacia is a naturally occurring mixture of polysaccharides and glycoproteins. The obtained particles were in the range of 50 nm. The work was extended to evaluate the antimicrobial of AgNPs treated gauze cotton fabrics against gram positive (S. aureus), gram negative (P. aeruginosa) bacteria, C. albicans (yeast); and A. Niger (fungus). The inhibition zone of the as prepared silver nanoparticles was found to be 24 mm against both types of bacteria, 23 mm against C. albicans (yeast), and inactive against A. Niger (fungus). On the other hand, the treated gauze showed bactericidal behavior and a clear zone was found underneath the samples on the agar plate. The reduction percent in number of bacterial colonies of treated gauze fabrics in comparison to control Pseudomonas aeruginosa culture showed a reduction up to 100%. The aforementioned results promote the acquirement of bioactive antibacterial wound dressing.

Increased added sugar is contributing to a rise in aging-related diseases. Here, we use the nematode, Caenorhabditis elegans , which store sugar as both glycogen and trehalose. We demonstrate that by modifying sugar storage, we can prevent the harmful effects of a high-sugar diet. Our data show that a metabolic shift increasing the glucose disaccharide trehalose, and decreasing the glucose polysaccharide glycogen, extends lifespan and promotes healthy aging. The positive effects of trehalose require the DAF-16 transcription factor and the process of autophagy. Our data reveal the benefits of trehalose for prolonged health in the face of our high-sugar environment.

Protective effect of fucoidan from Fucus vesiculosus on liver fibrosis via the TGF-β1/Smad pathway-mediated inhibition of extracellular matrix and autophagy Jingjing Li,1 Kan Chen,1 Sainan Li,1 Jiao Feng,1 Tong Liu,1 Fan Wang,1 Rong Zhang,1,2 Shizan Xu,1,2 Yuqing Zhou,1,3 Shunfeng Zhou,1,3 Yujing Xia,1 Jie Lu,1 Yingqun Zhou,1 Chuanyong Guo1 1Department of Gastroenterology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 2The First Clinical Medical College of Nanjing Medical University, Nanjing, 3Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, People’s Republic of China Abstract: Liver fibrosis is a dynamic reversible pathological process in the development of chronic liver disease to cirrhosis. However, the current treatments are not administered for a long term due to their various side effects. Autophagy is initiated to decompose damaged or excess organelles, which had been found to alter the progression of liver fibrosis. In this article, we hypothesized that fucoidan from Fucus vesiculosus may attenuate liver fibrosis in mice by inhibition of the extracellular matrix and autophagy in carbon tetrachloride- and bile duct ligation-induced animal models of liver fibrosis. The results were determined using enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemical staining. Fucoidan from F. vesiculosus could inhibit the activation of hepatic stellate cells and the formation of extracellular matrix and autophagosomes, and its effect may be associated with the downregulation of transforming growth factor beta 1/Smads pathways. Fucoidan, as an autophagy and transforming growth factor beta 1 inhibitor, could be a promising potential therapeutic agent for liver fibrosis. Keywords: liver cirrhosis, hepatic stellate cells, bile duct ligation

What is Aloe Vera? Aloe Vera is a cactus-like plant which grows in the tropics. The stems are short, growing to a maximum height of 3 1/2 feet. The leaves are thick, green and fleshy, containing a gel-like substance, which is used for several medicinal purposes. It actually stores water in the leaves. The leaves are Read More

Fucoidan is a unique bioactive and dietary polymer enriched mainly in the cell wall matrix of the brown seaweeds. This present study was intended to reveal the antigenotoxicity effect of fucoidan on 4-nitroquinolin-1-oxide (4-NQO) induced genetics damage and apoptosis in mice bone marrow cells. The 4-NQO caused genetic damages in the form of chromosome/chromatic breakage was estimated by micronuclei assay whereas apoptosis by annexin-V FITC kit and DNA damage by comet assay kit. In addition, oxidative damage in terms of plasma lipid peroxidation (LPO) and 8-OHdG was also estimated. In the experimental regime, six groups with each in five either sex of mice were used. Fucoidan constituted (50,100,200 mg/kg bwt) by orally for 5 days consequently and on 6th day, 4-NQO was administered (7.5 mg/kg bwt) by i.p. The results clearly show that negative control (H2O) and fucoidan alone constituted mice were not exhibited significant effect on LPO, genetic damages whereas positive control group (4-NQO 7.5 mg/kg bwt, i.p.) showed significant effect on genetic damage by showing increased level of LPO (6.25 vs 1.3 μM MDA), 8-OHdG (12 vs 4%), micronuclei about six-fold, 5-fold of comet, and 4-fold of apoptosis when compared with negative control, 11.6 ± 2.07, 5.00 ± 1.58, and 4.14 ± 0.65 respectively. Fucoidan pretreatment significantly protected the 4-NQO-induced genetic damage by 77% decreased level of micronuclei and 96% comet at dose of 200 mg/kg bwt over the positive control whereas LPO, 8-OHdG, and apoptosis were restored as equal to negative control. This study found as fucoidan possessing significant antigenotoxicity property by protecting 4-NQO-induced genetic damage in mice bone marrow cells as dose dependent manner suggest as valuable food supplements and medicine for mankind from environmental toxicants.

Diabetes mellitus is the most prevalent metabolic disorder contributing to significant morbidity and mortality in humans. Many preventative and therapeutic agents have been developed for normalizing glycemic profile in patients with diabetes. In addition to various pharmacologic strategies, many non …

Trehalose is a natural disaccharide that is found in a diverse range of organisms but not in mammals. Autophagy is a process which mediates the sequestration, lysosomal delivery and degradation of proteins and organelles. Studies have shown that trehalose exerts beneficial effects through inducing a …

Zinc (Zn) deficiency is a clinical consequence of brain injury that can result in neuropathological outcomes that are exacerbated with age. Here, we present laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) imaging data showing modulation of brain Zn levels by the disaccharide trehalose

High oxygen mechanical ventilation is widely used to treat various lung diseases; however, it may result in hyperoxia, which induces inflammation and lung injury. Fucoidan is an extract of the seaweed Fucus vesiculosus, which has previously been reported to exert effects against diabetic nephropathy …

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Purpose Phase separation of trehalose during freeze-drying could render it ineffective as a lyoprotectant. The bulking agent, mannitol, on the other hand, should crystallize readily upon freezing. It is therefore imperative to understand the mutual interaction of these sugars during freezing to ensure preservation of the API during freeze-drying. Methods We investigated the effect of mannitol to trehalose ratio (R) on the crystallization behavior of both solutes using Differential Scanning Calorimetry, X-Ray Crystallography and FTIR Spectrosopy during controlled freezing and annealing. Results When R = 1, crystallization of both mannitol (as hemihydrate) and trehalose (as α-anhydrate) were observed. When R ≥ 1, extent of mannitol crystallization was directly proportional to the value of R. When R  1, the freeze concentrate was heterogeneous and characterized by two glass transitions – the lower temperature transition (Tg”) attributed to systems containing “extra” unfrozen water. When heated above Tg”, crystallization of mannitol and the associated unfrozen water resulted in Tg’ (glass transition temperature of the freeze-concentrate). Conclusions R and not the total solute concentration, dictates the composition of the freeze concentrate as well as the physical stability of the excipients

This study investigated the protective effect of the butanol (BuOH) fraction from Laminaria japonica (BFLJ) extract on high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Freeze-dried L japonica was extracted with distilled water, and the extracted solution was mixed with ethanol then centrifuged. The supernatant was subjected to sequential fractionation with various solvents. The BuOH fraction was used in this study because it possessed the strongest antioxidant activity among the various solvent fractions. To determine the protective effect of the BFLJ, oxidative stress was induced by exposing of HUVECs to the high glucose (30 mM) or normal glucose (5.5 mM) for 48 hr. Cell viability, lipid peroxidation, glutathione (GSH) concentration, and antioxidant enzyme activities such as catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-px), and glutathion reductase (GSH-re) were measured. Exposure of HUVECs to high glucose for 48 hr resulted in a significant (p

Study Design: This is an experimental study using an animal model. Objectives: Disk degeneration is a common cause of low back pain. However, few attempts have ...

Fucoidan has been reported to exhibit various beneficial activities ranging from to antivirus and anticancer properties. However, little information is available about the effects of fucoidan on cerebral ischemia-reperfusion injury (IRI). Our study aimed to explore the effects of fucoidan on cerebra …

Fucoidan has been reported to exhibit various beneficial activities ranging from to antivirus and anticancer properties. However, little information is available about the effects of fucoidan on cerebral ischemia-reperfusion injury (IRI). Our study aimed to explore the effects of fucoidan on cerebra …

The co-administration of absorption enhancing agents with macromolecular drugs (e.g., protein and peptide drugs) has been identified as a means to improve the oral bioavailability of these drugs. Absorption-enhancing agents of natural origins have received ...

Background/Aim: Administration of cisplatin in cancer patients is limited by the kidney-related adverse effects; however, a protective strategy is absent. We hypothesized that fucoidan protects the proximal tubule epithelial (TH-1) cells against the effects of cisplatin. Materials and Methods: To assess the effect of fucoidan, its effect on reactive oxygen species (ROS) formation, endoplasmic reticulum (ER) stress response, DNA damage response (DDR), apoptosis, and cell-cycle arrest in TH-1 cells was investigated. Results: Cisplatin increased the accumulation of ROS, leading to excessive ER stress. In presence of cisplatin, treatment of TH-1 cells with fucoidan significantly reduced the ER stress by maintaining the complex of GRP78 with PERK and IRE1α. In particular, fucoidan enhanced the antioxidative capacity through up-regulation of PrPC. Furthermore, fucoidan suppressed cisplatin-induced apoptosis and cell-cycle arrest, whereas silencing of PRNP blocked these effects of fucoidan. Conclusion: Fucoidan may be a potential adjuvant therapy for cancer patients treated with cisplatin as it preserves renal functionality.

Fucoidans are sulfated polysaccharides that are extracted from brown algae. Fucoidans with low molecular weight (LMWF) have been revealed various biological activities and health benefits. The aim of...

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Accumulations of radionuclides in marine macroalgae (seaweeds) resulting from the Fukushima 1 Nuclear Power Plant (F1NPP) accident in March 2011 have been monitored for two years using high-purity germanium detectors. Algal specimens were collected seasonally ...