0 Glyconutrients

Acacia gum is a branched complex polysaccharide whose main chain consists of 1,3-linked β-D-galactopyranosyl units. Acacia gum is defined as a heteropolysaccharide since it contains ∼2% of a polypeptide. The major molecular fraction (F1) ...

Fucoidan is a complex of polysaccharides showing antitumor and immunomodulation properties. Our previous studies found its regulation to myeloid immune cells, including macrophages. Aberrant infiltration and functions of macrophages are commonly found in oral squamous cell carcinoma (OSCC). In this study, we analyzed the effects of fucoidan on invasion of OSCC cells, and their regulation to macrophages, trying to evaluate its role as a potential therapy for OSCC. CAL27 and THP-1-derived macrophages were used as models for OSCC cells and tumor-infiltrated macrophages in the in vitro study, respectively. The effects of fucoidan on invasion of OSCC cells and their recruitment to macrophages were analyzed by transwell assay. KIF4A siRNA transfection was performed to investigate its role in fucoidan-modulated OSCC cells invasion. CCL3-neutralizing antibody was added into the conditioned medium of OSCC cells to evaluate its role in fucoidan-mediated macrophages recruitment and re-education. Fucoidan reduced the invasive potential of CAL27 cells with a decrease of MMP-2 and KIF4A transcription. KIF4A knockdown in CAL27 cells led to decreased invasion and MMP-2 expression. The conditioned medium of fucoidan-treated CAL27 cells promoted recruitment and inflammatory cytokines secretion on THP-1-derived macrophages. Further analysis found that fucoidan increased CCL3 production in CAL27 cells. Blocking CCL3 expression reversed the effects of fucoidan on macrophage recruitment and re-education. Our study found that fucoidan regulated the invasion of OSCC cells and also their recruiting and re-educating effects on macrophages, suggesting it could be a complementary approach in the treatment of OSCC.

Fucoidan is a complex of polysaccharides showing antitumor and immunomodulation properties. Our previous studies found its regulation to myeloid immune cells, including macrophages. Aberrant infiltration and functions of macrophages are commonly found in oral squamous cell carcinoma (OSCC). In this study, we analyzed the effects of fucoidan on invasion of OSCC cells, and their regulation to macrophages, trying to evaluate its role as a potential therapy for OSCC. CAL27 and THP-1-derived macrophages were used as models for OSCC cells and tumor-infiltrated macrophages in the in vitro study, respectively. The effects of fucoidan on invasion of OSCC cells and their recruitment to macrophages were analyzed by transwell assay. KIF4A siRNA transfection was performed to investigate its role in fucoidan-modulated OSCC cells invasion. CCL3-neutralizing antibody was added into the conditioned medium of OSCC cells to evaluate its role in fucoidan-mediated macrophages recruitment and re-education. Fucoidan reduced the invasive potential of CAL27 cells with a decrease of MMP-2 and KIF4A transcription. KIF4A knockdown in CAL27 cells led to decreased invasion and MMP-2 expression. The conditioned medium of fucoidan-treated CAL27 cells promoted recruitment and inflammatory cytokines secretion on THP-1-derived macrophages. Further analysis found that fucoidan increased CCL3 production in CAL27 cells. Blocking CCL3 expression reversed the effects of fucoidan on macrophage recruitment and re-education. Our study found that fucoidan regulated the invasion of OSCC cells and also their recruiting and re-educating effects on macrophages, suggesting it could be a complementary approach in the treatment of OSCC.

Europe PMC is an archive of life sciences journal literature.

Fucoidan is a natural derived compound found in different species of brown algae and in some animals, that has gained attention for its anticancer properties. However, the exact mechanism of action is currently unknown. Therefore, this review will address fucoidans structure, the bioavailability, an …

Arabic gum (Acacia senegal, AG) is proven effective antioxidant and cytoprotective agent. The present study was designed to test this notion by investigating the possible role of AG against the radiographic contrast medium (Ioxitalamate, Telebrix-35®, TBX)-induced oxidative stress …

Background: Aloe vera is more effective than routine treatment alone to improve symptoms of phlebitis. However, few studies have been conducted in Indonesia to explore the effectiveness of aloe vera on phlebitis among hospitalized children with wide-ranged of age. The purpose of this study was to evaluate the effectiveness of aloe vera in reducing the degree of phlebitis among hospitalized children in Indonesia.Methods: This study was conducted using a quasi-experimental design, a pretest-posttest design with a control group. The sample of this study was 42 children (21 respondents in both groups) with phlebitis that hospitalized in one general public health in West Java, Indonesia from May to July 2019. The intervention group was provided a compress of extract aloe vera (5 ml) and the control group was provided a compress of 70% Alcohol. The instrument used in this study was Visual Infusion Phlebitis (VIP) score. The Mann Whitney test was used to evaluate the difference of degree of phlebitis between intervention and control group and Wilcoxon test used to evaluate the difference before and after the intervention.Results: The majority of respondents were in the infant stage (45.23%), having normal nutrition status (54.76%). On average, the length of stay in the hospital was seven days and receive intravenous therapy solution non-electrolyte and antibiotics. The average score for the post-test score was decreased in both classes. There is a statistically significant difference between the phlebitis score both in the Aloe Vera and 70% baseline-specific experimental classes, during 8, 16, 32, 40, 48 hours of treatment implementation (p

Fucoidans are complex polysaccharides derived from brown seaweeds which consist of considerable proportions of L-fucose and other monosaccharides, and sulphated ester residues. The search for novel and natural bioproduct drugs (due to toxicity issues associated with chemotherapeutics) has led to the …

We examined the effect of fucoidan, an immune modulator, on the phagocytic capacity of porcine peripheral blood polymorphonuclear cells (PMNs) exposed…

Introduction: Pruritus is one of the most common problems in patients with kidney failure. 60-80% of dialysis patients complain of itchy skin for which different therapies have been recommended. Medicinal plants compose a group of these therapies. This study aimed to evaluate the effect of aloe Vera gel on pruritus in ...

Mitochondria are considered to be the powerhouses of cells. They are the most commonly damaged organelles within dopaminergic neurons in patients with Parkinson's disease (PD). Despite the importance of protecting neuronal mitochondria in PD patients, the detailed mechanisms underlying mitochondrial …

Abstract. The sulfated polysaccharide fucoidan displays excellent anticancer properties with low toxicity in many kinds of cancers. However, its detailed pharma

Cisplatin-based therapy is a traditional, clinical treatment for cancers, including lung cancer. In this study, we found that sequential therapy, i.e.…

Cancer is most common in worldwide and badly threaten to human’s life. Unfortunately drugs, which are used for cancer therapy, are toxic and affect not only cancer cells but also normal cells and...

Chronic kidney disease (CKD) is a globally common and important disease and there are evidence for a bidirectional relationship between microbiota and…

Background: The prevalence of diabetes is rapidly rising worldwide at an alarming rate. Over the last 30 years, the status of diabetes has changed from being considered as a mild disorder of the elderly to one of the major causes of morbidity and mortality affecting the youth and middle aged people. Aloe vera has been used in folk medicine as a remedy for various diseases. It is extensively studied in treating diabetes mellitus. Aims and Objectives: This study evaluates the hypoglycemic effect of A. vera leaf dried powder and compares it with standard Metformin in alloxan-induced diabetic rats. Materials and Methods: Diabetes mellitus was experimentally induced in rats by intraperitoneal injection of alloxan monohydrate at a dose of 150 mg/kg. A pure extract of A. vera leaf was given orally once daily for 5 weeks in three graded doses of 100, 200, 400 mg/kg and compared with control (2 ml distilled water) and standard (metformin 50 mg/kg). Results: The elevated blood glucose levels in diabetic rats were reduced by the treatment with A. vera leaf extract at doses of 200 mg/kg and 400 mg/kg which was comparable to 50 mg/kg of metformin with no statistically significant difference (P < 0.0001). Conclusion: Our study concludes that A. vera leaf extract has a favorable effect in reversing alloxan induced hyperglycemia in rats.

Hepatocellular carcinoma (HCC) is a fast growing tumor associated with a high tendency for vascular invasion and distant metastasis. Recently, we repo…

Osteogenesis and angiogenesis, including cell–cell communication between blood vessel cells and bone cells, are essential for bone repair. Fucoidan is a chemical compound that has a variety of biolog...

There is a tendency nowadays to encourage the use of natural substances in dentistry. Aloe vera is one of these substances, which has recently gained …

Fucoidans are multifunctional marine macromolecules that are subjected to numerous and various downstream processes during their production. These processes were considered the most important abiotic factors affecting fucoidan chemical skeletons, quality, ...

David-desc