Comparison of acidity and metal ion affinity of D-Glucosamine and N-acetyl-D-glucosamine, a DFT study - ScienceDirect
The derivatives of glucose such as glucosamine (β-D-GlcN) and N-acetyl-D-β-glucosamine (GlcNAc) are significant in several biological systems. D-GlcN …
Suppression of norovirus by natural phytochemicals from Aloe vera and (...)
Human norovirus (HuNoV) is the major cause of viral foodborne diseases, but no effective medication is currently available. This study reported the an…
Effect of Glucosamine Conjugate-Functionalized Liposomes on Glioma Cell and Healthy Brain An Insight for Future Application in Brain Infusion SpringerLink
Conjugation of D-glucosamine with lipophilic moiety can ease its application in surface modification of liposomes. Interestingly, although D-glucosamine is safe, studies have shed light on “toxic effect” of its conjugates on cancer cells and highlighted its application in targeting glioma. However, understanding the safety of such conjugates for local delivery to the brain is unavailable. Herein, after successful synthesis of D-glucosamine conjugate (GC), the toxicity of functionalized liposome was evaluated both in vitro and in vivo. The study revealed a significant effect on cytotoxicity and apoptosis in vitro as assessed on grade IV-resistant glioma cell lines, SF268, U87MG, using MTT assay and PI staining. Additionally, this effect was not observed on normal human erythrocytes in the hemolysis assay. Furthermore, we demonstrated that GC liposomes were non-toxic to the normal brain tissues of healthy Sprague-Dawley rats. Successful functionalization yielded liposome with uniform particle size, stability, and cellular uptake. With
The Amazing Benefits That Aloe Vera Has • Chiropractic Scientists • 915-850-0900
• Aloe's prebiotic effects are fantastic due to the chemical structure of some of its components. Surprisingly the aloe vera gel is about 55% polysaccharides and contains a compound called acemannan.
Glucosamine Promotes Hepatitis B Virus Replication Through its Dual Effects in Suppressing Autophagic Degradation
Glucosamine (GlcN), a dietary supplement widely utilized to promote joint health and effective in the treatment of osteoarthritis, is an effective macroautophagy/autophagy activator in vitro and in vivo. Previous studies have shown that autophagy is required for hepatitis B virus (HBV) …
Efficacy of Glucosamine Sulphate in Skin Ageing Results from an ex viv (...)
Background: Glucosamine sulphate (GS) is essential in the biosynthesis of glycolipids, glycoproteins, glycosaminoglycans (GAGs), hyaluronate, and proteoglycans. Connective tissues primarily contain collagen and proteoglycans and play an important role in skin ageing. Objective: The objectives were to assess ex vivo the impact of GS on skin ageing p
The effect of Aloe vera ethanol extract on the growth inhibition of Candida albicans - PubMed
Aim Candida albicans can cause two major types of infections: superficial infections (such as oral or vaginal candidiasis) as well as life-threatening systemic infections, and Aloe vera extract is one of the potentially useful therapeutic options. The aim of this study was to determine …
Evaluation of the effect of N-acetyl-glucosamine administration on bio (...)
The present study aimed to evaluate the effect of N‑acetyl‑glucosamine (GlcNAc) on the joint health of healthy individuals without arthritic symptoms. A randomized double‑blind placebo‑controlled clinical trial was performed to investigate the effect of oral administration of a GlcNAc‑containing test supplement (low dose, 500 mg/day and high dose, 1,000 mg/day) on cartilage metabolism in healthy individuals with a mean age of 48.6±1.3 years (range, 23‑64 years) by analyzing the ratio of type II collagen degradation to type II collagen synthesis using type II collagen degradation (C2C) and synthesis (PIICP) markers. The results indicated that the changes in C2C/PIICP ratios from the baseline were suppressed in the treated with low and high doses of GlcNAc, compared with the placebo group at week 16 during intervention. To further elucidate the effect of GlcNAc, subjects with impaired cartilage metabolism were evaluated. Notably, the changes in the C2C/PIICP ratios were markedly suppressed in the groups treated with low and high doses of GlcNAc at week 16. Finally, to exclude the effect of heavy body weight on joint loading, subjects weighing
The effect of aloe vera on ischemia—Reperfusion injury of sciatic nerv (...)
Aloe vera is compound which has strong antioxidant and anti-inflammatory effects. We investigated the neuroprotective role of aloe vera treatment in r…
Evaluation of the effect of the administration of an N-acetyl-glucosam (...)
Background: To evaluate the chondroprotective action of an N-acetyl-glucosamine (GlcNAc)-containing supplement on the joint health of healthy individuals without symptoms of arthritis, we conducted a randomized double-blind placebo-controlled clinical trial. Methods: Subjects (n=100, 51.3 ± 1.0 years (mean ± SE)) without symptoms of arthritis were randomly assigned to receive a 1000 mg GlcNAc-containing diet (GlcNAc group) or a placebo diet (placebo group) once a day for 16 weeks, and the effect on the cartilage metabolism was evaluated by analyzing the ratio of type II collagen degradation to synthesis using type II collagen degradation (C2C) and synthesis (PIICP) markers. Results: The results indicated that the changes in the C2C/PIICP ratios from the baseline were slightly suppressed in the GlcNAc group compared with those in the placebo group at weeks 16 during the intervention and 4 weeks after the intervention. However, there was no significant difference between the two groups. To make the effect of GlcNAc even more clear, the subjects with joint loading and impaired cartilage metabolism were evaluated. Interestingly, the changes in the C2C/PIICP ratios from the baseline were significantly suppressed in the GlcNAc group compared with the placebo group at weeks 16 during the intervention and 4 weeks after the intervention. Moreover, test supplement-related adverse events were not essentially observed during and after the intervention. Conclusions: These observations suggest that the oral administration of GlcNAc at a dose of 1000 mg/day exerts a chondroprotective action on the healthy individuals by lowering the C2C/PIICP ratio, which indicates relative reduction of type II collagen degradation and increase of type II collagen synthesis, without apparent adverse effect. Key words: N-acetyl-glucosamine, biomarker, cartilage metabolism, joint health
Reconstitution of TGFBR2 in HCT116 colorectal cancer cells causes incr (...)
Transforming growth factor-β (TGF-β) signaling plays a key role in regulating normal cell growth and differentiation, and mutations affecting members …
In vitro and in vivo immunomodulatory effects of fucoidan compound agents - ScienceDirect
Fucoidan extracted from brown algae displays diverse biological activities. In the present study, fucoidan was mixed with Chinese herb extracts, and t…
In vitro induction of endoplasmic reticulum stress in human cervical adenocarcinoma HeLa cells by fucoidan - ScienceDirect
Fucoidan is a sulfated hetero-polysaccharide, found in cell-wall composition of brown algae. Recent studies have reported the role of fucoidan in the …
Glucosamine and Its Analogues as Modulators of Amyloid-ß Toxicity ACS Medicinal Chemistry Letters
In Alzheimer’s disease (AD), amyloid-β (Aβ) oligomers are considered key mediators of synaptic dysfunction and cognitive impairment. These unstable intermediate Aβ species can interfere with different cellular organelles, leading to neuronal cell death, through the formation of Ca2+-permeable membrane pores, impairment in the levels of acetylcholine neurotransmitters, increased insulin resistance, promotion of pro-inflammatory cascades, among others. Based on a series of evidences that indicate the key role of glycosaminoglycans (GAGs) in amyloid plaque formation, we evaluated the capacity of four monosaccharides, i.e., glucosamine (GlcN), N-acetyl glucosamine (GlcNAc), glucosamine-6-sulfate (GlcN6S), and glucosamine-6-phosphate (GlcN6P), to reduce the Aβ-mediated pathological hallmarks. The tested monosaccharides, in particular, GlcN6S and GlcN6P, were able to interact with Aβ aggregates, reducing neuronal cell death, Aβ-mediated damage to the cellular membrane, acetylcholinesterase activity, insulin resistance, and pro-inflammation levels.
Glucosamine as a Pain Reliving Agent in Planter Fasciitis Tiwari Research & Reviews A Journal of Health Professions
Plantar fasciitis is the most common cause of the plantar heel pain. Its main features are a pain, tenderness mostly on the medial aspect of the calcaneum near the sole of heel. There are many conservative methods for treatment of the lantar fasciitis which include rest, massage, night splints, orthoses, injections, cast NSAID, shock wave therapy. The glucosamine is one of the nutritional product which has a good anti- properties and is already in use for arthritis knee. Depending on anti-inflammatory analgesic property can also be used in planter fasciitis. In this study 17 patients were studied after giving fixed dose of 1200 mg of glucosamine. The evaluation was done on linkert pain score. The p-value in studied group was
Glucosamine Suppresses Osteoclast Differentiation through the Modulati (...)
Osteoclasts represent the only bone resorbing cells in an organism. In this study, we investigated the effect of glucosamine (GlcN), a nutrient used t …
Influence of emulsifier type on the in vitro digestion of fish oil-in- (...)
The influence of an anionic marine polysaccharide (fucoidan) on the gastrointestinal fate of emulsified fish oils stabilized by different types of nat…
Effects of Six Weeks Endurance Training and Aloe Vera Supplementation on COX-2 and VEGF Levels in Mice with Breast Cancer - PubMed - NCBI
The aim of this study was to determine effects of six weeks endurance training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer. For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Alo …
Influence of molecular weight on the properties of Sargassum muticum fucoidan - ScienceDirect
The crude fucoidan extracted during hydrothermal treatment of Sargassum muticum was fractionated in a sequence of membranes (100 to 5 kDa) operating a…
Effects of the Aqueous Extract of Aloe vera on the Morphological and P (...)
The Iranian Red Crescent Medical Journal (IRCMJ) is international, open access, peer-reviewed, monthly, and ISI- Journal, affiliated to Iranian Hospital- Dubai, publishes original scientific studies in English that have direct clinical significance on Basic Science, Clinical Medicine, and Disaster Management. The journal strives to release the original articles related to various disciplines of medicine, including Review Articles, Meta-Analysis, Systematic Reviews, Clinical and Experimental Trials, Case Reports, and Letters. All research articles published in the journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two anonymous referees. The journal strives to strengthen connections between research and practice, so enhancing professional development and improving practice within the field of medicine.
Insoluble dietary fiber derived from brown seaweed Laminaria japonica ameliorate obesity-related features via modulating gut microbiota dysbiosis in high-fat diet–fed mice
Gut microbiota (GM) is considered to play an important role in obesity. Additionally, the impact of dietary fiber (DF) consumption on GM has been well established. Brown seaweeds are known to be a rich source of DF. However, the effect of insoluble DFs (IDFs) alone from brown seaweed on obesity and GM remain
Interaction of stem cells with nano hydroxyapatite-fucoidan bionanocom (...)
The combination of bioceramics with biopolymers are playing major role in the construction of artificial bone. Hydroxyapatite (HA) has been extensivel…
JFB Free Full-Text Biomineralization of Fucoidan-Peptide Blends and (...)
Fucoidan (Fuc), a natural polysaccharide derived from brown seaweed algae, and gelatin (Gel) were conjugated to form a template for preparation of biomimetic scaffolds for potential applications in bone tissue regeneration. To the Fuc–Gel we then incorporated the peptide sequence MTNYDEAAMAIASLN (MTN) derived from the E-F hand domain, known for its calcium binding properties. To mimic the components of the extracellular matrix of bone tissue, the Fuc–Gel–MTN assemblies were incubated in simulated body fluid (SBF) to induce biomineralization, resulting in the formation of β-tricalcium phosphate, and hydroxyapatite (HAp). The formed Fuc–Gel–MTN–beta–TCP/HAP scaffolds were found to display an average Young’s Modulus value of 0.32 GPa (n = 5) with an average surface roughness of 91 nm. Rheological studies show that the biomineralized scaffold exhibited higher storage and loss modulus compared to the composites formed before biomineralization. Thermal phase changes were studied through DSC and TGA analysis. XRD and EDS analyses indicated a biphasic mixture of β-tricalcium phosphate and hydroxyapatite and the composition of the scaffold. The scaffold promoted cell proliferation, differentiation and displayed actin stress fibers indicating the formation of cell-scaffold matrices in the presence of MT3C3-E1 mouse preosteoblasts. Osteogenesis and mineralization were found to increase with Fuc–Gel–MTN–beta–TCP/HAP scaffolds. Thus, we have developed a novel scaffold for possible applications in bone tissue engineering.
