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De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism BMC Genomics Full Text
De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism BMC Genomics Full Text
Background Aloe vera is a perennial, succulent, drought-resistant plant that exhibits many pharmacological characteristics such as wound healing ability against skin burns, anti-ulcer, anti-inflammatory, anti-tumor, anti-viral, anti-hypercholesterolemic, anti-hyperglycemic, anti-asthmatic and much more. Despite great medicinal worth, little genomic information is available on Aloe vera. This study is an initiative to explore the full-scale functional genomics of Aloe vera by generating whole transcriptome sequence database, using Illumina HiSeq technology and its progressive annotation specifically with respect to the metabolic specificity of the plant. Results Transcriptome sequencing of root and leaf tissue of Aloe vera was performed using Illumina paired-end sequencing technology. De novo assembly of high quality paired-end reads, resulted into 1,61,733 and 2,21,792 transcripts with mean length of 709 and 714 nucleotides for root and leaf respectively. The non-redundant transcripts were clustered using CD-HIT-EST, yielding a total of 1,13,063 and 1,41,310 unigenes for root and leaf respectively. A total of 6114 and 6527 CDS for root and leaf tissue were enriched into 24 different biological pathway categories using KEGG pathway database. DGE profile prepared by calculating FPKM values was analyzed for differential expression of specific gene encoding enzymes involved in secondary metabolite biosynthesis. Sixteen putative genes related to saponin, lignin, anthraquinone, and carotenoid biosynthesis were selected for quantitative expression by real-time PCR. DGE as well as qRT PCR expression analysis represented up-regulation of secondary metabolic genes in root as compared to leaf. Furthermore maximum number of genes was found to be up-regulated after the induction of methyl jasmonate, which stipulates the association of secondary metabolite synthesis with the plant’s defense mechanism during stress. Various transcription factors including bHLH, NAC, MYB were identified by searching predicted CDS against PlantTFdb. Conclusions This is the first transcriptome database of Aloe vera and can be potentially utilized to characterize the genes involved in the biosynthesis of important secondary metabolites, metabolic regulation, signal transduction mechanism, understanding function of a particular gene in the biology and physiology of plant of this species as well as other species of Aloe genus.
·bmcgenomics.biomedcentral.com·
De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism BMC Genomics Full Text
UKnowledge - International Grassland Congress Proceedings Performance of emAcacia senegalem L. Untapped Wealth of Gum Arabic in Rangelands and Grasslands in Arid and Semi-Arid Region of India
UKnowledge - International Grassland Congress Proceedings Performance of emAcacia senegalem L. Untapped Wealth of Gum Arabic in Rangelands and Grasslands in Arid and Semi-Arid Region of India
Acacia senegal (Linn) Wild a member of Mimosaceae is a small tree of 3-6m in height with umbrella-shaped crown. It is a typical tree of Sahel in Africa from Senegal to red sea and essentially limited to the area between 110 and 160 North, with a wide range of rainfall 100 to 800mm. It spread widely in tropical Africa from Mozambique, Zambia to Somalia, Sudan, Ethiopia, Kenya, Tanzania and Nigeria, and in South Asia in India and Pakistan. In India it is a typical tree of arid regions with a low rainfall of 100-250mm. It is drought resistant and tolerates prolonged dry period of 10-11 months, with maximum temperature reaching 500C with strong winds, but susceptible to frost. It occurs mostly on sand stones and skeletal soils and widely distributed as interspersed species in most of the rangelands and grasslands in arid and semi-arid regions of India. World’s 90% gum Arabic is produced from Acacia senegal. The quality of gum is very superior as compared to gum from any other species of Acacia (Andreson, 1990). Nearly 90% of gum Arabic is produced by Republic of Sudan especially from (Kordafan). Production of gum Arabic is meagre in India, and contribution to the world production is negligible. The total annual output of gum Arabic is only 800 Mt compared to world production and consumption of 60,000-70,000 Mt. The domestic production is insufficient even for domestic consumption and more of it is imported from Sudan and Nigeria to meet India's requirements. Gum exudes from cracks in bark of trees, mostly in the dry season. In Sudan the annual yields from young trees ranges from 188 to 2856 g (av. 0.9 kg), and from older trees, 379 to 6754 g (av. 2.0 kg). In India, however, the productivity is low varying from 175 to 550g tree-1 year-1. The main gum producing regions of India where natural as well as planted stands of A. senegal occur are in desert and arid region of Rajasthan, Gujarat, Haryana, and Punjab. The gum yield from various Acacia trees in their natural habitat is very poor. In arid and semi-arid region of India, particularly in Rajasthan, Gujarat, Haryana, Punjab and Bundelkahand, there is a good scope for extending area for large- scale plantation for production of gum Arabic. The area covered under forest, barren and uncultivable, pasture, oren (temple lands) and community grazing land etc, can be used for
·uknowledge.uky.edu·
UKnowledge - International Grassland Congress Proceedings Performance of emAcacia senegalem L. Untapped Wealth of Gum Arabic in Rangelands and Grasslands in Arid and Semi-Arid Region of India
An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N- glycosylation status of serum glycoproteins in healthy subjects
An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N- glycosylation status of serum glycoproteins in healthy subjects
European Journal of Clinical Nutrition - An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N-glycosylation status of serum...
·nature.com·
An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N- glycosylation status of serum glycoproteins in healthy subjects
Why Sugars Matter in Fighting Disease - A Panel Discussion - YouTube
Why Sugars Matter in Fighting Disease - A Panel Discussion - YouTube
On Nov. 1, 2018, GlycoNet held a panel discussion, Why Sugars Matter in Fighting Disease, at the Li Ka Shing Centre for Health Research Innovation in Edmonton, Alberta. The panel discussion was MC'd by author and former Daily Planet host Jay Ingram. Panel members included: Dr. Mona Nemer, Canada’s Chief Science Advisor; Dr. Todd Lowary, Scientific Director, GlycoNet; Dr. Lori West, Professor, University of Alberta and Director, Canadian Donation and Transplant Research Program (CNTRP); Dr. David Vocadlo, Professor, Simon Fraser University; Dr. Karla Williams, Assistant Professor, University of British Columbia and Co-founder of GlyCa BioSciences and; Ms. Erum Razvi, PhD candidate, The Hospital for Sick Children.
·youtube.com·
Why Sugars Matter in Fighting Disease - A Panel Discussion - YouTube
The auxiliary effects of low-molecular-weight fucoidan on the quality of life for locally advanced rectal cancer patients receiving neoadjuvant concurrent chemoradiotherapy before surgery
The auxiliary effects of low-molecular-weight fucoidan on the quality of life for locally advanced rectal cancer patients receiving neoadjuvant concurrent chemoradiotherapy before surgery
Background Patients with cancer use low-molecular-weight fucoidan (LMF) as a supplement to therapy. However, most studies of LMF are in vitro or conducted using animals. Concurrent chemoradiotherapy (CCRT) is the gold standard for locally advanced rectal cancer (LARC). This s...
·researchsquare.com·
The auxiliary effects of low-molecular-weight fucoidan on the quality of life for locally advanced rectal cancer patients receiving neoadjuvant concurrent chemoradiotherapy before surgery