2016

NeuroMolecular Medicine - Neuroprotective, antineuroinflammatory, and proneurogenic effects of glucosamine, a naturally occurring amino sugar, have been reported in various animal models of brain...

In this study, a diabetic kidney disease model was established by placing the test rats on a high-sugar/high-fat diet combined with streptozotocin induction. Histopathological examination (H&E, Masson, and PASM stain) showed pathological changes in the diabetic rat kidneys, in addition to fibrotic symptoms and collagen deposition. Immunohistochemistry and western blot analyses indicated that the diabetic condition significantly increased the expressions of fibrotic markers including collagen, α-SMA, and fibronectin. The levels of cholesterol, triglyceride, and low-density lipoprotein were also increased in diabetic kidney disease (DKD) rat blood, while the level of high-density lipoprotein was decreased. The results of Oil red O staining experiments indicated that the kidneys of diabetic rats exhibited appreciable fat deposition, with high contents of triglyceride and cholesterol. To inhibit fibrosis and reduce fat deposition, low molecular weight fucoidan (LMWF) may be used. Based on PCR and western blot analyses, LMWF can regulate the expression levels of important lipid metabolism regulators, thereby impeding the development of kidney fibrosis. Through the vitro model, it also be indicated that LMWF could inhibit fibrosis process through regulating lipid metabolism which induced by palmitic acid.

Fucoidan is a polysaccharide found in brown alga with glorious potential for pharmacological activities, among which its anti-inflammatory properties have gained meaningful attention. Due to several advantages of formulations for topical application, this study aimed to develop and optimize a fucoidan-based cream formulation and to investigate its anti-inflammatory potential after topical application in vivo. Fucoidan from Fucus vesiculosus L. was used. The cream base consisting of olive oil and Kolliphor RH40 was optimized followed by in vitro agar diffusion and drug release studies. The fucoidan-based cream with 13% Kolliphor P 407, 1% Transcutol P, and 5% PEG400 showed good spreadability, washability, and colloidal stability, and it did not irritate the skin. The kinetics of fucoidan release from the optimized cream exhibited the best fit to the Korsmeyer–Peppas and Higuchi models with R2 > 0.99. Fucoidan release was controlled by drug diffusion and anomalous transport provided by the optimized cream base. The formulation was stable and provided high fucoidan release after storage for 1 year. Topical application of the fucoidan-based cream dose-dependently inhibited carrageenan-induced edema and ameliorated mechanical allodynia in rats. The efficacy of the fucoidan-based cream at a high dose was comparable with the efficacy of diclofenac gel. The fucoidan-based cream could be considered a promising anti-inflammatory formulation.

Background: Rheumatoid arthritis is a chronic inflammatory disease of autoimmune etiology. Current treatment includes non-steroidal anti-inflammatory drugs, selective cyclooxygenase-2 inhibitors, glucocorticoids, and disease-modifying antirheumatoid drugs, newer agents such as tumor necrosis factor-α inhibitors, and monoclonal antibodies have adverse effects such as peptic ulcer, renal toxicity, and hematological toxicity. Moreover, they are expensive when used on a long-term basis. Hence, it is necessary to find out the new cost effective as well as less toxic drug as alternative. Aim and Objective: The present study was taken up to evaluate antiarthritic activity of Aloe vera aqueous extract (AVE) in Wistar albino rats when used orally. Materials and Methods: A total 36 no. of healthy Wistar albino rats of weight 100–200 mg were used and divided into six different groups containing six rats in each. Into subplantar region of hind paw of all rats except normal control, 0.1 mL of complete Freund’s adjuvant (6 mg Mycobacterium butyricum suspended in heavy paraffin oil) was injected for induction of arthritis. The rats were treated with vehicle (gum acacia), standard drugs (indomethacin and dexamethasone) and test drug, that is, AVE 125 mg/kg, 250 mg/kg for 12 days. The rats were evaluated by measuring the % increase in paw edema volume and paw thickness (joint diameter), assessing the biophysical parameters (arthritic score), hematological parameters erythrocyte sedimentation rate, rheumatoid factor, and histopathological study of joints (arthritic index) for testing the antiarthritic potential. Results: AVE 250 mg/kg showed significant antiarthritic property as that of standard indomethacin and dexamethasone in terms of reduction in inflammation and arthritic scores. Conclusion: AVE at 250 mg/kg offered significant protection against arthritis and inflammation and can be a safer alternative for long term use.

Background. Aloe vera has long been considered an anticancer herb in different parts of the world. Objective. To explore the potential mechanism of aloe vera in the treatment of cancer using network pharmacology and molecule docking approaches. Methods. The active ingredients and corresponding protein targets of aloe vera were identified from the TCMSP database. Targets related to cancer were obtained from GeneCards and OMIM databases. The anticancer targets of aloe vera were obtained by intersecting the drug targets with the disease targets, and the process was presented in the form of a Venn plot. These targets were uploaded to the String database for protein-protein interaction (PPI) analysis, and the result was visualized by Cytoscape software. Go and KEGG enrichment were used to analyze the biological process of the target proteins. Molecular docking was used to verify the relationship between the active ingredients of aloe vera and predicted targets. Results. By screening and analyzing, 8 active ingredients and 174 anticancer targets of aloe vera were obtained. The active ingredient-anticancer target network constructed by Cytoscape software indicated that quercetin, arachidonic acid, aloe-emodin, and beta-carotene, which have more than 4 gene targets, may play crucial roles. In the PPI network, AKT1, TP53, and VEGFA have the top 3 highest values. The anticancer targets of aloe vera were mainly involved in pathways in cancer, prostate cancer, bladder cancer, pancreatic cancer, and non-small-cell lung cancer and the TNF signaling pathway. The results of molecular docking suggested that the binding ability between TP53 and quercetin was the strongest. Conclusion. This study revealed the active ingredients of aloe vera and the potential mechanism underlying its anticancer effect based on network pharmacology and provided ideas for further research.

Aloe vera extract and its bioactive compounds possess anti-proliferative properties against cancer cells. However, no detailed molecular mechanism of …

The ever-changing landscape of dieting and its correlation with health outcomes have continued to evolve with time. New diets appear and disappear just as quickly as they gain notoriety. This is...

Methods: Open-label, multicenter, retrospective, noncontrolled analysis of long-term visual acuity and safety in 111 LHON patients treated with idebenone (900 mg/day) in an expanded access program. Eligible patients had a confirmed mitochondrial DNA mutation and had experienced the onset of symptoms (most recent eye) within 1 year before enrollment. Data on visual acuity and adverse events were collected as per normal clinical practice. Efficacy was assessed as the proportion of patients with either a clinically relevant recovery (CRR) or a clinically relevant stabilization (CRS) of visual acuity. In the case of CRR, time to and magnitude of recovery over the course of time were also assessed. Results: At time of analysis, 87 patients had provided longitudinal efficacy data. Average treatment duration was 25.6 months. CRR was observed in 46.0% of patients. Analysis of treatment effect by duration showed that the proportion of patients with recovery and the magnitude of recovery increased with treatment duration. Average gain in best-corrected visual acuity for responders was 0.72 logarithm of the minimal angle of resolution (logMAR), equivalent to more than 7 lines on the Early Treatment Diabetic Retinopathy Study (ETDRS) chart. Furthermore, 50% of patients who had a visual acuity below 1.0 logMAR in at least one eye at initiation of treatment successfully maintained their vision below this threshold by last observation. Idebenone was well tolerated, with most adverse events classified as minor. Conclusions: These data demonstrate the benefit of idebenone treatment in recovering lost vision and maintaining good residual vision in a real-world setting. Together, these findings indicate that idebenone treatment should be initiated early and be maintained more than 24 months to maximize efficacy. Safety results were consistent with the known safety profile of idebenone....

Age-related macular degeneration (AMD), a leading cause of blindness in the United States, is said to be a ...

Scientific Reports - Weeklong improved colour contrasts sensitivity...

Covid Care At Synergy Health DPC, our goal is to keep you healthy at home Should you or any of your family seek to prevent becoming ill with Covid, experience the common symptoms of Covid or have lingering effects of Post Covid Syndrome, please be assured that we offer several different Covid protocol options that… Continue reading Covid Care

David Martin specialises in putting humanity back into humans - and business. David's secret of success globally is ‘The Wobble Effect’. Read more...

Checks  can be mailed to: Dr Paul's Fight c/o: Paul Thomas  11790 SW Barnes Rd. Suite 140 Portland Or 97225 Write DrPaulsFight in memo or on a piece of paper

We provide affordable concierge TeleHealth visits to all of our patients ages 12 and up. Our providers often can schedule an acute care visit with limited wait times. We also offer specialized services to address dementia, fibromyalgia and other long term conditions. Additionally, we are available as a health advocate should you or a loved one need hospital or specialist care

In this episode, we have a special surprise. We interviewed one of Dr. Seeds MD students in Cellular Medicine, Dr. Juan Bautista of North Fresno Primary Care, and Owner/Primary Care Physician of Bautista Medical Group, on his incredible experience in treating COVID-19 patients in Fresno on a budget. This truly mind bending conversation is about one of the themes of this podcast-- taking control of your health in your own hands. Dr. Bautista walks us through his priorities when treating patients with very limited budgets. It is a tough world out there, but we're thankful for Dr. Bautista who brings the quality of concierge care into an everyday home. We are in awe of this doctor, you have to hear why. Join us on social media! Facebook: https://www.facebook.com/drwilliamseeds/ Instagram: https://www.instagram.com/williamaseedsmd/ Pinterest: https://www.pinterest.com/williamseedsmd/ Twitter: https://twitter.com/williamseedsmd/

(NaturalHealth365) A union group is calling for COVID rapid tests to be pulled because the test swabs contain a toxic chemical.

Dr. Chetty explains everything is clear now.

Statistician Mathew Crawford talks to me about why he believes that the vaccines are completely ineffective based on the data. He also talks about gaming of the clinical trials to make the data look g

It is more than evident that a significant part of humanity is stampeding to take experimental vaccines for COVID-19. However, the “experimental” vaccines violate all 10 of the Nuremberg Codes, which carry the death penalty for those who seek to break these International Laws. Now, more than ever, we need new trials, not after the... View Article

In this intimate sit down interview with Wolfgang Wodarg, we discuss the broad issue of corruption in the WHO, how we should understand the "pandemic," or lack there of and how we must stop this diabo

A Mississippi doctor said he was fired for attempting to treat COVID-19 patients with ivermectin, which is approved ...

Click here to download the document as a PDF

welcome everyone we love you!

(NaturalHealth365) A U.S. Federal District Judge ruled that the EUA version of the Pfizer shot is not interchangeable with Comirnaty.