De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism BMC Genomics Full Text
Background Aloe vera is a perennial, succulent, drought-resistant plant that exhibits many pharmacological characteristics such as wound healing ability against skin burns, anti-ulcer, anti-inflammatory, anti-tumor, anti-viral, anti-hypercholesterolemic, anti-hyperglycemic, anti-asthmatic and much more. Despite great medicinal worth, little genomic information is available on Aloe vera. This study is an initiative to explore the full-scale functional genomics of Aloe vera by generating whole transcriptome sequence database, using Illumina HiSeq technology and its progressive annotation specifically with respect to the metabolic specificity of the plant. Results Transcriptome sequencing of root and leaf tissue of Aloe vera was performed using Illumina paired-end sequencing technology. De novo assembly of high quality paired-end reads, resulted into 1,61,733 and 2,21,792 transcripts with mean length of 709 and 714 nucleotides for root and leaf respectively. The non-redundant transcripts were clustered using CD-HIT-EST, yielding a total of 1,13,063 and 1,41,310 unigenes for root and leaf respectively. A total of 6114 and 6527 CDS for root and leaf tissue were enriched into 24 different biological pathway categories using KEGG pathway database. DGE profile prepared by calculating FPKM values was analyzed for differential expression of specific gene encoding enzymes involved in secondary metabolite biosynthesis. Sixteen putative genes related to saponin, lignin, anthraquinone, and carotenoid biosynthesis were selected for quantitative expression by real-time PCR. DGE as well as qRT PCR expression analysis represented up-regulation of secondary metabolic genes in root as compared to leaf. Furthermore maximum number of genes was found to be up-regulated after the induction of methyl jasmonate, which stipulates the association of secondary metabolite synthesis with the plant’s defense mechanism during stress. Various transcription factors including bHLH, NAC, MYB were identified by searching predicted CDS against PlantTFdb. Conclusions This is the first transcriptome database of Aloe vera and can be potentially utilized to characterize the genes involved in the biosynthesis of important secondary metabolites, metabolic regulation, signal transduction mechanism, understanding function of a particular gene in the biology and physiology of plant of this species as well as other species of Aloe genus.
EBSCOhost 120217311 The preventive effect of Rutin, Pine bark, Aloe Vera and Celery on oxidative stress factors and movement activity in focal cerebral ischemia model in rats-
UKnowledge - International Grassland Congress Proceedings Performance of emAcacia senegalem L. Untapped Wealth of Gum Arabic in Rangelands and Grasslands in Arid and Semi-Arid Region of India
Acacia senegal (Linn) Wild a member of Mimosaceae is a small tree of 3-6m in height with umbrella-shaped crown. It is a typical tree of Sahel in Africa from Senegal to red sea and essentially limited to the area between 110 and 160 North, with a wide range of rainfall 100 to 800mm. It spread widely in tropical Africa from Mozambique, Zambia to Somalia, Sudan, Ethiopia, Kenya, Tanzania and Nigeria, and in South Asia in India and Pakistan. In India it is a typical tree of arid regions with a low rainfall of 100-250mm. It is drought resistant and tolerates prolonged dry period of 10-11 months, with maximum temperature reaching 500C with strong winds, but susceptible to frost. It occurs mostly on sand stones and skeletal soils and widely distributed as interspersed species in most of the rangelands and grasslands in arid and semi-arid regions of India. World’s 90% gum Arabic is produced from Acacia senegal. The quality of gum is very superior as compared to gum from any other species of Acacia (Andreson, 1990). Nearly 90% of gum Arabic is produced by Republic of Sudan especially from (Kordafan). Production of gum Arabic is meagre in India, and contribution to the world production is negligible. The total annual output of gum Arabic is only 800 Mt compared to world production and consumption of 60,000-70,000 Mt. The domestic production is insufficient even for domestic consumption and more of it is imported from Sudan and Nigeria to meet India's requirements. Gum exudes from cracks in bark of trees, mostly in the dry season. In Sudan the annual yields from young trees ranges from 188 to 2856 g (av. 0.9 kg), and from older trees, 379 to 6754 g (av. 2.0 kg). In India, however, the productivity is low varying from 175 to 550g tree-1 year-1. The main gum producing regions of India where natural as well as planted stands of A. senegal occur are in desert and arid region of Rajasthan, Gujarat, Haryana, and Punjab. The gum yield from various Acacia trees in their natural habitat is very poor. In arid and semi-arid region of India, particularly in Rajasthan, Gujarat, Haryana, Punjab and Bundelkahand, there is a good scope for extending area for large- scale plantation for production of gum Arabic. The area covered under forest, barren and uncultivable, pasture, oren (temple lands) and community grazing land etc, can be used for
An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N- glycosylation status of serum glycoproteins in healthy subjects
European Journal of Clinical Nutrition - An open-label dosing study to evaluate the safety and effects of a dietary plant-derived polysaccharide supplement on the N-glycosylation status of serum...
Why Sugars Matter in Fighting Disease - A Panel Discussion - YouTube
On Nov. 1, 2018, GlycoNet held a panel discussion, Why Sugars Matter in Fighting Disease, at the Li Ka Shing Centre for Health Research Innovation in Edmonton, Alberta. The panel discussion was MC'd by author and former Daily Planet host Jay Ingram. Panel members included: Dr. Mona Nemer, Canada’s Chief Science Advisor; Dr. Todd Lowary, Scientific Director, GlycoNet; Dr. Lori West, Professor, University of Alberta and Director, Canadian Donation and Transplant Research Program (CNTRP); Dr. David Vocadlo, Professor, Simon Fraser University; Dr. Karla Williams, Assistant Professor, University of British Columbia and Co-founder of GlyCa BioSciences and; Ms. Erum Razvi, PhD candidate, The Hospital for Sick Children.
The Danger of Seed Oils & Finding a Healthier Option - w/ Jeff Nobbs | Empowering Neurologist EP159 - YouTube
We've had so many discussions that's far about the importance of dietary fat for health and longevity. But to be clear, the type of fat we consume is of incredible importance. These days our diets seem to be loaded with vegetable oils and seed oils, and as we will learn, these are generally quite threatening to our health. In fact vegetable oils are only recently introduced into human nutrition with global production increasing 1600% since the early 1900s.
There is a strong correlation between the increase consumption of vegetable oils and seed the oils with the incidence of severe chronic diseases including cardiovascular disease, diabetes and even various neurological conditions.
Today we are going to explore a new type of oil called cultured oil, prepared by fermentation and subsequent filtration. Leading this industry is a company called Zero Acres Farms, and as such, I have reach doubt to its co-founder and CEO, Jeff Nobbs, to explore exactly what this company is doing as well as the health benefits of their product.
====
TIMESTAMPS:
0:00 Intro
2:28 Realizing the Danger of Omega 6
10:10 Low Fat Diets
12:35 HNE
17:00 Inflammation & Lanolin Acid
20:38 Environmental Impact of Seed Oils
24:00 Saturated Fat
28:15 New Way to Produce Oils
33:36 What to Look for while buying
39:02 Conclusion
====
Jeff Nobbs:
Jeff Nobbs is the co-founder and CEO of Zero Acre Farms, a food company replacing destructive vegetable oils with healthier, more sustainable oils and fats made by fermentation. Prior to Zero Acre Farms, Jeff also co-founded the fast casual restaurant chain Kitava, food security non-profit HelpKitchen, and rewards shopping platform Extrabux. He has also served as COO for Perfect Keto and General Manager of Rakuten after it acquired Extrabux. Jeff blogs about health, nutrition, and sustainability at jeffnobbs.com
___________________________
Instagram: https://www.instagram.com/davidperlmutter/
Website: https://www.drperlmutter.com/
Subscribe to our channel:
https://www.youtube.com/channel/UCDRl_UAXxbHyOOjklnA0dxQ/?sub_confirmation=1
Why Everything You Learned About Viruses is WRONG GreenMedInfo
Groundbreaking research indicates that most of what we believed about the purportedly deadly properties of flu virus is based on nothing more than institutionalized superstition and myth.
TAIWAN No Lockdowns, No Closed Businesses, Non-WHO Member, and
Astonishingly, in spite of being only 80 miles from the coast of China with over 400,000 of its 24 million citizens working in China, as of mid-April, Taiwan only has 400 cases of COVID, 6 deaths