Glyconutrients

Malignant glioma (MG) is a poor prognostic brain tumor with inevitable recurrence after multimodality treatment. Searching for more effective treatment is urgently needed. Differentiation induction via epigenetic modification has been proposed as a potential anticancer strategy. Natural products are …

Obesity is global problem that contributes to disease, and is partly caused by fast-food, high-fat diets. Much attention has been focused on developing anti-obesity foods and chemical materials from natural sources. Seaweed has bioactive properties that influence immune activity and have anti-cancer and anti-obesity effects. Laminaria japonica is a widely consumed seaweed, and has been promoted as a health food in Korea. The bioactive properties of L. japonica include anti-cancer, anti-diabetic, and anti-inflammation effects. Most Laminaria japonica are distributed in a simple processing form such as drying, and their availability is very low. Therefore, various types of functional products can be developed if they can be applied to foods through functionalization using fermentation techniques. It is a structural problem that is the most problematic in seaweed processing. In this study, we used fermented Laminaria japonica. To increase physiological activity, fermentation treatment was performed to loosen the structure, thereby increasing the activity of the glycoprotein. First, we screened the anti-obesity potential of an L. japonica fermentation extract (LJF) using 3T3-L1 adipocyte cells. We determined cytotoxicity using an MTS assay and measured LJF for its ability to affect adipogenesis through glucose uptake, triglyceride levels, and Oil Red O staining. We confirmed that LJF inhibited adipocyte differentiation. CCAAT/enhancer-binding proteins α/β (C/EBP-α/β) and peroxisome proliferator-activated receptor-γ (PPAR-γ) are involved in the early and late stages of adipocyte differentiation. LJF significantly reduced the expression levels of C/EBP-α/β and PPAR-γ and decreased the concentration of adiponectin. Thus, our results suggest that LJF inhibits adipogenesis in 3T3-L1 cells, and may be valuable for its anti-obesity effects.

Background/Aims: The effect of treatment with gum acacia (GA), a prebiotic shown previously to ameliorate chronic kidney disease (CKD), in diabetic and non – diabetic rats with adenine – induced CKD has been investigated using several conventional and novel physiological, biochemical, and histopathological parameters. Methods: Diabetes mellitus was

Background/Aims: The effect of treatment with gum acacia (GA), a prebiotic shown previously to ameliorate chronic kidney disease (CKD), in diabetic and non – diabetic rats with adenine – induced CKD has been investigated using several conventional and novel physiological, biochemical, and histopathological parameters. Methods: Diabetes mellitus was

Objective: Based on anti-inflammatory effects of Aloe vera, the effect of aqueous extract of this plant on brain edema and changes in some pro-inflammatory cytokines was investigated after traumatic brain injury (TBI). Materials and Methods: In this study, adult male Wistar rats were divided into 5 groups: Sham, TBI, vehicle (Veh), and low dose (LA) and high dose (HA) Aloe vera. The vehicle and aqueous extract of Aloe vera were injected intraperitoneally 30 min after induction of diffuse TBI by Marmarou’s method. Brain edema (brain water content), and transforming growth factor beta (TGF-β), tumor necrosis factor alpha (TNFα), interleukin 6 (IL-6) and IL-1β levels in serum and brain were measured 24 hr after TBI induction. Results: Increased brain edema by TBI was reduced by both LA and HA (p

Treatment of many inflammatory diseases involves a chronic use of NSAIDs in large doses increasing acute kidney injury risk. This study was designed t…

Abstract Background Aloe vera is a plant that has been used as an alternative drug. This plant contains various compounds, like anthraquinone, saponin, flavonoid, alkaloid, and tannin that has an antibacterial effect against Staphylococcus aureus and Escherichia coli. Both of it were responsible for the infection incident. This study aims to determine the efficacy of Aloe vera sap as an antibacterial against Staphylococcus aureus and Escherichia coli. Methods An experimental study, in vitro using post-test only control group design, has been done at laboratory of Medical Faculty of Sriwijaya University, by examining the antibacterial activity of Aloe vera sap in five different concentration (5%, 10%, 20%, 40%, dan 80%) using well diffusion and solid dillusion method to determine the minimum bactericidal concentration (MBC). And then continued with the phytocemical screening to determine the compound inside the Aloe vera sap. Results Aloe vera sap were able to kill Staphylococcus aureus at 5% and Eshcerichia coli at 80%. Compatibility test showed that Aloe vera sap with concentration of 10%, 20%, 40%, and 80% are compatible with amoxicillin, therefore 80% is compatible with cefotaxime. This ability due to the compound that it contains, which is alkaloid, flavonoid, tannin, quinone, and saponin. Conclusion Aloe vera sap is effective as an antibacterial against Staphylococcus aureus and Escherichia coli.   Keyword: Aloe vera sap, antibacterial, efficacy, Staphylococcus aureus, Escherichia coli.  

Hydroactive wound dressings ensure a physiologically moist wound milieu which enhances healing and patient comfort. Polymers like polyurethane, salts of alginic acid, collagen, cellulose and other gelable polysaccharides are commonly used for preparation of hydrogels but have their own limitations. In the present study acacia gum, a natural polysaccharide having known antioxidant and wound healing properties has been blended with polyvinyl alcohol and prepared hydrogel matrix further explored for its wound healing potential. Structural characterization of blended films by FTIR and XRD method showed that polysaccharide gum associated with PVA molecules via acetal bridges and they were amorphous in nature. The gel exhibited free swell capacity of 64 g 0.100 cm−2, which is comparable to the commercially available films recommended for heavily exuding wounds. The GA/PVA blends showed 34% moisture retention (Rh) ability after 24 h and their fluid absorbing (26%) and fluid donation (16%) ability make them suitable for moist and fibrinous wounds. They also exhibited blood compatibility, oxygen permeability, bacterial impermeability, antioxidant activity, iron chelation ability and biodegradability. In vivo healing potential has been evaluated on Swiss albino mice where hydrogel coated wounds showed faster and scar-less wound regeneration. In vitro Povidone-Iodine loading and release studies showed that iodine release followed Fickian diffusion process. Prepared hydrogels are breathable and hydrophilic in nature and are able to maintain appropriate moisture level at wound surface, suitable for acceleration of wound healing process. Based upon the studied properties the designed hydrogels are recommended for dry, necrotic and low exuding wounds.

Background. Use of herbal compounds as an intracanal medicament in the field of endodontics has become noteworthy, one of which is the Aloe vera compound whose antibacterial effect has already been proven in the planktonic form of Enterococcus faecalis. The purpose of this study is to evaluate the antibacterial effect of Aloe vera on E. faecalis biofilms at the 4th and 6th week of development. Materials and Methods. 130 single root canal teeth without anomalies and caries were used. They were divided into two groups of 65 teeth for four and six weeks of biofilm production. Five samples of each group were examined for confirmation of biofilm formation under an electron microscope. Study groups were investigated with an antimicrobial agent as an intracanal medicament including 20 samples treated with Aloe vera, calcium hydroxide, and phosphate-buffered saline, and biofilm and survival of pathogens were investigated. Dentin chip suspensions were used for colony-forming unit (CFU) counting to estimate remaining E. faecalis counts. Results. The CFU mean in the 4th week subgroup in Aloe vera, phosphate-buffered saline, and calcium hydroxide was 0, 69166.66 ± 31688.58, and 25000 ± 30822.07, and in the 6th week, it was 136.36 ± 323.33, 95000 ± 12247.44, and 27501.66 ± 36570.34, respectively, which showed a significant difference between the used materials (). Conclusion. Aloe vera, in contrast to calcium hydroxide, eliminated 4th and 6th week biofilms and showed remarkable antibacterial properties against E. faecalis biofilm. These results support potency of Aloe vera to use as a natural antimicrobial material in the intracanal medicament.

Chronic kidney disease (CKD) is a significant risk factor for cardiovascular and peripheral vascular disease. Although mesenchymal stem cell (MSC)-bas…

Fucoidan is a bio-functional polysaccharide, mainly reported in brown seaweed. Diverse potential of fucoidan has created intense interest to review th…

Objective: To determine the activity of fucoidan from Undaria pinnatifida (UPF) and Fucus vesiculosus (FVF) when given in combination of chemotherapy drugs usin...

Fucoidan is a polysaccharide, which is derived from brown algae and some marine invertebrates, consisting mainly of L-fucose and sulfate ester groups [1]. Fucoidan is particularly found in the cell wall of marine brown algae. This polysaccharide is named as fucoidin when it is derived from the first marine brown algae. Kylin gave Fucoidin in 1913. However, this name has been changed to fucoidan according to IUPAC rules [2]. Fucoidan related different studies have performed in the literature [1,2]. It has different bioactivities such as anticoagulant, anti-thrombotic, antiinflammatory, antitumoral, immunomodulatory, anti-inflammatory, antioxidant, anti-hepatopathy, anti-uropathy, anti-inflammatory. These activities depend on the source of fucoidan samples taken from different species [1,2]. In addition, fucoidan is non-toxic or any adverse effects on the healthy tissues so that it can be used safety. There are many forms of fucoidan but the simplest molecular structure of fucoidan is obtained from Fucus vesiculosus consists mainly of 44.1% fucose, 26.3% sulphate and 31.1% ash and a small proportion of aminoglucose [3,4].

This research evaluated the potential of using the methanol extract of Aloe vera (L.) Burm.f (AVM) to prevent the formation of AGEs by means of the BSA/glucose assay, BSA-methylglyoxal assay, arginine-methylglyoxal assay, fructosamine, Nɛ-(carboxymethyl) lysine (CML), thiol groups, and …

GA has revealed potent antioxidative and anti-inflammatory properties in haemodialysis patients. Oral digestion of GA (30 g/day) decreased oxidative stress and inflammatory markers among haemodialysis patients. Trial registration. ClinicalTrials.gov Identifier: NCT03214692, registered 11 July 2017 ( …

ClinicalTrials.gov Identifier: NCT02467257. Registered 3rd June 2015.

The aim of current study is detect the activity role of Aloe vera and Opuntia ficus extract against certain pathogenic bacteria and evaluation of Aloe vera and O. ficus extract against certain pathogenic bacteria inhibition in vitro. 100 samples were obtained from patients with different diseases at age 6 Mon. to 65 years from both genders. This study was carried out in Lab. of Biology Dept., Science College/Tikrit University, at the period from 1st of June 2019 to end of January 2020. The antimicrobial activity was performed with the agar well diffusion method. The results show the ability of Aloe vera for inhibition of bacteria according on concentration and the concentrations of 100 was caused in inhibitory effect on E. coli, Acinetobacte, S. aureus, S. epidermidis and S. haemolyticus isolates at diameter 13, 12, 11, 9.8 and 15 mm respectively. While, Opuntia ficus extract at 100% concentration was caused in inhibitory effect bacteria isolates at diameter 10, 9.7, 8.2, 7 and 12 mm respectively. The results also show Aloe vera was found to be able for inhibition of bacteria concentration of 40% was caused in inhibitory effect on E. coli, Acinetobacte, S. aureus, S. epidermidis and S. haemolyticus isolates at diameter 14, 12, 11, 9 and 15 mm respectively. While, Opuntia ficus extract at 40% concentration was caused in inhibitory effect bacteria isolates at diameter 11, 10, 8, 7 and 13 mm respectively, while other concentrations 10%, 20, 30% show less inhibitory effect compare with 100% and 40% concentrations

Ho Park, and Beom-Su Kim. Korean J Clin Lab Sci. 2017;49:323-8. https://doi.org/10.15324/kjcls.2017.49.4.323

Gum Arabic (GA) is natural polysaccharide produced from the dried exudate of the acacia tree (Acacia senegal). Colonic microorganisms degrade GA to sh…