Glyconutrients

The aim of this study was to determine whether low-molecular-weight fucoidan (LMWF) can reduce TGF-β-mediated pathogenesis of tubulointerstitial fibro…

Aloe vera gel is a potential material as raw material industry, this is because a very complex composition. However Aloe vera gel is very easily oxidized or unstable. Viscosity gel and the benefit are decreased at room temperature after 24-36 hours. This research aims to obtain information about the resistance to oxidation via nitogren gas treatment and antioxidants, as well as the influence of phase changes in an attempt to retain the characteristics of the physicochemical Aloe vera gel over time. This Study can be described a conclusion that the best storage conditions are sound-proofed temperature conditions (4 ± 1)oc. Environmental conditioning by administering nitrogen gas storage and antioxidant Buthylated Hydroxytoluene (BHT) 750 ppm for 4 weeks defending the nature physicochemical Aloe vera gel. Freeze drying process of Aloe vera gel that has filled gum Arabic 3 % generates a more homogenous powder and smaller and more.

Low-molecular-weight Fucoidan (Oligo-Fucoidan) is a sulfated polysaccharide that has a variety of biological effects and has also been shown to have beneficial health effects. However, the molecular mechanisms underlying the therapeutic effects of Oligo-Fucoidan ...

The present investigation was carried out to evaluate the possible radioprotective potential of an Aloe vera extract against whole-body X-ray irradiation-induced testicular alterations in mice. Male ...

Background: Osteoarthritis (OA) is a degenerative disease with complex underling mechanisms 1–3. The interactions among several factors make the study of the disease very complex and often lead to different treatment, i.e. surgical or conservative, decisions for subjects clinically and radiographically similar. Recent explorations performed at the body level pointed out that macro-factors, like overweight or gait, can influence the development of the disease 4. The number of related factors is high, and they are very likely to interact with each other. However, the literature lacks randomized and balanced studies to verify such effects of multiple factors 5. Objectives: The aim of this work was to develop a multifactorial analysis to explore whether and how gait functionality and dynamics can be related to treatment decision. Methods: A multifactorial analysis of gait dynamics in OA subjects was developed. 81 OA subjects, graded 2-3 in KL, were selected based on 4 clinical factors: Gender (male – female), Age (60-67 – 68-75), BMI (25–29.9 – 30+) and Treatment (total knee replacement (TKR) – conservative treatment). Gait analysis was performed using 8 cameras BTS Smart-DX 700, 1.5 Mpixels 250 fps and 2 force plates BTS P-6000 500 Hz sampling (BTS S.p.A., Milan, Italy). Helen Hayes marker protocol with medial markers was used for the study. Each volunteer was asked to perform a minimum of 5 valid gait sequences. Functionality and dynamics parameters were measured. Functionality : Velocity of gait and the time needed to perform a gait cycle were computed. Dynamics: The reaction forces and torques at the ankles, knees and hips were computed through inverse dynamic analyses. Analysis of variance was performed for the four factors described among the functionality and dynamics parameters. Results: The multifactorial analysis showed that functionality values are more subjective to the studied factors than the dynamics ones. Functionality seems to be directly related to the clinical treatment. Patients who selected TKR needed more time to make a step, spent more time in double stand position and walked slower (p

The skin, being a cell-renewal system, is one of the first organs to be affected in total-body irradiation during radiotherapy. An attempt has been made in the present study to explore radiation-induced biochemical alterations caused by whole-body gamma irradiation and their modulation in Swiss albi …

Lung cancer is the most common cause of cancer‑associated mortality worldwide, and glucosamine has the potential to exhibit antitumor activity. To reveal its anti‑lung cancer mechanism, the present study investigated the effect of glucosamine on the transcriptional activity of forkhead box O (FOXO)1 …

The practice of bone implants is the standard procedure for the treatment of skeletal fissures, or to substitute and re-establish lost bone. A perfect…

Glucosamine (GlcN), also known as aminosaccharide, is an important functional monosaccharide and the first amino monosaccharide to be identified as a hydroxyl group of glucose substituted by amino ...

Objective To prospectively assess the association of habitual glucosamine use with risk of cardiovascular disease (CVD) events. Design Prospective cohort study. Setting UK Biobank. Participants 466 039 participants without CVD at baseline who completed a questionnaire on supplement use, which included glucosamine. These participants were enrolled from 2006 to 2010 and were followed up to 2016. Main outcome measures Incident CVD events, including CVD death, coronary heart disease, and stroke. Results During a median follow-up of seven years, there were 10 204 incident CVD events, 3060 CVD deaths, 5745 coronary heart disease events, and 3263 stroke events. After adjustment for age, sex, body mass index, race, lifestyle factors, dietary intakes, drug use, and other supplement use, glucosamine use was associated with a significantly lower risk of total CVD events (hazard ratio 0.85, 95% confidence interval 0.80 to 0.90), CVD death (0.78, 0.70 to 0.87), coronary heart disease (0.82, 0.76 to 0.88), and stroke (0.91, 0.83 to 1.00). Conclusion Habitual use of glucosamine supplement to relieve osteoarthritis pain might also be related to lower risks of CVD events.

Aim The aim was to study whether oral glucosamine hydrochloride (GlcN.HCl) or mucopolysaccharide protein (MucoP) has a structure-modifying effect on an anterior cruciate ligament transection (ACLT) ...

The present study is performed to investigate the effect of two different glucosamine containing drugs: Drug 1 and Drug 2 (D1 and D2) against CCl4 induced brain damage in male albino rats. Liverin (AM) was employed in the current study as an antioxidant reference drug. CCl4 administration caused a significant elevation in the levels of MDA and NO of brain tissue, in association with a significant decrease in the antioxidant defense system (GSH, SOD and GPX) that indicated the induction of oxidative stress in brain tissue. CCl4 administration induced brain injury as manifested by the obtained changes in neurotransmitter parameter (norepinephrine (NE), Dopamine (DA), Serotonin (5-HT), and Acetylcholinesterase AChE). The tested nutraceuticals and the antioxidant drug displayed a significant improvement against the undue effect of CCl4 via decreasing the brain tissue content of MDA, NO with the elevation of GSH content. Also, the significant increase in SOD and GPX enzymatic activity was obtained when compared to CCL4 group. In addition AM, D1, and D2 have an ameliorative effect on neurotransmitter parameter NE, DA, 5HT, and AChE. Results of this study suggest that both antioxidant drugs and tested nutraceuticals palliate the brain injuries through anti-oxidative effect, with the elimination of the deleterious effect of toxic metabolites of CCl4 on brain tissue.

Introduction Pharmaceuticals are a large and diverse group of compounds designed to prevent, cure, and treat disease, and improve health [1-2].  Significant fractions of the parent compound are excreted in un-metabolized form or as metabolites (active or inactive) into raw sewage and wastewater

Background: Glucosamine sulphate (GS) is essential in the biosynthesis of glycolipids, glycoproteins, glycosaminoglycans (GAGs), hyaluronate, and proteoglycans. Connective tissues primarily contain collagen and proteoglycans and play an important role in skin ageing. Objective: The objectives were to assess ex vivo the impact of GS on skin ageing p

N-acetylglucosamine, a simple sugar found in human breast milk and sold as an over-the-counter dietary supplement in the United States, promotes myelin repair in mouse models and correlates with myelination levels in multiple sclerosis patients, according to a new study.

UWill Discover 2018 Abstract Asma Ghafoor, Iraj Sadraei, John F. Trant Towards the synthesis of an acetal-free TF antigen from glucosamine Carbohydrates make up many of the key molecules in our body, especially those involved in signaling, and these systems are of growing interest in regard to better understanding and treating diseases. Carbohydrates are increasingly being found to play an important role in the immunogenic responses to different microbial infections and even to cancer. In terms of cancers, certain carbohydrates have been found to be expressed only on the surface of carcinomas: they are not found on healthy cells. The TF antigen, a simple disaccharide, is one such marker and is found on >85% of all carcinomas regardless of organ (ie breast, ovarian, cervical, prostate, lung, liver etc.); however, the immune system is not good at identifying carbohydrates. Consequently, we need to design new synthetic vaccines to draw attention to these targets so that the body can initiate an immune response to kill the cancer., Unfortunately, the highly biodegradable nature of carbohydrates, when exposed to various pH conditions and enzymes in the body, make it difficult to use carbohydrates in vivo. As a result, extensive and complex syntheses must be carried out to create disaccharides with better enzymatic resistance, so that they can exist long enough to initiate the immune response. The Trant Team hopes to create more stable carbohydrates by removing the unstable acetal group by replacing the exo-cyclic oxygen with a methylene group; this class of materials are known as C-glycosides. This presentation will explore our approach of using glucosamine as a starting product to produce this challenging target, and describe the potential application of this technology towards cancer therapy.

Tubulointerstitial fibrosis is recognized as a key determinant of progressive chronic kidney disease (CKD). Fucoidan, a sulphated polysaccharide extracted from brown seaweed, exerts beneficial effects in some nephropathy models. The present study evaluated the inhibitory effect of oligo-fucoidan (800 Da) on renal tubulointerstitial fibrosis. We established a mouse CKD model by right nephrectomy with transient ischemic injury to the left kidney. Six weeks after the surgery, we fed the CKD mice oligo-fucoidan at 10, 20, and 100 mg/kg/d for 6 weeks and found that the oligo-fucoidan doses less than 100 mg/kg/d improved renal function and reduced renal tubulointerstitial fibrosis in CKD mice. Oligo-fucoidan also inhibited pressure-induced fibrotic responses and the expression of CD44, β-catenin, and TGF-β in rat renal tubular cells (NRK-52E). CD44 knockdown downregulated the expression of β-catenin and TGF-β in pressure-treated cells. Additional ligands for CD44 reduced the anti-fibrotic effect of oligo-fucoidan in NRK-52E cells. These data suggest that oligo-fucoidan at the particular dose prevents renal tubulointerstitial fibrosis in a CKD model. The anti-fibrotic effect of oligo-fucoidan may result from interfering with the interaction between CD44 and its extracellular ligands.

Tubulointerstitial fibrosis is recognized as a key determinant of progressive chronic kidney disease (CKD). Fucoidan, a sulphated polysaccharide extracted from brown seaweed, exerts beneficial effects in some nephropathy models. The present study evaluated the inhibitory effect of oligo-fucoidan (800 Da) on renal tubulointerstitial fibrosis. We established a mouse CKD model by right nephrectomy with transient ischemic injury to the left kidney. Six weeks after the surgery, we fed the CKD mice oligo-fucoidan at 10, 20, and 100 mg/kg/d for 6 weeks and found that the oligo-fucoidan doses less than 100 mg/kg/d improved renal function and reduced renal tubulointerstitial fibrosis in CKD mice. Oligo-fucoidan also inhibited pressure-induced fibrotic responses and the expression of CD44, β-catenin, and TGF-β in rat renal tubular cells (NRK-52E). CD44 knockdown downregulated the expression of β-catenin and TGF-β in pressure-treated cells. Additional ligands for CD44 reduced the anti-fibrotic effect of oligo-fucoidan in NRK-52E cells. These data suggest that oligo-fucoidan at the particular dose prevents renal tubulointerstitial fibrosis in a CKD model. The anti-fibrotic effect of oligo-fucoidan may result from interfering with the interaction between CD44 and its extracellular ligands.

The derivatives of glucose such as glucosamine (β-D-GlcN) and N-acetyl-D-β-glucosamine (GlcNAc) are significant in several biological systems. D-GlcN …

The fucoidan with high anticancer activity was isolated from brown alga Fucus evanescens. The compound effectively prevented EGF-induced neoplastic cell transformation through inhibition of TOPK/ERK1/2/MSK 1 signaling axis. In vitro studies showed that the fucoidan attenuated mitogen-activated prote …

The pretreatment of aqueous extract of Aloe vera leaf in rats proved to act as a potential antioxidant which could be implicated toward protection of the integrity of liver of rat against pesticide i...

Laminaria japonica is widely cultivated in East Asia, including South Korea. Fucoidan, a main component of L. japonica, protects neurons from neurological disorders such as ischemia and traumatic brain injury. In the present study, we examined the effects of extract from fermented L. japonica on the reduction of proliferating cells and neuroblasts in mice that were physically (with electric food shock) or psychologically (with visual, auditory and olfactory sensation) stressed with the help of a communication box. Vehicle (distilled water) or fermented L. japonica extract (50 mg/kg) were orally administered to the mice once a day for 21 days. On the 19th day of the treatment, physical and psychological stress was induced by foot shock using a communication box and thereafter for three days. Plasma corticosterone levels were significantly increased after exposure to physical stress and decreased Ki67 positive proliferating cells and doublecortin immunoreactive neuroblasts. In addition, western blot analysis demonstrated that physical stress as well as psychological stress decreased the expression levels of brain-derived neurotrophic factor (BDNF) and the number of phosphorylated cAMP response element binding protein (pCREB) positive nuclei in the dentate gyrus. Fermentation of L. japonica extract significantly increased the contents of reduced sugar and phenolic compounds. Supplementation with fermented L. japonica extract significantly ameliorated the increases of plasma corticosterone revels and decline in the proliferating cells, neuroblasts, and expression of BDNF and pCREB in the physically stressed mice. These results indicate that fermented L. japonica extract has positive effects in ameliorating the physical stress induced reduction in neurogenesis by modulating BDNF and pCREB expression in the dentate gyrus.

Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity and its related diseases are unclear. This study demonstrated that LJPs prevented diet-induced obesity in a mice model, and improved obesity-related pa …

Polysaccharides from Laminaria japonica (LJPs) are reported to have various functional properties, but the effects and mechanisms of LJPs on obesity a…