Trehalose

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! One size does not fit all – Trehalose metabolism by Clostridioides difficile is variable across the five phylogenetic lineages | Microbiology Society
! One size does not fit all – Trehalose metabolism by Clostridioides difficile is variable across the five phylogenetic lineages | Microbiology Society
Clostridioides difficile , the leading cause of antibiotic-associated diarrhoea worldwide, is a genetically diverse species which can metabolise a number of nutrient sources upon colonising a dysbiotic gut environment. Trehalose, a disaccharide sugar consisting of two glucose molecules bonded by an α 1,1-glycosidic bond, has been hypothesised to be involved in the emergence of C. difficile hypervirulence due to its increased utilisation by the RT027 and RT078 strains. Here, growth in trehalose as the sole carbon source was shown to be non-uniform across representative C. difficile strains, even though the genes for its metabolism were induced. Growth in trehalose reduced the expression of genes associated with toxin production and sporulation in the C. difficile R20291 (RT027) and M120 (RT078) strains in vitro, suggesting an inhibitory effect on virulence factors. Interestingly, the R20291 TreR transcriptional regulatory protein appeared to possess an activator function as its DNA-binding ability was increased in the presence of its effector, trehalose-6-phosphate. Using RNA-sequencing analysis, we report the identification of a putative trehalose metabolism pathway which is induced during growth in trehalose: this has not been previously described within the C. difficile species. These data demonstrate the metabolic diversity exhibited by C. difficile which warrants further investigation to elucidate the molecular basis of trehalose metabolism within this important gut pathogen.
·microbiologyresearch.org·
! One size does not fit all – Trehalose metabolism by Clostridioides difficile is variable across the five phylogenetic lineages | Microbiology Society
1 Genetic Determinants of Trehalose Utilization Are Not Associated With Severe Clostridium difficile Infection Outcome | Open Forum Infectious Diseases | Oxford Academic
1 Genetic Determinants of Trehalose Utilization Are Not Associated With Severe Clostridium difficile Infection Outcome | Open Forum Infectious Diseases | Oxford Academic
Abstract. In a case–control study of patients with Clostridium difficile infection, we found no statistically significant association between the presence of tr
·academic.oup.com·
1 Genetic Determinants of Trehalose Utilization Are Not Associated With Severe Clostridium difficile Infection Outcome | Open Forum Infectious Diseases | Oxford Academic
1 Microbial and metabolic impacts of trehalose and trehalose analogues
1 Microbial and metabolic impacts of trehalose and trehalose analogues
Trehalose is a disaccharide and fasting-mimetic that has been both canonized and vilified for its putative cardiometabolic and microbial effects. Trehalose analogues are currently under development to extend the key metabolic therapeutic actions of trehalose ...
·ncbi.nlm.nih.gov·
1 Microbial and metabolic impacts of trehalose and trehalose analogues
1 Trehalose-Induced Remodelling of the Human Microbiota Affects Clostridioides difficile Infection Outcome in an In Vitro Colonic Model A Pilot Study
1 Trehalose-Induced Remodelling of the Human Microbiota Affects Clostridioides difficile Infection Outcome in an In Vitro Colonic Model A Pilot Study
Within the human intestinal tract, dietary, microbial- and host-derived compounds are used as signals by many pathogenic organisms, including Clostridioides difficile. Trehalose has been reported to enhance virulence of certain C. difficile ribotypes; ...
·ncbi.nlm.nih.gov·
1 Trehalose-Induced Remodelling of the Human Microbiota Affects Clostridioides difficile Infection Outcome in an In Vitro Colonic Model A Pilot Study
2 Clostridium difficile trehalose metabolism variants are common and not associated with adverse patient outcomes when variably present in the same lineage - eBioMedicine
2 Clostridium difficile trehalose metabolism variants are common and not associated with adverse patient outcomes when variably present in the same lineage - eBioMedicine
Trehalose metabolism variants are common in C. difficile. Increases in total dietary trehalose during the early-mid 2000s C. difficile epidemic were likely relatively minimal. Alternative explanations are required to explain why ribotype-027, ribotype-078 and ribotype-017 have been successful.
·thelancet.com·
2 Clostridium difficile trehalose metabolism variants are common and not associated with adverse patient outcomes when variably present in the same lineage - eBioMedicine
A head-to-head comparison review of biological and toxicological studies of isomaltulose, d-tagatose, and trehalose on glycemic control - PubMed
A head-to-head comparison review of biological and toxicological studies of isomaltulose, d-tagatose, and trehalose on glycemic control - PubMed
Diabetes mellitus is the most common metabolic disorder contributing to significant morbidity and mortality in humans. Different preventive and therapeutic agents, as well as various pharmacological strategies or non-pharmacological tools, improve the glycemic profile of diabetic patients. Isomaltul …
·pubmed.ncbi.nlm.nih.gov·
A head-to-head comparison review of biological and toxicological studies of isomaltulose, d-tagatose, and trehalose on glycemic control - PubMed
A neuroprotective dose of trehalose is harmless to metabolic organs: comprehensive histopathological analysis of liver, pancreas, and kidney | SpringerLink
A neuroprotective dose of trehalose is harmless to metabolic organs: comprehensive histopathological analysis of liver, pancreas, and kidney | SpringerLink
DARU Journal of Pharmaceutical Sciences - Trehalose is a non-reducing disaccharide synthesized by lower organisms. It has recently received special attention because of its neuroprotective...
·link.springer.com·
A neuroprotective dose of trehalose is harmless to metabolic organs: comprehensive histopathological analysis of liver, pancreas, and kidney | SpringerLink
A randomized crossover study comparing trehalosehyaluronate eyedrops and standard treatment patient satisfaction in the treatment of dry eye synd... - PubMed - NCBI
A randomized crossover study comparing trehalosehyaluronate eyedrops and standard treatment patient satisfaction in the treatment of dry eye synd... - PubMed - NCBI
Dry eye is a common disorder in routine ophthalmological practice, and a better understanding of the complex pathophysiology is leading to improved treatment. Thealoz Duo(®) is a novel artificial tear preparation containing two active ingredients: Trehalose, a naturally occurring disaccharide with a …
·ncbi.nlm.nih.gov·
A randomized crossover study comparing trehalosehyaluronate eyedrops and standard treatment patient satisfaction in the treatment of dry eye synd... - PubMed - NCBI
A randomized, controlled study of the efficacy and safety of a new eye (...)
A randomized, controlled study of the efficacy and safety of a new eye (...)
Hyaluronic acid-trehalose is effective and safe, with better patient satisfaction, than existing HA-only eyedrops particularly from the first month of treatment, and offers a therapeutic advancement in the treatment of moderate to severe DED.
·ncbi.nlm.nih.gov·
A randomized, controlled study of the efficacy and safety of a new eye (...)
Alleviation of cadmium-induced oxidative stress by trehalose via inhib (...)
Alleviation of cadmium-induced oxidative stress by trehalose via inhib (...)
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor that regulates a cluster of oxidative stress-inducible genes in cells. Here, we aimed to investigate whether trehalose (Tr...
·onlinelibrary.wiley.com·
Alleviation of cadmium-induced oxidative stress by trehalose via inhib (...)
Alleviation of cadmium-induced oxidative stress by trehalose via inhibiting the Nrf2-Keap1 signaling pathway in primary rat proximal tubular cells. - PubMed - NCBI
Alleviation of cadmium-induced oxidative stress by trehalose via inhibiting the Nrf2-Keap1 signaling pathway in primary rat proximal tubular cells. - PubMed - NCBI
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor that regulates a cluster of oxidative stress-inducible genes in cells. Here, we aimed to investigate whether trehalose (Tre) protects primary rat proximal tubular (rPT) cells against cadmium (Cd)-induced oxidative stress vi …
·ncbi.nlm.nih.gov·
Alleviation of cadmium-induced oxidative stress by trehalose via inhibiting the Nrf2-Keap1 signaling pathway in primary rat proximal tubular cells. - PubMed - NCBI
An impediment to random walk Trehalose microenvironment drives prefere (...)
An impediment to random walk Trehalose microenvironment drives prefere (...)
Developing effective theranostic nanoplex platforms for personalized disease treatment necessitates an understanding of and the ability to control live cell–nanoparticle interactions. However, aggregation of nanoparticles on the cell surface and their subsequent internalization is sparsely understood and adv
·pubs.rsc.org·
An impediment to random walk Trehalose microenvironment drives prefere (...)
An injectable particle-hydrogel hybrid system for glucose-regulatory insulin delivery. - PubMed - NCBI
An injectable particle-hydrogel hybrid system for glucose-regulatory insulin delivery. - PubMed - NCBI
Phenylboronic acid (PBA)-based polymeric hydrogels have been widely considered as one of the most promising insulin delivery system to replace the frequent insulin injections. However, these hydrogels, mostly based on a variety of PBA-containing acrylamide monomers, are still far from clinical reali …
·ncbi.nlm.nih.gov·
An injectable particle-hydrogel hybrid system for glucose-regulatory insulin delivery. - PubMed - NCBI
Antibiotics pave way for C. diff infections by killing bile acid-alter (...)
Antibiotics pave way for C. diff infections by killing bile acid-alter (...)
Bile acids, which are altered by bacteria normally living in the large intestine, inhibit the growth of Clostridium difficile, new research indicates. The work sheds light on the ways in which some commonly used antibiotics can promote C. diff infections by killing off the bile acid-altering microbes.
·sciencedaily.com·
Antibiotics pave way for C. diff infections by killing bile acid-alter (...)
Assessment of in vitro kinetics and biological impact of nebulized trehalose on human bronchial epithelium - PubMed
Assessment of in vitro kinetics and biological impact of nebulized trehalose on human bronchial epithelium - PubMed
Trehalose is added in drug formulations to act as fillers or improve aerosolization performance. Its characteristics as a carrier molecule have been explored; however, the fate of trehalose in human airway tissues has not been thoroughly investigated. Here, we investigated the fate of nebulized treh …
·pubmed.ncbi.nlm.nih.gov·
Assessment of in vitro kinetics and biological impact of nebulized trehalose on human bronchial epithelium - PubMed
Autophagy induction by trehalose Molecular mechanisms and therapeutic impacts. - PubMed - NCBI
Autophagy induction by trehalose Molecular mechanisms and therapeutic impacts. - PubMed - NCBI
The balance between synthesis and degradation is crucial to maintain cellular homeostasis and different mechanisms are known to keep this balance. In this review, we will provide a short overview on autophagy as an intracellular homeostatic degradative machinery. We will also describe the involvemen …
·ncbi.nlm.nih.gov·
Autophagy induction by trehalose Molecular mechanisms and therapeutic impacts. - PubMed - NCBI
Beneficial effects of trehalose on striatal dopaminergic deficits in rodent and primate models of synucleinopathy in Parkinson's disease. - PubMed - NCBI
Beneficial effects of trehalose on striatal dopaminergic deficits in rodent and primate models of synucleinopathy in Parkinson's disease. - PubMed - NCBI
Disease modification in Parkinson's disease (PD) is an unmet medical need. In the current study, we evaluated trehalose, a safe and well-tolerated disaccharide that has previously demonstrated efficacy in rodent models of neurodegenerative diseases, including PD. In a rat model of PD, based on deliv …
·ncbi.nlm.nih.gov·
Beneficial effects of trehalose on striatal dopaminergic deficits in rodent and primate models of synucleinopathy in Parkinson's disease. - PubMed - NCBI
Biomolecules Free Full-Text Trehalose for Ocular Surface Health
Biomolecules Free Full-Text Trehalose for Ocular Surface Health
Trehalose is a natural disaccharide synthesized in various life forms, but not found in vertebrates. An increasing body of evidence demonstrates exceptional bioprotective characteristics of trehalose. This review discusses the scientific findings on potential functions of trehalose in oxidative stress, protein clearance, and inflammation, with an emphasis on animal models and clinical trials in ophthalmology. The main objective is to help understand the beneficial effects of trehalose in clinical trials and practice, especially in patients suffering from ocular surface disease. The discussion is supplemented with an overview of patents for the use of trehalose in dry eye and with prospects for the 2020s.
·mdpi.com·
Biomolecules Free Full-Text Trehalose for Ocular Surface Health
Biosynthesis of Astrocytic Trehalose Regulates Neuronal Arborization i (...)
Biosynthesis of Astrocytic Trehalose Regulates Neuronal Arborization i (...)
Trehalose is a nonreducing disaccharide that has recently attracted much attention because of its ability to inhibit protein aggregation, induce autophagy, and protect against dissections and strokes. In vertebrates, the biosynthesis of trehalose was long considered absent due to the lack of annotated genes involved in this process. In contrast, trehalase (TreH), which is an enzyme required for the cleavage of trehalose, is known to be conserved and expressed. Here, we show that trehalose is present as an endogenous metabolite in the rodent hippocampus. We found that primary astrocytes were able to synthesize trehalose and release it into the extracellular space. Notably, the TreH enzyme was observed only in the soma of neurons, which are the exclusive users of this substrate. A statistical analysis of the metabolome during different stages of maturation indicated that this metabolite is implicated in neuronal maturation. A morphological analysis of primary neurons confirmed that trehalose is required for neuronal arborization.
·pubs.acs.org·
Biosynthesis of Astrocytic Trehalose Regulates Neuronal Arborization i (...)