2016

We evaluated Galectin-3 (Gal-3) as a potential early biomarker of acute kidney disease (AKI), and the effect of Gal-3 inhibition by modified citrus pe…

(NaturalHealth365) Research reveals how kidney function can remain healthy with good lifestyle choices. Find out how to protect your kidneys.

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The health effects of microplastics on the human body are taking center stage as water bottle use, billions of masks and PPE from the COVID response have flooded our environment with unprecedented contamination. Newly published studies show that microplastics may be contributing to blood clots and other health issues. Even more shocking- another study found […]

In a recent lecture, Dr. Peter McCullough presented alarming data related to COVID vaccines, the fraud of national health authorities, the ‘Therapeutic Nihilism’ being exercised in hospitals, and the urgent necessity of active resistance.

Bits of the transcriptome once believed to function as RNA molecules are in fact translated into small proteins.

MicroRNAs from plants accumulate in mammalian blood and tissues, where they can regulate gene expression.

Whether or not cannabis use may increase the risk for depression and/or anxiety is not clear. For one thing, it has not been possible to draw a defini…

Melatonin, a hormone secreted by pineal gland, exerts antimetastatic effects by reducing tumor cell proliferation, migration and invasion. MicroRNAs (miRNAs) are small, non-coding RNAs that play a crucial role in regulation of gene expression and biological processes of the cells. Herein, we search for a link between the tumor/metastatic-suppressive actions of melatonin and miRNA expression in triple-negative breast cancer cells. We demonstrated that melatonin exerts its anti-tumor actions by reducing proliferation, migration and c-Myc expression of triple negative breast cancer cells. By using Taqman-based assays, we analyzed the expression levels of a set of miRNAs following melatonin treatment of triple negative breast cancer cells and we identified 17 differentially expressed miRNAs, 6 down-regulated and 11 up-regulated. We focused on the anti-metastatic miR-148b and the oncogenic miR-210 both up-regulated by melatonin treatment and studied the effect of their modulation on melatonin-mediated impairment of tumor progression. Surprisingly, when miR-148b or miR-210 were depleted in triple-negative breast cancer cells, using a specific miR-148b sponge or anti-miR-210, melatonin effects on migration inhibition and c-myc downregulation were still visible suggesting that the increase of miR-148b and miR-210 expression observed following melatonin treatment was not required for the efficacy of melatonin action. Nevertheless, ours results suggest that melatonin exhibit a compound for metastatic trait inhibition, especially in MDA-MB-231 breast cancer cells even if a direct link between modulation of expression of certain proteins or miRNAs and melatonin effects has still to be established.